DOI QR코드

DOI QR Code

2021년 강원도 양식 무지개송어 및 은연어 비법정전염병 모니터링

Disease monitoring of cultured rainbow trout and coho salmon in Gangwon province in 2021

  • 우수지 (국립수산과학원 동해수산연구소 양식산업과) ;
  • 이승훈 (국립수산과학원 동해수산연구소 양식산업과) ;
  • 김소선 (국립수산과학원 동해수산연구소 양식산업과) ;
  • 변순규 (국립수산과학원 동해수산연구소 양식산업과) ;
  • 송준영 (국립수산과학원 병리연구과) ;
  • 황성돈 (한국해양대학교 해양과학융합학부)
  • Soo-ji, Woo (East Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Seung Hoon, Lee (East Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • So-Sun, Kim (East Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Soon-Gyu, Byun (East Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Joon-Young, Song (Pathology Research Division, National Institute of Fisheries Science) ;
  • Seong Don, Hwang (Division of Convergence on Marine Science, Korea Maritime and Ocean University)
  • 투고 : 2022.08.12
  • 심사 : 2022.10.24
  • 발행 : 2022.12.31

초록

Disease including parasite, bacteria and virus cause serious mortality to salmonid fish in the aquaculture. In this study, we investigated the current disease status of the rainbow trout (Oncorhynchus mykiss) and coho salmon (Oncorhynchus kisutch) in Yanayang, Pyeongchang, Jeongseon and Yeongwol of Gangwon province in 2021 and performed molecular characterization of those pathogen. For parasites, Ichthyophthirius multifiliis was observed at 2 farms. For bacteria, we identified Aeromonas sobria from kidney of rainbow trout using phylogenetic analysis of gyrB gene. A. salmonicida were isolated from necrosis site of gill cover and fin in coho salmon and necrotic lesion of fin in rainbow trout. Phylogenetic analysis using vap gene indicated that A. salmonicida isolated in this study were clustered with previously reported A. salmonicida subsp. salmonicida isolates. For virus, JRt-Nagano type of infectious haematopoietic necrosis virus was detected in rainbow trout, but infectious pancreatic necrosis virus and Oncorhynchus masou virus were not detected. These results provide useful information for the prevention of disease spread and transmission when cultivating new species such as Atlantic salmon in Korea.

키워드

과제정보

이 논문은 국립수산과학원 수산시험연구사업(R2022070)으로 수행되었습니다.

