International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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The Juhwangjam (JH) 3rd day of fifth instar silkworm exhibits antioxidative properties and prevents high-fat diet-induced hypercholesterolemia

  • Ji Hae, Lee (Department of Agricultural Biology, National Institute of Agricultural Sciences, RDA) ;
  • Yeon-Ji, Kim (Korean Medicine-Application Center, Korea Institute of Oriental Medicine) ;
  • Kyungho, Kim (Korean Medicine-Application Center, Korea Institute of Oriental Medicine) ;
  • Hyun Bok, Kim (Department of Agricultural Biology, National Institute of Agricultural Sciences, RDA) ;
  • HaeYong, Kweon (Department of Agricultural Biology, National Institute of Agricultural Sciences, RDA)
  • Received : 2022.11.28
  • Accepted : 2022.12.16
  • Published : 2022.12.30
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Abstract

The silkworm is a food material that can simultaneously ingest phytochemicals from mulberry leaves, proteins with essential amino acids, and fatty acids. They are known to have hypoglycemic properties; however, further functional investigation is needed. In this study, four varieties of 3rd day of fifth instar silkworm with different cocoon colors, namely Baegokjam (BG), Goldensilk (GS), Yeonnokjam (YN), and Juhwangjam (JH), were compared in terms of antioxidant properties and cholesterol-lowering effect. JH, which had the highest polyphenol content (+38% vs. GS, p<0.05) showed high antioxidant efficacy. Treatment with JH also resulted in the lowest cholesterol biosynthesis enzyme activity (28% vs. control, p<0.05). In the animal study, high-fat diet (HFD)-fed mice that were orally administered JH extract for 12 weeks showed lower body weight gain (-10.4% vs. HFD, p<0.05) and serum total cholesterol levels (-12.7% vs. HFD, p<0.05). Comparing the varieties, JH had the highest effect. In future studies, analysis of the active ingredients according to their variety should be done.

Keywords

Acknowledgement

This research activity was supported by the 'Research Program for Agriculture Science and Technology Development' (Grant No. PJ01510702), National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.

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