Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of sheep slaughter age on myogenic characteristics in skeletal muscle satellite cells

  • Han, Yunfei (College of Food Science and Engineering, Inner Mongolia Agricultural University) ;
  • Guo, Wenrui (College of Veterinary Medicine, Inner Mongolia Agricultural University) ;
  • Su, Rina (Inner Mongolia Vocational college of Chemical Engineering) ;
  • Zhang, Yanni (College of Food Science and Engineering, Inner Mongolia Agricultural University) ;
  • Yang, Le (College of Food Science and Engineering, Inner Mongolia Agricultural University) ;
  • Borjigin, Gerelt (College of Food Science and Engineering, Inner Mongolia Agricultural University) ;
  • Duan, Yan (College of Food Science and Engineering, Inner Mongolia Agricultural University)
  • Received : 2021.04.26
  • Accepted : 2021.10.14
  • Published : 2022.04.01
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Abstract

Objective: The objective of this study was to investigate the effects of sheep slaughter age on myogenic characteristics in skeletal muscle satellite cells (SMSCs). Methods: Primary SMSCs were isolated from hind leg biceps femoris muscles of Wurank lambs (slaughtered at three months, Mth-3) and adults (slaughtered at fifteen months, Mth-15). SMSCs were selected by morphological observation and fluorescence staining. Myogenic regulatory factors (MRF) and myosin heavy chain (MyHC) expressions of SMSCs were analyzed on days 1, 3, 4, and 5. Results: The expressions of myogenic factor 5 (Myf5), myogenic differentiation (MyoD), Myf6, and myogenin (MyoG) in Mth-15 were significantly higher in Mth-15 than in Mth-3 on days 1, 3, and 4 (p<0.05). However, MyoG expression in Mth-15 was significantly lower than in Mth-3 on day 5 (p<0.05). The expressions of MyHC I, MyHC IIa, and MyHC IIx in Mth-15 were significantly higher than in Mth-3 on days 1 and 3 (p<0.05), and MyHC IIb were significantly lower than in Mth-3 on days 3 and 4 (p<0.05). In contrast, the expression of MyHC IIx in Mth-15 was significantly lower and MyHC IIb was significantly higher than in Mth-3 on days 5 (p<0.05). Conclusion: The slaughter age altered the expression of MRFs and MyHCs in SMSCs while differentiation, which caused the variation of myogenic characteristics, and thus may affect the meat quality of Wurank sheep.

Keywords

Acknowledgement

The authors are grateful for the support by Science and Technology Plan of the College of Food Science and Engineering, Inner Mongolia Agricultural University (SPKX201904), Inner Mongolia Natural Science Foundation of China (2021MS03012).

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