참고문헌

  1. Aso, Y., Wani, J., Klenner, D.A.S. and Yoshimizu, M.: Detection and identification of Oncorhynchus masou virus (OMV) disease by polymerase chain reaction (PCR). Bull. Fish. Sci. Hokkaido Univ., 52:111-116, 2001.
  2. Cho, M.Y., Won, K.M., Han, H.J., Kim, H.J., Jee, B.Y., Kim, S.R., Lee, S.J., Kim, J.W. and Park, M.A.: Currant Status of Detection of Aquatic Animal Pathogens in Cultured Juveniles for Stock Enhancement from 2009 to 2012. J. Fish Pathol., 26:99-110, 2013. https://doi.org/10.7847/JFP.2013.26.2.099
  3. Emmenegger, E. J., Meyers, T. R., Burton, T. O. and Kurath, G.: Genetic diversity and epidemiology of infectious hematopoietic necrosis virus in Alaska. Dis. Aquat. Org., 40: 163-176, 2000. https://doi.org/10.3354/dao040163
  4. Garver, K.A., Troyer, R.M. and Kurath, G.: Two distinct phylogenetic clades of infectious hematopoietic necrosis virus overlap within the Columbia River basin. Dis. Aquat. Org., 55:187-203, 2003. https://doi.org/10.3354/dao055187
  5. Garver, K.A., LaPatra, S.E., and Kurath, G.: Efficacy of an infectious hematopoietic necrosis (IHN) virus DNA vaccine in Chinook Oncorhynchus tshawytscha and sockeye O. nerka salmon. Dis. Aquat. Org., 64, 13-22, 2005. https://doi.org/10.3354/dao064013
  6. Gulla, S., Lund, V., Kristoffersen, A.B., Sorum, H. and Colquhoun, D.J.: vapA (A-layer) typing differentiates Aeromonas salmonicida subspecies and identifies a number of previously undescribed subtypes. J. Fish Dis., 39, 329-342, 2016. https://doi.org/10.1111/jfd.12367
  7. Han, H.J., Kim,D.Y., Kim, W.S., Kim, C.S., Jung S.J., Oh, M.J. and Kim, D.H. Atypical Aeromonas salmonicida infection in the black rockfish, Sebastes schlegeli Hilgendorf, in Korea. J. Fish Dis., 34:47-55, 2011. https://doi.org/10.1111/j.1365-2761.2010.01217.x
  8. Heppell, J., Berthiaume, L., Tarrab, E., Lecomte, J. and Arella, M.: Evidence of genomic variations between infectious pancreatic necrosis virus strains determined by restriction fragment profiles. J. Gen. Virol., 73, pp. 2863-2870, 1992. https://doi.org/10.1099/0022-1317-73-11-2863
  9. Hwang, S.D., Lee, D.W., Chun, W.J., Jeon, H.R., Kim, D.J., Hwang, J.Y., Seo, J.S., Kwon, M.G., Ji, H.S., Kim, J.N. and Jee, B.Y.: Disease monitoring of wild marine fish and crustacea caught from inshore and offshore Korea in 2018. Korean J. Environ. Biol., 37:474-482, 2019. https://doi.org/10.11626/KJEB.2019.37.4.474
  10. Kim, S.S., Kim, K.I., Yoo, H.K., Han, Y.S., Jegal, M.E., Byun, S.G., Lim, H.J., Park, J.S. and Kim Y.J.: Differential virulence of infectious hematopoietic necrosis virus (IHNV) isolated from salmonid fish in Gangwon Province, Korea. Fish. Shellfish Immunol., 119:490-498, 2021a. https://doi.org/10.1016/j.fsi.2021.10.038
  11. Kim, H.J.: Phylogenetic analysis of infectious hematopoietic necrosis virus (IHNV) isolated from cultured rainbow trout Oncorhynchus mykiss in Korea. J. Fish Pathol., 23:1-8, 2010.
  12. Kim, J.W., Lee, H.N., Jee, B.Y., Woo, S.H., Kim, Y.J. and Lee, M.K.: Monitoring of the mortalities in the aquaculture farms of South Korea. J. Fish Pathol., 25:271-277, 2012. https://doi.org/10.7847/JFP.2012.25.3.271
  13. Kim, J., Cho, M., Kim, K., Min, E., Lim, J. and Hong S.: Transcriptome profiling in head kidney of rainbow trout (Oncorhynchus mykiss) after infection with the low-virulent Nagano genotype of infectious hematopoietic necrosis virus. Arch. Virol.,166:1057-1070, 2021b. https://doi.org/10.1007/s00705-021-04980-9
  14. Kim, J., Cho M., Lim J., Choi, H. and Hong, S.: Pathogenic mechanism of a highly virulent infectious hematopoietic necrosis virus in head kidney of rainbow trout (Oncorhynchus mykiss) analyzed by RNA-Seq transcriptome profiling. Viruses 14:859, 2022.
