Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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Modulation of Inula racemosa Hook Extract on Cardioprotection by Ischemic Preconditioning in Hyperlipidaemic Rats

  • Arun Kumar, Tiwari (Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences) ;
  • Pushpraj S, Gupta (Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences) ;
  • Mahesh, Prasad (Kamla Nehru Institute of Management and Technology) ;
  • Paraman, Malairajan (Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences)
  • Received : 2022.08.24
  • Accepted : 2022.10.13
  • Published : 2022.12.30
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Abstract

Objectives: Hyperlipidemia (HL) is a major cause of ischemic heart diseases. The size-limiting effect of ischemic preconditioning (IPC), a cardioprotective phenomenon, is reduced in HL, possibly because of the opening of the mitochondrial permeability transition pore (MPTP). The objective of this study is to see what effect pretreatment with Inula racemose Hook root extract (IrA) had on IPC-mediated cardioprotection on HL Wistar rat hearts. An isolated rat heart was mounted on the Langendorff heart array, and then ischemia reperfusion (I/R) and IPC cycles were performed. Atractyloside (Atr) is an MPTP opener. Methods: The animals were divided into ten groups, each consisting of six rats (n = 6), to investigate the modulation of I. racemosa Hook extract on cardioprotection by IPC in HL hearts: Sham control, I/R Control, IPC control, I/R + HL, I/R + IrA + HL, IPC + HL, IPC + NS + HL, IPC + IrA+ HL, IPC + Atr + oxidative stress, mitochondrial function, integrity, and hemodynamic parameters are evaluated for each group. Results: The present experimental data show that pretreatment with IrA reduced the LDH, CK-MB, size of myocardial infarction, content of cardiac collagen, and ventricular fibrillation in all groups of HL rat hearts. This pretreatment also reduced the oxidative stress and mitochondrial dysfunction. Inhibition of MPTP opening by Atr diminished the effect of IrA on IPC-mediated cardioprotection in HL rats. Conclusion: The study findings indicate that pretreatment with IrA e restores IPC-mediated cardioprotection in HL rats by inhibiting the MPTP opening.

Keywords

Acknowledgement

A special thanks goes out to KNIMT-FOP and Sam Higginbottom University in Prayagraj for making research facilities available to the writers. Thank you also go out to the department's student advisory group for their input on the paper.

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