Korean Journal of Exercise Nutrition (운동영양학회지)

한국운동영양학회
권호 표지
DOI QR코드

DOI QR Code

Voluntary exercise training improves body weight of leptin-deficient ob/ob mice by altering hepatic stearoyl-CoA desaturase 1 and deleted in breast cancer 1 protein levels

  • Lim, Wonchung (Department of Sports Medicine, Cheongju University) ;
  • Hwang, Moon-Hyon (Division of Health & Kinesiology, Incheon National University) ;
  • Kang, Chounghun (Department of Physical Education, Inha University) ;
  • Kim, So Yeon (Department of Dental Hygiene, Cheongju University) ;
  • Cho, Hyeseong (Department of Biochemistry and Molecular Biology, Ajou University School of Medicine)
  • Received : 2021.11.11
  • Accepted : 2021.12.30
  • Published : 2021.12.31
⟨ Previous Next ⟩

Abstract

[Purpose] Deleted in breast cancer 1 (DBC1) ablation causes obesity, and stearoyl-CoA desaturase 1 (SCD1) induces the biosynthesis of monounsaturated fatty acids. This study examined whether voluntary wheel running (VWR) alters SCD-1 and DBC1 protein levels in the liver of leptin-deficient ob/ob mice. [Methods] Twenty-five Ob/Ob mice were divided into two groups (ob/ob-Sed and ob/ob-Ex). The expression of DBC1 and SCD1 in the mouse liver was determined using western blotting. [Results] After 10 weeks, VWR significantly reduced body weight without affecting the fatty acid synthase and CD36 protein levels. The average daily running distance was 4.0±1.0 km/day. This improvement was associated with changes in the hepatic SCD1 and DBC1 levels. Hepatic SCD-1 protein levels increased significantly, and DBC1 protein levels decreased in ob/ob-Sed animals. On the other hand, VWR inhibited the obesity-induced increase in SCD1 expression and impaired the obesity-induced decrease in DBC1 expression in the liver of leptin-deficient ob/ob mice. [Conclusion] This is the first study showing that VWR has strong effects on hepatic SCD1 and DBC1 in ob/ob mice, and provides key insights into the effects of exercise on obesity.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF-2018R1D1A1B07048750 and NRF-2019S1A5A2A03051702) funded by the Korean government.

