융합정보논문지 (Journal of Convergence for Information Technology)

중소기업융합학회 (Convergence Society for SMB)
권호 표지
DOI QR코드

DOI QR Code

불레기말 추출물의 항산화 및 항염효과

Antioxidant and Anti-inflammatory activity of Colpomenia sinuosa extract

  • 조영재 ((주) 엘파운더) ;
  • 김숙희 (건국대학교 미래지식교육원 학점은행제 K뷰티산업융합학전공) ;
  • 최재영 (연성대학교 호텔외식조리과 호텔조리전공) ;
  • 이자복 ((주) 엘파운더)
  • Cho, Young Jae (L.FOUNDER INC) ;
  • Kim, Sook-hee (K-Beauty industry fusion, Konkuk Continuing Education Center, Konkuk University) ;
  • Choi, Jae Young (Dept. of Culinary Arts & Hotel Food Service-Major in Culinary Arts, Yeonsung University) ;
  • Lee, Ja-bok (L.FOUNDER INC)
  • 투고 : 2021.10.24
  • 심사 : 2022.01.22
  • 발행 : 2022.01.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 불레기말 추출물의 항산화능 및 항염활성을 확인하였다. 라디컬 소거능으로서 DPPH, ABTS, nitrite 소거능 실험, FRAP 실험, 항산화 물질 측정으로서 폴리페놀과 플라보노이드 농도 측정을 실시하였다. DPPH 실험에서는 2.821 mg ascorbic acid / g의 항산화능을 나타내었으며, ABTS 실험에서는 3.923 mg ascorbic acid / g의 항산화능을 나타내었으며, nitrite 소거능 실험에서는 17.89 mg ascorbic acid / g의 항산화능을 나타내었다. FRAP에서는 불레기말 추출물의 1 mg이 ascorbic acid 2.062±0.177 ㎍의 환원력을 보였다. 폴리페놀 농도는 62.85±3.18 mg/g으로 나타났다. 플라보노이드 농도는 10.01±0.24 mg/g으로 나타났다. 한편 세포실험에서는 세포독성과 항염활성을 알아보았다. 세포독성의 경우 20% 이하의 세포 독성을 보였으며, 100 ㎍/mL 농도에서 30.93±2.93%의 염증 억제능을 보여 불레기말 추출물의 기능성 화장품 원료로서의 가능성을 확인하였다.

In this study, the antioxidant and anti-inflammatory properties of Colpomenia sinuosa extracts were identified. Antioxidant experiments included DPPH, ABTS, nitrite scavenging experiments, and FRAP, polyphenol concentration measurements, flavonoid concentration measurements. DPPH assay results showed an antioxidant function of 2.821 mg ascorbic acid/g extract. ABTS assay results showed an antioxidant function of 3.923 mg ascorbic acid/g extract. nitrite assay results showed an antioxidant function of 17.89 mg ascorbic acid/g extract. In FRAP, 1 mg of the Colpomenia sinuosa extract showed a reduction of 2.062±0.177 ㎍ of ascorbic acid. For polyphenols, 62.85±3.18 mg/g was shown. Flavonoids showed 10.01±0.24 mg/g. In the meantime, cell experiments showed cytotoxicity and anti-inflammatory functions. In cytotoxicity experiments, Colpomenia sinuosa extracts showed cytotoxicity of less than 20% and an inflammatory inhibition of 30.93±2.93% at a concentration of 100 ㎍/mL. These results indicate that Colpomenia sinuosa extract is available as functional cosmetic material.

