Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Effect of insect-resistant genetically engineered (Bt-T) rice and conventional cultivars on the brown planthopper (Nilaparvata lugens Stål)

  • Sung-Dug, Oh (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Eun Ji, Bae (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kijong, Lee (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Soo-Yun, Park (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Myung-Ho, Lim (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Doh-Won, Yun (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Seong-Kon, Lee (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Gang-Seob, Lee (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Soon Ki, Park (School of Applied Biosciences, Kyungpook National University) ;
  • Jae Kwang, Kim (Division of Life Sciences, Incheon National University) ;
  • Sang Jae, Suh (School of Applied Biosciences, Kyungpook National University)
  • Received : 2022.05.24
  • Accepted : 2022.07.18
  • Published : 2022.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Insect-resistant transgenic rice (Bt-T) expresses a toxic protein (mcry1Ac1) derived from the soil bacterium Bacillus thuringiensis found in the rice cultivar Dongjin with an insecticidal property against rice leaf roller (Cnaphalocrocis medinalis). In this study, to investigate the impact of Bt-T on non-target organisms, the feed and oviposition preferences and biological parameters of brown planthopper (Nilaparvata lugens Stål) were comparatively analyzed in four rice cultivars: Dongjin (parent variety), Ilmi (reference cultivar), Chinnong (brown planthopper resistant cultivar) and Bt-T. In the Bt-T and Dongjin cultivars, the feed preferences were 32.4 ± 8.3 and 34.1 ± 6.8%, and the oviposition preferences were 32.5 ± 5.1 and 30.0 ± 5.3% respectively, and there was no statistical significance between these rices. Additionally, in the Bt-T and Dongjin cultivars, the total lifespans from egg to adult were 39.5 ± 6.9 and 40.0 ± 5.8 days, and the weights of adult females were 1.78 ± 0.14 and 1.72 ± 0.16 mg, respectively. Therefore, there was no statistical difference in the biological parameters between these two varieties. Overall, the results indicate that the insect-resistant transgenic rice (Bt-T) did not negatively affect the reproduction and life cycle of brown planthopper, a non-target organism.

Keywords

Acknowledgement

본 연구는 농촌진흥청 연구개발사업(과제번호: PJ015745, PJ014235)의 지원으로 수행되었습니다.