References

  1. Petridou A, Siopi A, Mougios V. Exercise in the management of obesity. Metabolism. 2019;92:163-9. https://doi.org/10.1016/j.metabol.2018.10.009
  2. Azuma K, Heilbronn LK, Albu JB, Smith SR, Ravussin E, Kelley DE, Look AHEAD Adipose Research Group. Adipose tissue distribution in relation to insulin resistance in type 2 diabetes mellitus. Am J Physiol Endocrinol Metab. 2007;293:E435-42. https://doi.org/10.1152/ajpendo.00394.2006
  3. Bray GA, Jablonski KA, Fujimoto WY, Barrett-Connor E, Haffner S, Hanson RL, Hill JO, Hubbard V, Kriska A, Stamm E, Pi-Sunyer FX, Diabetes Prevention Program Research Group. Relation of central adiposity and body mass index to the development of diabetes in the diabetes prevention program. Am J Clin Nutr. 2008;87:1212-8. https://doi.org/10.1093/ajcn/87.5.1212
  4. Quiroga AD, Lehner R. Liver triacylglycerol lipases. Biochim Biophys Acta. 2012;1821:762-9. https://doi.org/10.1016/j.bbalip.2011.09.007
  5. Dobrzyn P, Ntambi JM, Dobrzyn A. Stearoyl-CoA desaturase: a novel control pint of lipid metabolism and insulin sensitivity. Eur J Lipid Sci Technol. 2008;110:93-100. https://doi.org/10.1002/ejlt.200700249
  6. Ntambi JM, Miyazaki M, Stoehr JP, Lan H, Kendziorski CM, Yandell BS, Song Y, Cohen P, Friedman JM, Attie AD. Loss of stearoyl-CoA desaturase-1 function protects mice against adiposity. Proc Natl Acad Sci USA. 2002;99:11482-6. https://doi.org/10.1073/pnas.132384699
  7. Cohen P, Miyazaki M, Socci ND, Hagge-Greenberg A, Liedtke W, Soukas AA, Sharma R, Hudgins LC, Ntambi JM, Friedman JM. Role for stearoyl-CoA desaturase-1 in leptin-mediated weight loss. Science. 2002;297:240-3. https://doi.org/10.1126/science.1071527
  8. Hawley JA, Hargreaves M, Joyner MJ, Zierath JR. Integrative biology of exercise. Cell. 2014;159:738-49. https://doi.org/10.1016/j.cell.2014.10.029
  9. O'Gorman DJ, Krook A. Exercise and the treatment of diabetes and obesity. Endocrinol Metab Clin North Am. 2008;37:887-903. https://doi.org/10.1016/j.ecl.2008.07.006
  10. Rogowski MP, Flowers MT, Stamatikos AD, Ntambi JM, Paton CM. SCD1 activity in muscle increases triglyceride PUFA content, exercise capacity, and PPARδ expression in mice. J Lipid Res. 2013;54:2636-46. https://doi.org/10.1194/jlr.M035865
  11. Yasari S, Prud'homme D, Wang D, Jankowski M, Levy E, Gutkowska J, Lavoie JM. Exercise training decreases hepatic SCD-1 gene expression and protein content in rats. Mol Cell Biochem. 2010;335:291-9. https://doi.org/10.1007/s11010-009-0279-y
  12. Zhu X, Bian H, Wang L, Sun X, Xu X, Yan H, Xia M, Chang X, Lu Y, Li Y, Xia P, Li X, Gao X. Berberine attenuates nonalcoholic hepatic steatosis through the ampk-srebp-1c-SCD1 pathway. Free Radic Biol Med. 2019;141:192-204. https://doi.org/10.1016/j.freeradbiomed.2019.06.019
  13. Ortinau LC, Pickering RT, Nickelson KJ, Stromsdorfer KL, Naik CY, Haynes RA, Bauman DE, Rector RS, Fritsche KL, Perfield JW 2nd. Sterculic oil, a natural SCD1 inhibitor, improves glucose tolerance in obese ob/ob Mice. ISRN Endocrinol. 2012;2012:947323. https://doi.org/10.5402/2012/947323
  14. Escande C, Nin V, Pirtskhalava T, Chini CC, Tchkonia T, Kirkland JL, Chini EN. Deleted in breast cancer 1 limits adipose tissue fat accumulation and plays a key role in the development of metabolic syndrome phenotype. Diabetes. 2015;64:12-22. https://doi.org/10.2337/db14-0192
  15. Qiang L, Kon N, Zhao W, Jiang L, Knight CM, Welch C, Pajvani U, Gu W, Accili D. Hepatic sirT1-dependent gain of function of stearoyl-coa desaturase-1 conveys dysmetabolic and tumor progression functions. Cell Rep. 2015;11:1797-808. https://doi.org/10.1016/j.celrep.2015.05.025
  16. Baldini SF, Wavelet C, Hainault I, Guinez C, Lefebvre T. The nutrient-dependent O-GlcNAc modification controls the expression of liver fatty acid synthase. J Mol Biol. 2016;428:3295-304. https://doi.org/10.1016/j.jmb.2016.04.035
  17. Su T, Huang C, Yang C, Jiang T, Su J, Chen M, Fatima S, Gong R, Hu X, Bian Z, Liu Z, Kwan HY. Apigenin inhibits STAT3/CD36 signaling axis and reduces visceral obesity. Pharmacol Res. 2020;152:104586. https://doi.org/10.1016/j.phrs.2019.104586
  18. Despres JP, Lamarche B. Low-intensity endurance exercise training, plasma lipoproteins and the risk of coronary heart disease. J Intern Med. 1994;236:7-22. https://doi.org/10.1111/j.1365-2796.1994.tb01114.x
  19. Boule NG, Haddad E, Kenny GP, Wells GA, Sigal RJ. Effects of exercise on glycemic control and body mass in type 2 diabetes mellitus: a meta-analysis of controlled clinical trials. JAMA. 2001;286:1218-27. https://doi.org/10.1001/jama.286.10.1218
  20. Gioscia-Ryan RA, Clayton ZS, Zigler MC, Richey JJ, Cuevas LM, Rossman MJ, Battson ML, Ziemba BP, Hutton DA, VanDongen NS, Seals DR. Lifelong voluntary aerobic exercise prevents age- and Western diet-induced vascular dysfunction, mitochondrial oxidative stress and inflammation in mice. J Physiol. 2021;599:911-25. https://doi.org/10.1113/JP280607
  21. Zhang W, Yang B, Weng H, Liu T, Shi L, Yu P, So KF, Qu Y, Zhou L. Wheel running improves motor function and spinal cord plasticity in mice with genetic absence of the corticospinal tract. Front Cell Neurosci. 2019;13:106. https://doi.org/10.3389/fncel.2019.00106
  22. Roden M, Ludwig C, Nowotny P, Schneider B, Clodi M, Vierhapper H, Roden A, Waldhausl W. Relative hypoleptinemia in patients with type 1 and type 2 diabetes mellitus. Int J Obes Relat Metab Disord. 2000;24:976-81. https://doi.org/10.1038/sj.ijo.0801266
  23. Zhang M, Sun W, Zhou M, Tang Y. MicroRNA-27a regulates hepatic lipid metabolism and alleviates NAFLD via repressing FAS and SCD1. Sci Rep. 2017;7:14493-502. https://doi.org/10.1038/s41598-017-15141-x