Korean Journal of Acupuncture

  • 제39권4호
  • /
  • Pages.152-171
  • /
  • 2022
  • /
  • 2287-3368(pISSN)
  • /
  • 2287-3376(eISSN)
경락경혈학회 (Society for Meridian and Acupoint)
권호 표지
DOI QR코드

DOI QR Code

흰쥐에서 MIA로 유발된 골관절염에 선방활명음 가미방이 미치는 영향

Effects of Sunbanghwalmyung-Eum Gamibang on MIA-Induced Osteoarthritis in Rats

  • 신유빈 (대구한의대학교 한의과대학 침구의학교실) ;
  • 박한별 (대구한의대학교 한의과대학 침구의학교실) ;
  • 김재수 (대구한의대학교 한의과대학 침구의학교실) ;
  • 이현종 (대구한의대학교 한의과대학 침구의학교실) ;
  • 임성철 (대구한의대학교 한의과대학 침구의학교실) ;
  • 이윤규 (대구한의대학교 한의과대학 침구의학교실)
  • You Bin, Shin (Department of Acupuncture & Moxibustion, College of Korean Medicine, Daegu Haany University) ;
  • Han Byeol, Park (Department of Acupuncture & Moxibustion, College of Korean Medicine, Daegu Haany University) ;
  • Jae Su, Kim (Department of Acupuncture & Moxibustion, College of Korean Medicine, Daegu Haany University) ;
  • Hyun Jong, Lee (Department of Acupuncture & Moxibustion, College of Korean Medicine, Daegu Haany University) ;
  • Sung Chul, Lim (Department of Acupuncture & Moxibustion, College of Korean Medicine, Daegu Haany University) ;
  • Yun Kyu, Lee (Department of Acupuncture & Moxibustion, College of Korean Medicine, Daegu Haany University)
  • 투고 : 2022.11.03
  • 심사 : 2022.11.25
  • 발행 : 2022.12.27
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives : This study was designed to investigate the effects of Sunbanghwalmyung-eum gamibang on Monosodium iodoacetate-induced osteoarthritis rats. Methods : Forty Sprague-Dawley (SD) rats were divided into 5 groups of 8 rats each. Osteoarthritis (OA) was induced by injecting MIA (2 mg/50 µl) into the joint cavity of the left knee of SD rats belonging to the experimental group, and normal saline was injected into the joint cavity of the left knee instead of MIA in the normal group. To the normal group and the controlled group (OA group), 2 ml of distilled water was orally administered. To the positive control group (Indomethacin group), indomethacin 2 ml at a concentration of 2 mg/kg, to the low concentration group of SHG (Low group), 2 ml of SHG at a concentration of 2 mg/kg, and to the high concentration group of SHG (High group), 2 ml of SHG at a concentration of 4 mg/kg ml was orally administered. The drug was administered for a total of 4 weeks, and histological changes were analyzed by Hematoxylin-Eosin staining and Safranin-O staining. In addition, inflammatory cytokines such as TNF-α, IL-1β, and IL-6, and MMP-13, TIMP-1, and GAGs were immunohistochemically analyzed. Finally, hematological examination, blood biochemical examination, and liver and kidney biopsy were performed. Results : SHG groups (Low and High) inhibited the matrix destruction and damage of the knee joint cartilage in SD rat model, and significantly prevented the reduction in cartilage thickness. In SHG groups, the expressions of TNF-α, IL-1β, IL-6 and MMP-13 were significantly decreased, and the expressions of TIMP-1, GAGs were significantly increased compared with OA group. The safety indicators had no significant differences among five groups. Conclusions : These results show that SHG has cartilage protection capacity, anti-inflammatory effect.

