한국자기공명학회논문지 (Journal of the Korean Magnetic Resonance Society)

한국자기공명학회 (Korean Magnetic Resonance Society)
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A practical approach to handling protein samples under degradation

  • Jeong-Yong, Suh (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Sung Hyun, Hong (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University)
  • 투고 : 2022.12.13
  • 심사 : 2022.12.18
  • 발행 : 2022.12.20
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초록

Protein structure determination using NMR spectroscopy requires a suite of heteronuclear 3-D NMR experiments that can take a couple of weeks for completion. During the experiments, protein samples may suffer from slow degradation due to co-purifying proteases, which complicates and slows down the assignment procedure. Here we describe a practical protocol to avoid unwanted proteolysis during the experiment.

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과제정보

This work was supported by Cooperative Research Program for Agriculture Science & Technology Development, Rural Development Administration (PJ011112016). The authors thank the high field NMR facility at the Korea Basic Science Institute and the National Center for Inter-university Research Facilities at Seoul National University.

참고문헌

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