Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Flow cytometric evaluation of the potential of metal oxide nanoparticles for skin sensitization using 5-Bromo-2-deoxyuridine

  • Lee, Dong Han (Division of Toxicological Research, Ministry of Food and Drug Safety, National Institute of Food and Drug Safety Evaluation) ;
  • Kim, Sung-Hyun (Division of Toxicological Research, Ministry of Food and Drug Safety, National Institute of Food and Drug Safety Evaluation) ;
  • Lee, Jin Hee (Division of Toxicological Research, Ministry of Food and Drug Safety, National Institute of Food and Drug Safety Evaluation) ;
  • Yang, Jun-Young (Division of Toxicological Research, Ministry of Food and Drug Safety, National Institute of Food and Drug Safety Evaluation) ;
  • Seok, Ji-Hyun (Division of Toxicological Research, Ministry of Food and Drug Safety, National Institute of Food and Drug Safety Evaluation) ;
  • Jung, Kikyung (Division of Toxicological Research, Ministry of Food and Drug Safety, National Institute of Food and Drug Safety Evaluation) ;
  • Lee, Jong Kwon (Division of Toxicological Research, Ministry of Food and Drug Safety, National Institute of Food and Drug Safety Evaluation)
  • Received : 2020.04.05
  • Accepted : 2020.11.04
  • Published : 2021.07.15
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Abstract

Although skin sensitization potential of various chemicals has been extensively studied, there are only a few reports on nanoparticles induced skin sensitization. Aiming to fill this lacuna, in this study we evaluated the potential of metal oxide nanoparticles (NPs) to induce skin sensitization with flow cytometry. Seven different metal oxide NPs, including copper oxide, cobalt oxide, nickel oxide, titanium oxide, cerium oxide, iron oxide, and zinc oxide were applied to Balb/c mice. After selecting the proper vehicle, the NPs were applied, and the skin sensitization potential were assessed using 5-bromo-2-deoxyuridine with flow cytometry. Physiochemical properties such as hydrodynamic size, polydispersity, and zeta potential were measured for the NPs prior to the tests. All the seven metal oxide NPs studied showed negative responses for skin sensitization potential. These results suggest that the OECD TG 442B using 5-bromo-2-deoxyuridine with flow cytometry can be applied to evaluate the potential of NPs for skin sensitization.

Keywords

Acknowledgement

This work was supported by the Korean Ministry of Food and Drug Safety [Grant Numbers 18181MFDS361 (2018, 2019)]. We would like to thank Editage (www.editage.co.kr) for English language editing.

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