키워드

참고문헌

  1. K. M. Kim & J. D. Kim. (2004). A Study on Requirement and Degree of the Satisfaction about Cosmeceuticals of Women. Journal of the Society of Cosmetic Scientists of Korea, 30(4), 571-582.
  2. W. Brand-Williams, M. E. Cuvelier & C. L. W. T. Berset. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology, 28(1), 25-30. DOI : 10.1016/S0023-6438(95)80008-5
  3. A. A. Alfadda & R. M. Sallam. (2012). Reactive oxygen species in health and disease. Journal of Biomedicine and Biotechnology, 2012, 14. DOI : 10.1155/2012/936486
  4. J. S. Jeeva, J. Sunitha, R. Ananthalakshmi, S. Rajkumari, M. Ramesh & R. Krishnan. (2015). Enzymatic antioxidants and its role in oral diseases. Journal of pharmacy & bioallied sciences, 7(2), S331. DOI : 10.4103/0975-7406.163438
  5. M. Schieber & N. S. Chandel. (2014). ROS function in redox signaling and oxidative stress. Current biology, 24(10), R453-R462. DOI : 10.1016/j.cub.2014.03.034
  6. A. Kammeyer & R. M. Luiten. (2015). Oxidation events and skin aging. Ageing research reviews, 21, 16-29. DOI : 10.1016/j.arr.2015.01.001
  7. N. A. Vashi, M. B. D. C. Maymone & R. V. Kundu. (2016). Aging differences in ethnic skin. The Journal of clinical and aesthetic dermatology, 9(1), 31.
  8. H. Masaki. (2010). Role of antioxidants in the skin: anti-aging effects. Journal of dermatological science, 58(2), 85-90. DOI : 10.1016/j.jdermsci.2010.03.003
  9. J. Bonaventure, M. J. Domingues & L. Larue. (2013). Cellular and molecular mechanisms controlling the migration of melanocytes and melanoma cells. Pigment cell & melanoma research, 26(3), 316-325. DOI : 10.1039/c4fo00970c
  10. J. P. Ebanks, R. R. Wickett & R. E. Boissy. (2009). Mechanisms regulating skin pigmentation: the rise and fall of complexion coloration. International Journal of Molecular Sciences, 10(9), 4066-4087. DOI : 10.3390/ijms10094066
  11. E. Cevenini, D. Monti & C. Franceschi. (2013). Inflamm-ageing. Current Opinion in Clinical Nutrition & Metabolic Care, 16(1), 14-20. DOI : 10.1097/MCO.0b013e32835ada13
  12. E. S. Chambers & M. Vukmanovic-Stejic. (2020). Skin barrier immunity and ageing. Immunology, 160(2), 116-125. DOI : 10.1111/imm.13152
  13. F. A. Wagener, C. E. Carels & D. Lundvig. (2013). Targeting the redox balance in inflammatory skin conditions. International journal of molecular sciences, 14(5), 9126-9167. DOI : 10.3390/ijms14059126
  14. E. M. Balboa, E. Conde, A. Moure, E. Falque & H. Dominguez. (2013). In vitro antioxidant properties of crude extracts and compounds from brown algae. Food chemistry, 138(2-3), 1764-1785. DOI : 10.1016/j.foodchem.2012.11.026
  15. E. M. Balboa, E. Conde, A. Moure, E. Falque & H. Dominguez. (2013). In vitro antioxidant properties of crude extracts and compounds from brown algae. Food chemistry, 138(2-3), 1764-1785. DOI : 10.1016/j.foodchem.2012.11.026
  16. L. O'Sullivan, B. Murphy, P. McLoughlin, P. Duggan, P. G. Lawlor, H. Hughes & G. F. Gardiner. (2010). Prebiotics from marine macroalgae for human and animal health applications. Marine drugs, 8(7), 2038-2064. DOI : 10.3390/md8072038
  17. A. M. Gamal-Eldeen, E. F. Ahmed & M. A. Abo-Zeid. (2009). In vitro cancer chemopreventive properties of polysaccharide extract from the brown alga, Sargassum latifolium. Food and Chemical Toxicology, 47(6), 1378-1384. DOI : 10.1016/j.fct.2009.03.016
  18. S. Ananthi, H. R. B. Raghavendran, A. G. Sunil, V. Gayathri, G. Ramakrishnan & H. R. Vasanthi. (2010). In vitro antioxidant and in vivo anti-inflammatory potential of crude polysaccharide from Turbinaria ornata (Marine Brown Alga). Food and chemical toxicology, 48(1), 187-192. DOI : 10.1016/j.fct.2009.09.036
  19. C. Kim, Y. S. Kim, H. G. Choi & K. W. Nam. (2014). Variations of seaweed community structure and distribution of crustose coralline algae at Gallam, Samchuk, eastern coast of Korea. Korean Journal of Environment and Ecology, 28(1), 10-23. DOI : 10.13047/KJEE.2014.28.1.10
  20. M. S. Blois. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199-1200. https://doi.org/10.1038/1811199a0
  21. R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang & C. Rice-Evans. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine, 26(9-10), 1231-1237. DOI : 10.1016/S0891-5849(98)00315-3
  22. G. C. Jagetia & M. S. Baliga. (2004). The evaluation of nitric oxide scavenging activity of certain Indian medicinal plants in vitro: a preliminary study. Journal of Medicinal Food, 7(3), 343-348. DOI : 10.4014/kjmb.1409.09006
  23. A. Pekal & K. Pyrzynska. (2014). Evaluation of aluminium complexation reaction for flavonoid content assay. Food Analytical Methods, 7(9), 1776-1782. DOI : 10.1007/s12161-014-9814-x
  24. B. Alexander, D. J. Browse, S. J. Reading & I. S. Benjamin. (1999). A simple and accurate mathematical method for calculation of the EC50. Journal of pharmacological and toxicological methods, 41(2-3), 55-58. DOI : 10.1016/S1056-8719(98)00038-0
  25. S. H. Shin & S. M. Kang. (2021). The Antioxidation Effect of Brown Algae Extract. Journal of the Korean Society of Cosmetology, 27(4), 851-858. DOI : 10.52660/JKSC.2021.27.4.851
  26. C. S. Kwak, S. Kim & M. S. Lee. (2005). The correlation of antioxidative effects of 5 Korean common edible seaweeds and total polyphenol content. Journal of the Korean Society of Food Science and Nutrition, 34(8), 1143-1150. DOI : 10.3746/jkfn.2005.34.8.1143
  27. A. Cumashi, N. A. Ushakova, M. E. Preobrazhenskaya, A. D'Incecco, A. Piccoli, L. Totani & N. E. Nifantiev. (2007). A comparative study of the anti-inflammatory, anticoagulant, antiangiogenic, and antiadhesive activities of nine different fucoidans from brown seaweeds. Glycobiology, 17(5), 541-552. DOI : 10.1093/glycob/cwm014
  28. K. S. Kim & S. H. Kim. (2021). Antioxidant and Anti-inflammatory activity of Silvetia siliquosa extract. Journal of Convergence for Information Technology, 11(8), 232-239. DOI : 10.22156/CS4SMB.2021.11.08.232
  29. E. Apostolova, P. Lukova, A. Baldzhieva, P. Katsarov, M. Nikolova, I., Iliev & V. Kokova. (2020). Immunomodulatory and anti-inflammatory effects of fucoidan: a review. Polymers, 12(10), 2338. DOI : 10.3390/polym12102338