  15. Kim, K.I., Cha, S.J., Lee, C., Baek,H.R., Hwang,S.D., Cho, M.Y., Jee, B.Y. and Park,M.A.: Genetic relatedness of infectious hematopoietic necrosis virus (IHNV) from cultured salmonids in Korea. Arch. Virol. 161:2305-2310, 2016. https://doi.org/10.1007/s00705-016-2913-9
  16. Kim, Y.S., Yoon, J.W., Han, H.J., Suebsing R. and Kim, J.H: Prevalence and Characterization of Typical Aeromonas salmonicida Chum Salmon Isolates in Korea. Fisheries and Aquatic Sciences, 14:347-354, 2011. https://doi.org/10.5657/FAS.2011.0347
  17. Kurath, G., Garver, K.A., Troyer, R.M., Emmenegger, E.J., Einer-Jensen, K. and Anderson, E.D.: Phylogeography of infectious hematopoietic necrosis virus in North America. J. Gen. Virol., 84: 803-814, 2003. https://doi.org/10.1099/vir.0.18771-0
  18. Lane, D.J. 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M, editors. Nucleic acid techniques in bacterial systematics. New York: Wiley. pp. 115-175, 1991.
  19. Lim, J. Go, E..and Hong S.; Phylogenetic classification and pathogenicity analysis of IHNV isolated from salmonids in Gangwon-do. J. Fish Pathol., 34:123-131, 2021.
  20. Lim, J. and Hong, S.; Characterization of Aeromonas salmonicida and A. sobria isolated from cultured salmonid fish in Korea and development of a vaccine against furunculosis. J Fish Dis., 43:609-620, 2020. https://doi.org/10.1111/jfd.13158
  21. Lim, J., Koo, B., Kim, K.I., Jeong, H.D. and Hong, S.; Genetic identification of Aeromonas species using a housekeeping gene, rpoD, in cultured salmonid fishes in Gangwon-Do. J. Fish Pathol., 30:79-88, 2017. https://doi.org/10.7847/JFP.2017.30.2.079
  22. Noble, A.C. and Summerfelt, S.T.: Diseases encountered in rainbow trout cultured in recirculating systems. Annual Review of Fish Diseases, 6:65-92, 1996. https://doi.org/10.1016/S0959-8030(96)90006-X
  23. Lund, V., Espelid, S. and Mikkelsen , H.: Vaccine efficacy in spotted wolffish Anarhichas minor: relationship to molecular variation in A-layer protein of atypical Aeromonas salmonicida. Dis. Aquat. Org., 56:31-42, 2003. https://doi.org/10.3354/dao056031
  24. Mochizuki, M., Kim, H.J., Kasai, H., Nishizawa, T. and Yoshimizu, M.: Virulence change of infectious hematopoietic necrosis against rainbow trout Oncorhynchus mykiss with viral molecular evolution. Fish Pathol., 44:159-165, 2009.
  25. Nilsen, H., Olsen, A.B., Vaagnes, O., Hellberg, H., Bottolfsen, K., Skjelstad, H. and Colquhoun D.J.: Systemic Flavobacterium psychrophilum infection in rainbow trout, Oncorhynchus mykiss (Walbaum), farmed in fresh and brackish water in Norway. J Fish Dis, 34:403-408, 2011.
  26. Olsen, A.B., Hjortaas, M., Tengs, T., Hellberg, H. and Johansen, R.: First Description of a New Disease in Rainbow Trout (Oncorhynchus mykiss (Walbaum)) Similar to Heart and Skeletal Muscle Inflammation (HSMI) and Detection of a Gene Sequence Related to Piscine Orthoreovirus (PRV). PLoS One, 10:e0131638, 2015.
  27. Tapia, D., Eissler, Y.,Torres, P., Jorquera, E., Espinoza J.C. and Kuznar J.: Detection and phylogenetic analysis of infectious pancreatic necrosis virus in Chile. Dis. Aquat. Organ., 116:173-184, 2015. https://doi.org/10.3354/dao02912
  28. Yanez, M.A., Catalan, V., Apraiz, D., Figueras, M.J. and Martinez-Murcia, A.J.: Phylogenetic analysis of members of the genus Aeromonas based on gyrB gene sequences. Int. J. Syst. Evol. Microbiol., 53: 875-883, 2003. https://doi.org/10.1099/ijs.0.02443-0
  29. Aquatic Organism Disease Control Act, 2021
  30. KOSIS(Korean statistical information service) https://kosis.kr/index/index.do