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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Elevated plasma α1-antichymotrypsin is a biomarker candidate for malaria patients

  • Young Yil, Bahk (Department of Biotechnology, College of Biomedical and Health Science, Konkuk University) ;
  • Sang Bong, Lee (Department of Biotechnology, College of Biomedical and Health Science, Konkuk University) ;
  • Jong Bo, Kim (Department of Biotechnology, College of Biomedical and Health Science, Konkuk University) ;
  • Tong-Soo, Kim (Convergence Research Center for Insect Vectors, Incheon National University) ;
  • Sung-Jong, Hong (Convergence Research Center for Insect Vectors, Incheon National University) ;
  • Dong Min, Kim (Department of Internal Medicine, College of Medicine, Chosun University) ;
  • Sungkeun, Lee (Department of Pharmacology, Inha University School of Medicine)
  • Received : 2022.08.26
  • Accepted : 2022.09.25
  • Published : 2022.11.30
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Abstract

Advancements in the field of proteomics have provided opportunities to develop diagnostic and therapeutic strategies against various diseases. About half of the world's population remains at risk of malaria. Caused by protozoan parasites of the genus Plasmodium, malaria is one of the oldest and largest risk factors responsible for the global burden of infectious diseases with an estimated 3.2 billion persons at risk of infection. For epidemiological surveillance and appropriate treatment of individuals infected with Plasmodium spp., timely detection is critical. In this study, we used combinations of depletion of abundant plasma proteins, 2-dimensional gel electrophoresis (2-DE), image analysis, LC-MS/MS and western blot analysis on the plasma of healthy donors (100 individuals) and vivax and falciparum malaria patients (100 vivax malaria patients and 8 falciparum malaria patients). These analyses revealed that α1-antichymotrypsin (AACT) protein levels were elevated in vivax malaria patient plasma samples (mean fold-change ± standard error: 2.83 ± 0.11, based on band intensities), but not in plasma from patients with other mosquito-borne infectious diseases. The results of AACT immunoblot analyses showed that AACT protein was significantly elevated in vivax and falciparum malaria patient plasma samples (≥ 2-fold) compared to healthy control donor plasma samples, which has not been previously reported.

Keywords

Acknowledgement

This study was supported by funding from the National Research Fund (NRF-020R1F1A1070882 and NRF-2022R1F1A1066481) in the Republic of Korea.

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