Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Ficus vasculosa Wall. ex Miq. Inhibits the LPS-Induced Inflammation in RAW264.7 Macrophages

  • Ji-Won, Park (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jin-Mi, Park (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Sangmi, Eum (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jung Hee, Kim (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jae Hoon, Oh (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jinseon, Choi (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Tran The, Bach (Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology) ;
  • Nguyen, Van Sinh (Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology) ;
  • Sangho, Choi (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kyung-Seop, Ahn (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jae-Won, Lee (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • Received : 2022.06.08
  • Accepted : 2022.08.30
  • Published : 2022.12.28
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Abstract

Ficus vasculosa Wall. ex Miq. (FV) has been used as a herbal medicine in Southeast Asia and its antioxidant activity has been shown in previous studies. However, it has not yet been elucidated whether FV exerts anti-inflammatory effects on activated-macrophages. Thus, we aimed to evaluate the ameliorative property of FV methanol extract (FM) on lipopolysaccharide (LPS)-induced inflammatory responses and the underlying molecular mechanisms in RAW264.7 macrophages. The experimental results indicated that FM decreased the production of inflammatory mediators (NO/PGE2) and the mRNA/protein expression of iNOS and COX-2 in LPS-stimulated RAW264.7 cells. FM also reduced the secretion of interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α and monocyte chemoattractant protein (MCP)-1 in LPS-stimulated RAW264.7 cells. Results also demonstrated that FM improved inflammatory response in LPS-stimulated A549 airway epithelial cells by inhibiting the production of cytokines, such as IL-1β, IL-6 and TNF-α. In addition, FM suppressed MAPK activation and NF-κB nuclear translocation induced by LPS. FM also upregulated the mRNA/protein expression levels of heme oxygenase-1 and the nuclear translocation of nuclear factor erythroid 2-related factor 2 in RAW264.7 cells. In an experimental animal model of LPS-induced acute lung injury, the increased levels of molecules in bronchoalveolar lavage (BAL) fluid were suppressed by FM administration. Collectively, it was founded that FM has anti-inflammatory properties on activated-macrophages by suppressing inflammatory molecules and regulating the activation of MAPK/NF-κB signaling.

Keywords

Acknowledgement

This research was supported by grants from KRIBB (Grant No. KGS123221), the Bio & Medical Technology Development Program of the National Research Foundation (NRF) and the Korean government (MSIT) (Grant. No. NRF-2020R1A2C2101228) of the Republic of Korea and the Vietnam Academy of Science and Technology, Project NVCC09.10/22-22.