References

  1. Amin MR, Oh SD, Bae EJ, Park SY, Suh SJ. 2020a. Impact of insect-resistant transgenic rice on above-ground non-target arthropods in Korea. Entomological Research 50:525-538. https://doi.org/10.1111/1748-5967.12473
  2. Amin MR, Oh SD, Suh SJ. 2020b. Comparing the effects of GM and non-GM soybean varieties on non-target arthropods. Entomological Research 50:423-432. https://doi.org/10.1111/1748-5967.12461
  3. Bae SD, Song YH, Park YD. 1987. Effects of temperature conditions on the growth and oviposition of brown planthopper, Nilaparvata lugens Stal. Korean Journal of Plant Protection 26:13-23. [in Korean]
  4. Bernal CC, Aguda RM, Cohen MB. 2002. Effect of rice lines transformed with Bacillus thuringiensis toxin genes on the brown planthopper and its predator Cyrtorhinus lividipennis. Entomologia Experimentalis et Applicata 102:21-28. https://doi.org/10.1046/j.1570-7458.2002.00921.x
  5. Chang X, Sun L, Ning D, Dang C, Yao H, Fang Q, Peng Y, Wang F, Ye G. 2020. Cry1C rice doesn't affect the ecological fitness of rice brown planthopper, Nilaparvata lugens either under RDV stress or not. Scientific Reports 10:16423. https://doi.org/10.1038/s41598-020-73465-7
  6. Cho JI, Park SH, Lee GS, Kim SM, Lim SM, Kim YS, Park SC. 2020. Current status of GM crop development and commercialization. Korean Journal of Breeding Science Special Issue 52:40-48. [in Korean] https://doi.org/10.9787/KJBS.2020.52.S.40
  7. Choi NJ, Jeong IH, Kwon DH, Choi MY, Baik CH. 2017. Life table analysis of the brown planthopper, Nilaparvata lugens Stal (Hemiptera: Delphacidae) on rice of resistant cultivars. Korean Journal of Environmental Biology 35:526-532. [in Korean] https://doi.org/10.11626/KJEB.2017.35.4.526
  8. Choi WS, Ahn SJ, Yoon JH, Kim HH, Jang JW, Park JJ. 2015. Comparing of insect fauna between transgenic rice and common rice cultivar based on light tap and sweeping methods. Journal of Agriculture & Life Science 49:1-17. [in Korean]
  9. Claus D, Berkeley RCW. 1986. Genus Bacillus Cohn 1872. In Bergey's Manual of Systematic Bacteriology. Vol. 2. pp. 1104-1139. The Williams & Wilkins Co., Baltimore, USA.
  10. Dang C, Sun C, Lu Z, Zhong F, Wang F, Wang Q, Sun R, Peng Y, Ye G. 2019. Cry2A rice did not affect the interspecific interactions between two rice planthoppers, Nilaparvata lugens, and Sogatella furcifera. GM Crops & Food 10:170-180. https://doi.org/10.1080/21645698.2019.1649530
  11. Dyck VA, Thomas B. 1979. The brown planthopper problem. pp. 3-17. In International Rice Research Institute (Ed.), Brown Planthopper: Threat to Rice Production in Asia. International Rice Research Institute, Los Banos, Philippines.
  12. Hu LX, Chi H, Zhang J, Zhou Q, Zhang RJ. 2010. Life-table analysis of the performance of Nilaparvata lugens (Hemiptera: Delphacidae) on two wild rice species. Journal of Economic Entomology 103:1628-1635. https://doi.org/10.1603/EC10058
  13. ISAAA (International Service for the Acquisition of Agri-biotech Applications). 2020. Global status of commercialized biotech/GM crops:2019, Brief No. 55-2019. ISAAA, New York, USA.
  14. Kim DY, Eom MS, Kim HJ, Ko EM, Pack IS, Park JH, Park KW, Nam KH, Oh SD, Kim JK, et al. 2020. Gene flow from transgenic soybean, developed to obtain recombinant proteins for use in the skin care industry, to non-transgenic soybean. Applied Biological Chemistry 63:65. https://doi.org/10.1186/s13765-020-00550-w
  15. Kim MK, Cohen MB, Roh JH, Kim YH, Im DJ, Hur IB, Chung DH, Kim KH. 1998. Reactions of resistance to brown planthopper in Japonica rice cultivars. RDA Journal of Crop Protection 40:10-15. [in Korean]
  16. Kim WJ, Park HS, Kim HS, Ha KY, Cho YC, Lee JH, Kim BK. 2016. Response to brown planthopper resistance genes at rice seedling stage. Korean Journal of Breeding Science 48:29-36. [in Korean] https://doi.org/10.9787/KJBS.2016.48.1.029
  17. KRIBB (Korea Research Institute of Bioscience and Biotechnology). 2019. Biosafety white paper 2019. KRIBB, Daejeon, Korea. [in Korean]
  18. Lee SY, Yeo YS, Park SY, Oh SW, Yoon EK, Shin KS, Woo HJ, Lim MH. 2015. Composition analysis of herbicide tolerant Ab rice and insect-resistant Bt rice. Korean Journal of Breeding Science 47:255-263. [in Korean] https://doi.org/10.9787/KJBS.2015.47.3.255
  19. Lim MH. 2018. Development of an insect-resistant rice with mcry1Ac1 gene for substantial equivalence assessment. Ph.D. dissertation, Kyungpook National Univ., Daegu, Korea. [in Korean]
  20. Lu ZB, Liu YE, Han NS, Tian JC, Peng YF, Hu C, Guo YY, Ye GY. 2014. Transgenic cry1C or cry2A rice has no adverse impacts on the life-table parameters and population dynamics of the brown planthopper, Nilaparvata lugens (Hemiptera Delphacidae). Pest Management Science 71:937-945. https://doi.org/10.1002/ps.3866
  21. Mannakkara A, Niu L, Ma WH, Lei CL. 2013. Zero effect of Bt rice on expression of genes coding for digestion, detoxification and immune responses and developmental performances of brown planthopper Nilaparvata lugens (Stal). Journal of Insect Physiology 59:985-993. https://doi.org/10.1016/j.jinsphys.2013.07.009
  22. Oh SD, Min SK, Kim JK, Park JH, Kim CG, Park SY. 2020. Evaluation of the acute toxicity of theoredoxin (TRX) transgenic soybean to Daphnia magna. Korean Journal of Agricultural Science 47:791-802. [in Korean] https://doi.org/10.7744/KJOAS.20200066
  23. Oh SW, Lee SM, Park SY, Lee SY, Lee WH, Cho HS, Yeo YS. 2016. Rice biotechnology and current development. Journal of the Korean Society of International Agriculture 28:24-36. [in Korean] https://doi.org/10.12719/KSIA.2016.28.1.24
  24. Park HH, Park CG, Park HM, Uhm KB. 2006. Rearing system for rice leaffolder, Cnapholocrocis medinalis (Lepidoptera: Cramdidae) using corn seedlings. Korean Journal of Applied Entomology 45:91-95.
  25. Rheey SH. 2019. A philosophical analysis of the issues of GM crops. Sogang Journal of Philosophy 56:9-31. [in Korean] https://doi.org/10.17325/sgjp.2019.56..9
  26. Song YH, Choi SY, Park JS. 1972. Studies on the resistance of "Tong-il" variety (IR-667) to brown planthopper, Nilaparvata lugens STAL. Korean Journal of Plant Protection 11:61-68. [in Korean]
  27. Yi HB, Kim HJ, Na SM. 2016. The methodology for environmental risk assessments of non-target organisms (insects) on LM rice. Korean Journal of Environment and Ecology 30:710-723. [in Korean]