키워드

참고문헌

  1. The Korean Orthopaedic Association. Orthopaedi cs. 7th ed. Seoul : Choishinuihaksa. 2013 : 1013-4.
  2. Yoh SB, Sul JU, Shin MS. Research trends on the treatment of knee osteoarthritis in Korean medicine. Korean J Acupunct. 2011 ; 28(1) : 139-55.
  3. Kim JR, Lee JH, Lee YK, Lee HJ, Kim JS. Anti-inflammatory and cartilage protection effects of Daeganghwal-tang in MIA-induced osteoarthritis at rats. Herbal Formula Science (HFS). 2021 ; 29(3) : 127-45. https://doi.org/10.14374/HFS.2021.29.3.127
  4. Hyeong K, Won JH, Woo CH. Anti-oxidant and anti-inflammatory effects of Chulbu-tang. Journal of Korean Medicine Rehabilitation. 2020 ; 30(3) : 71-87. https://doi.org/10.18325/jkmr.2020.30.3.71
  5. Woo CY, Kim YJ, Woo CH. Antioxidative, Anti-inflammatory effects of Jibaekjihwang-tang (zhibaidihuang-tang) on osteoarthritic rat model. Journal of Korean Medicine Rehabilitation. 2020 ; 30(2) : 1-18. https://doi.org/10.18325/jkmr.2020.30.2.1
  6. Lee KM, Jeong SH, Kim SJ, Seo IB. Effect of Samgieum treatment on the monosodium iodoacetate-induced osteoarthritis in rats. Journal of Korean Medicine Rehabilitation. 2008 ; 18(2) : 17-31.
  7. Kim DH, Jeong SH, Seo IB, Kim SJ. Suppression effects of Sopunghwalhyeol-tang on the monosodium iodoacetate-induced osteoarthritis in rats. Journal of Korean Medicine Rehabilitation. 2011 ; 21(1) : 57-77.
  8. Park WT. Effects of Daebangpung-tang on the monosodium iodoacetate-induced osteoarthritis in rats. Jecheon : Semyung University. 2008.
  9. Lee JT, Lee CE, Son JH, Lee IC, Lee JY, Park TS, et al. Cytotoxicity and physiological activity of SunbangHwalmyung-um. Kor. J, Herbology. 2005 ; 20(3) : 51-8.
  10. Chae BY. Study of the antiphlogistic, analgesic and antifebrile functions of Sunbangwhalmyung-Eum as used "One-Jeaw" Disease. Seoul : Kyung Hee University. 1980.
  11. Choe SI, Jo MJ, Kim SC, Byun SH. Inhibitory effect of Sunbanghwalmyungeum MeOH Extract on Pro-inflammatory mediator in lipopolysaccharide - activated Raw 264.7 Cells. Kor. J. Herbology. 2008 ; 23(3) : 11-8.
  12. Park MC, Hong SU. The effects of Sunbanghwalmyung-eum extract on anti-oxidant, anti-allergic and anti-inflammatory ability in mouse cell. The Journal of Korean Oriental Medical Ophthalmology&Otolaryngology&Dermatology. 2008 ; 21(2) : 46-53.
  13. Shin SM, Kim SM, Kim EI, Lee JE, Yoo DY. The experimental study on anti-inflammatory effects of Kagamsunbanghwalmyungeum (KSBH). The Journal of Oriental Obstetrics & Gynecology. 2007 ; 20(1) : 125-43.
  14. Lee BY, Ahn DK, Woo ER, Park HK. Studies on the antibacterial activity and active constituents of Sonbanghwalmyongum. JKM. 1998 ; 19(1) : 89-99.
  15. Hwang DY. Jeungmaeg Bangyaghabpyeon. Rev. ed. Seoul : Nam San Dang Publisher. 2021 : 326.
  16. Shin KM, Ko IG, Kim SE, Jin JJ, Hwang L, Kim SH, et al. Low-frequency electroacupncture improves locomotor function after sciatic crushed nerve injury in rats. J Exerc Rehabil. 2018 ; 14(6) : 927-33. https://doi.org/10.12965/jer.1836594.297