References

  1. Huang X, Xiu H, Zhang S, Zhang G. 2018. The role of macrophages in the pathogenesis of ALI/ARDS. Mediators Inflamm. 2018: 1264913.
  2. Lee JW, Chun W, Lee HJ, Min JH, Kim SM, Seo JY, et al. 2021. The role of macrophages in the development of acute and chronic inflammatory lung diseases. Cells 10: 897.
  3. Eun SY, Seo J, Park SW, Lee JH, Chang KC, Kim HJ. 2014. LPS potentiates nucleotide-induced inflammatory gene expression in macrophages via the upregulation of P2Y2 receptor. Int. Immunopharmacol. 18: 270-276. https://doi.org/10.1016/j.intimp.2013.11.026
  4. Spadaro S, Park M, Turrini C, Tunstall T, Thwaites R, Mauri T, et al. 2019. Biomarkers for acute respiratory distress syndrome and prospects for personalised medicine. J. Inflamm. (Lond). 16: 1.
  5. Cao Z, Liu JL, Wu S, Wang Q. 2018. Mechanism of MCP-1 in acute lung injury and advanced therapy by drug-loaded dextrin nanoparticle. Int. J. Polym. Sci.. doi.org/10.1155/2018/9269154.
  6. Min JH, Kim SM, Park JW, Kwon NH, Goo SH, Ngatinem, et al. 2021. Lagerstroemia ovalifolia exerts anti-inflammatory effects in mice of LPS induced ALI via downregulating of MAPK and NF-κB activation. J. Microbiol Biotechnol. 31: 1501-1507. https://doi.org/10.4014/jmb.2107.07023
  7. Park JW, Ryu HW, Ahn HI, Min JH, Kim SM, Kim MG, et al. 2020. The Anti-inflammatory effect of Trichilia martiana C. DC. in the lipopolysaccharide-stimulated inflammatory response in macrophages and airway epithelial cells and in LPS-challenged mice. J. Microbiol. Biotechnol. 30: 1614-1625. https://doi.org/10.4014/jmb.2006.06042
  8. Kim SM, Min JH, Kim JH, Choi J, Park JM, Lee J, et al. 2022. Methyl p-hydroxycinnamate exerts anti-inflammatory effects in mouse models of lipopolysaccharide-induced ARDS. Mol. Med. Rep. 25: 37.
  9. Xu B, Wang H, Chen Z. 2021. Puerarin inhibits ferroptosis and inflammation of lung injury caused by sepsis in LPS induced lung epithelial cells. Front. Pediatr. 4: 706327.
  10. Lee JW, Park HA, Kwon OK, Park JW, Lee G, Lee HJ, et al. 2017. NPS 2143, a selective calcium-sensing receptor antagonist inhibits lipopolysaccharide-induced pulmonary inflammation. Mol. Immunol. 90: 150-157. https://doi.org/10.1016/j.molimm.2017.07.012
  11. Zhang C, Ning D, Pan J, Chen C, Gao C, Ding Z, et al. 2021. Anti-inflammatory effect fraction of Bletilla striata and its protective effect on LPS-induced acute lung injury. Mediators Inflamm. 2021: 6684120.
  12. Wu H, Yang Y, Guo S, Yang J, Jiang K, Zhao G, et al. 2017. Nuciferine ameliorates inflammatory responses by inhibiting the TLR4-mediated pathway in lipopolysaccharide-induced acute lung injury. Front. Pharmacol. 8: 939.
  13. Wang W, Yang QL, Shi YZ, Hou BY, Yang SQ, Huang H, et al. 2017. Protective effects of the ethanol extract of Viola tianshanica maxim against acute lung injury induced by lipopolysaccharides in mice. J. Microbiol. Biotechnol. 27: 1628-1638. https://doi.org/10.4014/jmb.1701.01067
  14. Lee JW, Kim MO, Song YN, Min JH, Kim SM, et al. 2022. Compound K ameliorates airway inflammation and mucus secretion through the regulation of PKC signaling in vitro and in vivo. J. Ginseng Res. 46: 496-504. https://doi.org/10.1016/j.jgr.2021.12.008
  15. Ren J, Li L, Wang Y, Zhai J, Chen G, Hu K. 2019. Gambogic acid induces heme oxygenase-1 through Nrf2 signaling pathway and inhibits NF-κB and MAPK activation to reduce inflammation in LPS-activated RAW264.7 cells. Biomed. Pharmacother. 109: 555-562. https://doi.org/10.1016/j.biopha.2018.10.112
  16. Lee W, Lee CH, Lee J, Jeong Y, Park JH, Nam IJ, et al. 2021. Botanical formulation, TADIOS, alleviates lipopolysaccharide (LPS)-Induced acute lung injury in mice via modulation of the Nrf2-HO-1 signaling pathway. J. Ethnopharmacol. 270: 113795.
  17. Park JW, Oh JH, Hwang D, Kim SM, Min JH, Seo JY, et al. 2021. 3,4,5-Trihydroxycinnamic acid exerts anti-inflammatory effects on TNF-α/IFN-γ-stimulated HaCaT cells. Mol. Med. Rep. 24: 509.
  18. Kim YS, Hwang JW, Jang JH, Son S, Seo IB, Jeong JH, et al. 2016. Trapa japonica pericarp extract reduces LPS-induced inflammation in macrophages and acute lung injury in mice. Molecules 21: 392.
  19. Yuan J, Li X, Fang N, Li P, Zhang Z, Lin M, et al. 2022. Perilla Leaf Extract (PLE) attenuates COPD airway inflammation via the TLR4/Syk/PKC/NF-κB pathway in vivo and in vitro. Front. Pharmacol. 12: 763624.
  20. Kim SM, Ryu HW, Kwon OK, Hwang D, Kim MG, Min JH, et al. 2021. Callicarpa japonica Thunb. ameliorates allergic airway inflammation by suppressing NF-κB activation and upregulating HO-1 expression. J. Ethnopharmacol. 267: 113523.
  21. Chi VV. 2012. Dictionary of medicinal plants in Vietnam. Medical Publishing House, Hanoi, 1: 849.
  22. National Institute of Medicinal Material. 2016. Checklist of medicinal plants in Vietnam. Science and Technology Publish House, pp. 332.
  23. Shi YX, Xu YK, Hu HB, Na Z, Wang WH. 2011. Preliminary assessment of antioxidant activity of young edible leaves of seven Ficus species in the ethnic diet in Xishuangbanna, Southwest China. Food Chem. 128: 889-894. https://doi.org/10.1016/j.foodchem.2011.03.113
  24. Bahri S, Ambarwati Y, Marlina L, Utami. 2020. Identification of phenyl propanoid compound isolated from root bark datuan (Ficus vasculosa Wall. Ex Miq) and antibacterial activity test on Escherichia coli. IOP Conf. Ser.: Earth. Environ. Sci. 537: 012046.
  25. Lee JW, Ryu HW, Kim DY, Kwon OK, Jang HJ, Kwon HJ, et al. 2021. Biflavonoid-rich fraction from Daphne pseudomezereum var. koreana Hamaya exerts anti-inflammatory effect in an experimental animal model of allergic asthma. J. Ethnopharmacol. 265: 113386.
  26. Nie Y, Wang Z, Chai G, Xiong Y, Li B, Zhang H, et al. 2019. Dehydrocostus lactone suppresses LPS-induced acute lung injury and macrophage activation through NF-κB signaling pathway mediated by p38 MAPK and Akt. Molecules 24: 1510.
  27. Kim SM, Ryu HW, Kwon OK, Min JH, Park JM, Kim DY, et al. 2022. Protective effect of Paulownia tomentosa fruits in an experimental animal model of acute lung injury. Microbiol. Biotechnol. Lett. 50: 310-318. https://doi.org/10.48022/mbl.2112.12007