대한약침학회지 (Journal of Pharmacopuncture)

  • 제25권4호
  • /
  • Pages.390-395
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  • 2022
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  • 2093-6966(pISSN)
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  • 2234-6856(eISSN)
대한약침학회 (KOREAN PHARMACOPUNCTURE INSTITUTE)
권호 표지
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Dual Cytotoxic Responses Induced by Treatment of A549 Human Lung Cancer Cells with Sweet Bee Venom in a Dose-Dependent Manner

  • Yu-Na, Hwang (Department of Biological Sciences, College of Natural Sciences, Kangwon National University) ;
  • In-Seo, Kwon (Department of Biological Sciences, College of Natural Sciences, Kangwon National University) ;
  • Han-Heom, Na (Department of Biological Sciences, College of Natural Sciences, Kangwon National University) ;
  • Jin-Sung, Park (Korean Pharmacopuncture Institute) ;
  • Keun-Cheol, Kim (Department of Biological Sciences, College of Natural Sciences, Kangwon National University)
  • 투고 : 2022.10.25
  • 심사 : 2022.11.30
  • 발행 : 2022.12.30
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초록

Objectives: Sweet bee venom (sBV) is purified from Apis mellifera, containing a high level of melittin-its main component. It has been used as a therapeutic agent for pain relief and anti-inflammation, as well as for treating neuronal abnormalities. Recently, there have been studies on the therapeutic application of sBV for anticancer treatment. In the present study, we investigated the pharmacological effect of sBV treatment in A549 human lung cancer cells. Methods: We used microscopic analysis to observe the morphological changes in A549 cells after sBV treatment. The MTT assay was used to examine the cytotoxic effect after dose-dependent sBV treatment. Molecular changes in sBV were evaluated by the expression of apoptosis marker proteins using western blot analysis. Results: Microscopic analysis suggested that the growth inhibitory effect occurred in a dose-dependent manner; however, cell lysis occurred at a concentration over 20 ㎍/mL of sBV. The MTT assay indicated that sBV treatment exhibited a growth inhibitory effect at a concentration over 5 ㎍/mL. On fluorescence activated cell sorting analysis, G0 dead cells were observed after G1 arrest at treatment concentrations up to 10 ㎍/mL. However, rapid cell rupture was observed at a concentration of 20 ㎍/mL. Western blot analysis demonstrated that sBV treatment modulated the expression of multiple cell death-related proteins, including cleaved-PARP, cleaved-caspase 9, p53, Bcl2, and Bax. Conclusion: sBV induced cell death in A549 human lung cancer cells at a pharmacological concentration, albeit causing hemolytic cell death at a high concentration.

키워드

과제정보

This work was supported by a fund (2016R1D1A3B02006754) and "Regional Innovation Strategy (2022RIS-005)" from the National Research Foundation funded by the Ministry of Education (MOE). In part, this work was also supported by a fund (2021R1I1A1A01047397).

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