  17. Chen ZM. Furen daquan liangfang (Good Formulas for Women' with Annotations and Supplements). Shanghai : Shanghai Scientific and Technical Publishers. 1st ed. 1991 : 637.
  18. Lee C. Yixuerumen (Introduction to Medicine). 1st ed. Seoul : Uisungdang. 1994 : 756-8.
  19. Huh J. Treasured mirror of estern medicine. 3rd ed. Seoul : Daesung Publishing Company. 1999 : 391, 420.
  20. Lee BK. Diagnostic method on symptoms and treatment. 3rd ed. Seoul : Sungbo Publishers. 1999 : 318.
  21. Jo SH, Chun SC, Lim JH, Lee SG, Jee SY. Case of otitis media treated with Sonbanghwalmyongum. The Journal of Oriental Medical Opthalmology & Otolaryngology & Dermatology. 2003 ; 16(1) : 198-205.
  22. Park MC, Hong SU. The effects of Sunbanghwalmyung-eum extract on acute Sinusitis-induced mouse. JKM. 2009 ; 30(2) : 133-44.
  23. Choi YJ, Bae HJ, Hong SH. A case report of patient underwent debridement caused by cellulitis improved with Gamisunbangwhalmyung-Eum. The Journal of Korean Medical Opthalmology & Otolaryngology & Dermatology. 2013 ; 26(4) : 111-8. https://doi.org/10.6114/jkood.2013.26.4.111
  24. Korean Herbalogy Society. Herbalogy. 2nd ed. Seoul : Younglim. 2016 : 162, 205, 241, 244, 390, 449, 450, 466, 468, 478, 501, 584, 630, 634.
  25. CITES. Convention on international trade in endangered species of wild fauna and flora. Appendices I, II and III. 2021 : 19.
  26. Avwioro G. Histochemical uses of haematoxylin-a review. JPCS. 2011 ; 1 : 24-34.
  27. Wojdasiewicz P, Poniatowski LA, Szukiewicz D. The role of inflammatory and anti-inflammatory cytokines in the pathogenesis of osteoarthritis. Mediatiors Inflamm. 2014 ; 2014 : 561459. https://doi.org/10.1155/2014/561459
  28. Sohn DH, Sokolove J, Sharpe O, Erhart JC, Chandra PE, Lahey LJ, et al, Plasma proteins present in osteoarthritic synovial fluid can stimulate cytokine production via Toll-like receptor 4. Arthritis Res Ther. 2012 ; 14(1) : R7. https://doi.org/10.1186/ar3555
  29. Adamson A, Ghoreschi K, Rittler M, Chen Q, Sun HW, Vahedi G, et al. Tissue inhibitor of metalloproteinase 1 is preferentially expressed in Th1 and Th17 T-helper cell subsets and is a direct stat target gene. PLoS One. 2013 ; 8(3) : e59367. https://doi.org/10.1371/journal.pone.0059367
  30. Lambert E, Dasse E, Haye B, Petitfrere E. TIMPs as multifacial proteins. Crit Rev Oncol Hematol. 2004 ; 49(3) : 187-98. https://doi.org/10.1016/j.critrevonc.2003.09.008
  31. Hire JM, Evanson JL, Johnson PC, Zumbrun SD, Guyton MK, McPherson III JC, et al. Variance of matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase (TIMP) concentrations in activated, concentrated platelets from healthy male donors. J Orthop Surg Res. 2014 ; 9 : 29. https://doi.org/10.1186/1749-799X-9-29
  32. Helminen HJ, Hyttinen MM, Lammi MJ, Arokoski JP, Lapvetelainen T, Jurvelin J, et al. Regular joint loading in youth assists in the establishment and strengthening of the collagen network of articular cartilage and contributes to the prevention of osteoarthrosis later in life: a hypothesis. J Bone Miner Metab. 2000 ; 18(5) : 245-57. https://doi.org/10.1007/PL00010638