Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지
DOI QR코드

DOI QR Code

Production and characterization of monoclonal antibodies against white spot syndrome virus (WSSV)

White spot syndrome virus (WSSV)에 대한 단클론 항체 생산 및 특성 분석

  • Kyoung-Hui, Kong (Department of Aqualife Medicine, Chonnam National University) ;
  • Choon-Sup, Kim (Enbiogene) ;
  • Kim, Do-Hyung (Department of Aqualife life Medicine, Pukyong National University) ;
  • Wi-Sik, Kim (Department of Aqualife Medicine, Chonnam National University)
  • 공경희 (전남대학교 수산생명의학과) ;
  • 김춘섭 ((주)엔바이로젠) ;
  • 김도형 (부경대학교 수산생명의학과) ;
  • 김위식 (전남대학교 수산생명의학과)
  • Received : 2022.11.13
  • Accepted : 2022.12.05
  • Published : 2022.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

White spot syndrome virus (WSSV) is a prevalent and virulent pathogen affecting cultured whiteleg shrimp (Litopenaeus vannamei) in Korea. In this study, seven monoclonal antibodies (mAbs) (10A12, 16C3, 17G4, 21G5, 22C4, 23B6 and 24G6) were produced by using purified WSSV. The reactivity of these mAbs was analysed by Western blot (WB), indirect immunofluorescence (IIF), and lateral flow immunochromatographic assay (LFIA). WB analysis demonstrated that three mAbs (17G4, 22C4, and 23B6) reacted specifically to VP28 with an approximate molecular weight of 24 kDa, mAb 16C3 reacted with approximately 17 kDa. IIF analysis demonstrated specific fluorescence signals on gill tissues of WSSV-infected shrimp, with five mAbs (10A12, 16C3, 22C4, 23B6, and 24G6), pleopods from WSSV-infected shrimp were used for LFIA, where, two mAbs (21G5 and 22C4) exhibited positive reaction. In conclusion, it can be inferred that the mAbs usage and specificity depends on the nature of assay used for diagnosis.

Keywords

Acknowledgement

이 논문은 2019년도 해양수산부 재원으로 해양수산과학기술진흥원의 지원을 받아 수행된 연구임(20190392, 수산생물 검역용 신속 및 정밀진단 기술 개발).

References

  1. Huang, X., Aguilar, Z.P., Xu, H., Lai, W. and Xiong, Y.: Membrane-based lateral flow immunochromatographic strip with nanoparticles as reporters for detection: a review. Biosens. Bioelectron., 75: 166-180, 2016. https://doi.org/10.1016/j.bios.2015.08.032
  2. Huang, Y., Xu, T., Wang, W., Wen, Y., Li, K., Qian, L. and Liu, G.: Lateral flow biosensors based on the use of microand nanomaterials: a review on recent developments. Microchim. Acta, 187: 70, 2020.
  3. Jeong, H.N., Jang, M.S., Oh, M.J. and Kim, W.S.: Production of monoclonal antibodies against viral hemorrhagic septicemia virus (VHSV, genotype IVa) from olive flounder. J. Fish Pathol., 30: 149-154, 2017. https://doi.org/10.7847/JFP.2017.30.2.149
  4. Kim, J.W., Lee, H.N., Jee, B.Y., Woo, S.H., Kim, Y.J. and Lee, M.K.: Monitoring of the mortalities in the auqaculture farms of South Korea. J. Fish Pathol., 24(3): 271-277, 2012.
  5. Kim, W.S., Kim, S.W. and Oh, M.J.: Production of monoclonal antibodies against nervous necrosis virus (NNV, RGNNV genotype). J. Fish Aquat., 328-331, 2018.
  6. Kong, K.H., Jeong, H.N., Shyam, K.U., Oh, M.J., Kim, C.S., Kim, H.J. and Kim, W.S.: Development and validation of a lateral flow immunochromatographic assay for specific detection of viral hemorrhagic septicemia virus (VHSV, genotype IVa) in olive flounder (Paralichthys olivaceus). Aquaculture, 537: 736491, 2021.
  7. Kurien B.K., Dorri, Y., Dillon, S., Dsouza, A. and Scofield, R.H.: An overview of Werstern blotting for determining antibody specificities for immunohistochemistry. Sigle transduction immunohistochemistry, pp. 55-67, Kalyuzhny, A.E., Humana Press, New York, 2011.
  8. Liddell, J.E. and Cryer, A.: A practical guide to monoclonal antibodies, pp. 90-104, John Wiley and Sons, Inc., New York, 1991.
  9. Lo, C.P., Aoki, T., Bonami, J.R., Flegel, T., Leu, J.H., Lightner, D.V., Soderhall, K., Walker, P.J., Wang, H.C., Xun, X., Yang, F. and Vlak, J.M.: Ninth report of the international committee on taxonomy of viruses. Elsevier/Academic Press, London, pp. 229-234, 2011.
  10. National institute of fisheries science (NIFS).: Standard manual of whiteleg shrimp culture. 2018.
  11. National institute of fisheries science (NIFS).: Manual of diagnostic tests for aquatic life. 2021.
  12. Ngom, B., Guo, Y., Wang, X. and Bi, D.: Development and application of lateral flow test strip technology for detection of infectious agents and chemical contaminants: a review. Anal. Bioanal. Chem., 397: 1113-1135, 2010. https://doi.org/10.1007/s00216-010-3661-4
  13. Rajengran, K.V., Mukherjee, S.C., Vijayan, K.K., Jung, S.J., Kim, Y.J. and Oh, M.J.: A comparative study of white spot syndrom virus infection in shrimp from India and Korea. J. Invertebr. Pathol., 84: 173-176, 2004. https://doi.org/10.1016/j.jip.2003.10.003
  14. Shyam, K.U., Jeong, H.N., Oh, M.J., Kim, C.S. and Kim, W.S.: Development of a lateral flow immuno-chromatic strip assay for the detection of nervous necrosis virus (NNV, RGNNV genotype). Aquaculture, 520: 734944, 2020.
  15. Shyam, K.U., Kim, H.J., Oh, M.J., Kim, C.S., Kim, D.H. and Kim, W.S.: Antibody-based lateral flow chromatographic assays for detecting fish and shrimp pathogens: A technical review. Aquaculture, 558: 738345, 2022.
  16. Tsai, J.H., Wang, H.C., Leu, J.H., Hsiao, H.H., Wang, A.H.-J., Kou, G.H. and Lo, C.F.: Genomic and proteomic analysis of thirty-nine structural proteins of shrimp white spot syndrome virus. J. Virol., 78: 11360-11370, 2004. https://doi.org/10.1128/JVI.78.20.11360-11370.2004
  17. Vargas-Albores, F., Hinojosa-Baltazar, P., Portillo-Clark, G. and Magallόn-Barajas, F.: Influence of temperature and salinity on the yellowleg shrimp, Penaeus californiensis Holmes, prophenoloxidase system. Aquac. Res., 29: 549-553, 1998. https://doi.org/10.1111/j.1365-2109.1998.tb01166.x
  18. WOAH. Manual of Diagnostic tests for Aquatic Animals. White spot syndrom virus (WSSV); Office International des Epizooties: Paris, France, 2021.
  19. 김승호, 장규태: 단백질실험노트 (하), pp.17, pp129, 개정 3판, 월드사이언스, 서울, 2008.