Korean Journal of Exercise Nutrition (운동영양학회지)

한국운동영양학회
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Relationship between plasma asymmetric dimethylarginine and nitric oxide levels afects aerobic exercise training-induced reduction of arterial stifness in middle-aged and older adults

  • Shimomura, Mio (Faculty of Sport and Health Science, Ritsumeikan University) ;
  • Fujie, Shumpei (Faculty of Sport and Health Science, Ritsumeikan University) ;
  • Sanada, Kiyoshi (Faculty of Sport and Health Science, Ritsumeikan University) ;
  • Kajimoto, Hiroki (Faculty of Sport and Health Science, Ritsumeikan University) ;
  • Hamaoka, Takafumi (Sports Medicine for Health Promotion, Tokyo Medical University) ;
  • Iemitsu, Motoyuki (Faculty of Sport and Health Science, Ritsumeikan University)
  • Received : 2021.01.29
  • Accepted : 2021.03.11
  • Published : 2021.03.31
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Abstract

[Purpose] Aerobic exercise training (AT) reverses aging-induced deterioration of arterial stiffness via increased arterial nitric oxide (NO) production. Asymmetric dimethylarginine (ADMA), an endogenous inhibitor of NO synthase, was decreased by AT. However, whether AT-induced changes in ADMA levels are related to changes in nitrite/nitrate (NOx) levels remains unclear. Accordingly, we aimed to clarify whether the relationship between plasma ADMA and NOx levels afected the AT-induced reduction of arterial stifness in middle-aged and older adults. [Methods] Thirty-one healthy middle-aged and older male and female subjects (66.4 ± 1.3 years) were randomly divided into two groups: exercise intervention and sedentary controls. Subjects in the training group completed an 8-week AT (60%-70% peak oxygen uptake [${\dot{V}}O_{2peak}$] for 45 min, 3 days/week). [Results] AT signifcantly increased ${\dot{V}}O_{2peak}$ (P < 0.05) and decreased carotid β-stifness (P < 0.01). Moreover, plasma ADMA levels were significantly decreased while plasma NOx levels and NOx/ADMA ratio were significantly increased by AT (P < 0.01). Additionally, no sex diferences in AT-induced changes of circulating ADMA and NOx levels, NOx/ADMA ratio, and carotid β-stifness were observed. Furthermore, the AT-induced increase in circulating ADMA levels was negatively correlated with an increase in circulating NOx levels (r = -0.414, P < 0.05), and the AT-induced increase in NOx/ADMA ratio was negatively correlated with a decrease in carotid β-stifness (r = -0.514, P < 0.01). [Conclusion] These results suggest that the increase in circulating NOx with reduction of ADMA elicited by AT is associated with a decrease in arterial stiffness regardless of sex in middle-aged and older adults.

Keywords

Acknowledgement

The authors declare no conflicts of interest. This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (#19K22828, M. Iemitsu).

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