Animal Bioscience

  • 제35권2호
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  • Pages.155-165
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  • 2022
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  • 2765-0189(pISSN)
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  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Glutamic-oxaloacetic transaminase 1 regulates adipocyte differentiation by altering nicotinamide adenine dinucleotide phosphate content

  • Yang, Yang (College of Animal Science, Shanxi Agricultural University) ;
  • Cheng, Zhimin (College of Animal Science, Shanxi Agricultural University) ;
  • Zhang, Wanfeng (College of Animal Science, Shanxi Agricultural University) ;
  • Hei, Wei (College of Animal Science, Shanxi Agricultural University) ;
  • Lu, Chang (College of Animal Science, Shanxi Agricultural University) ;
  • Cai, Chunbo (College of Animal Science, Shanxi Agricultural University) ;
  • Zhao, Yan (College of Animal Science, Shanxi Agricultural University) ;
  • Gao, Pengfei (College of Animal Science, Shanxi Agricultural University) ;
  • Guo, Xiaohong (College of Animal Science, Shanxi Agricultural University) ;
  • Cao, Guoqing (College of Animal Science, Shanxi Agricultural University) ;
  • Li, Bugao (College of Animal Science, Shanxi Agricultural University)
  • 투고 : 2021.04.14
  • 심사 : 2021.07.27
  • 발행 : 2022.02.01
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초록

Objective: This study was performed to examine whether the porcine glutamic-oxaloacetic transaminase 1 (GOT1) gene has important functions in regulating adipocyte differentiation. Methods: Porcine GOT1 knockout and overexpression vectors were constructed and transfected into the mouse adipogenic 3T3-L1 cells. Lipid droplets levels were measured after 8 days of differentiation. The mechanisms through which GOT1 participated in lipid deposition were examined by measuring the expression of malate dehydrogenase 1 (MDH1) and malic enzyme (ME1) and the cellular nicotinamide adenine dinucleotide phosphate (NADPH) content. Results: GOT1 knockout significantly decreased lipid deposition in the 3T3-L1 cells (p<0.01), whereas GOT1 overexpression significantly increased lipid accumulation (p<0.01). At the same time, GOT1 knockout significantly decreased the NADPH content and the expression of MDH1 and ME1 in the 3T3-L1 cells. Overexpression of GOT1 significantly increased the NADPH content and the expression of MDH1 and ME1, suggesting that GOT1 regulated adipocyte differentiation by altering the NADPH content. Conclusion: The results preliminarily revealed the effector mechanisms of GOT1 in regulating adipose differentiation. Thus, a theoretical basis is provided for improving the quality of pork and studies on diseases associated with lipid metabolism.

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과제정보

This work was supported by National Natural Science Foundation of China (31872336), Special Funds for Scholars Support Program of Shanxi Province (2016; 2017), Basic Research Project of Shanxi Province (201901D211376; 201901D211369).

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