Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Testosterone recuperates deteriorated male fertility in cypermethrin intoxicated rats

  • Katragadda, Vasudha (Department of Biotechnology, Sri Padmavati Mahila Visvavidyalayam (Women's University)) ;
  • Adem, Meghapriya (Department of Biotechnology, Sri Padmavati Mahila Visvavidyalayam (Women's University)) ;
  • Mohammad, Reshma Anjum (Department of Biotechnology, Sri Padmavati Mahila Visvavidyalayam (Women's University)) ;
  • Bhasyam, Sainath Sri (Department of Biotechnology, Vikrama Simhapuri University) ;
  • Battini, Kishori (Department of Biotechnology, Sri Padmavati Mahila Visvavidyalayam (Women's University))
  • Received : 2019.10.07
  • Accepted : 2020.03.26
  • Published : 2021.01.15
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Abstract

The present study investigates the protective effects of testosterone against reproductive toxicity induced by cypermethrin (50 mg/kg body weight) in rats. Significant reduction in the testicular and accessory sex organ weights were observed in cypermethrin-treated rats over controls. Cypermethrin intoxication significantly reduced testicular daily sperm count, epididymal sperm count, sperm motility, sperm viability and HOS-tail coiled sperm accompanied by significant reduction in the activity levels of testicular steroidogenic enzymes such as 3β- and 17β- hydroxysteroid dehydrogenases in rats as compared to controls. Further, qPCR studies indicated that the mRNA expression levels of steroidogenic acute regulatory protein (StAR) significantly decreased in cypermethrin-treated rats over controls. Molecular docking analysis indicated that the binding affinity of cypermethrin (-11.2 kcal/mol) towards StAR protein was greater as compared to its natural ligand, cholesterol (-8.2 kcal/mol) suggesting improper cholesterol channeling across the testis. Significant reduction in the circulatory levels of testosterone was also recorded in cypermethrin-exposed rats. An increase in pre- and post-implantation loss was observed in rats cohabited with cypermethrin-treated rats. On the other hand, testosterone (4.16 mg/kg body weight) treatment ameliorated cypermethrin-induced reprotoxic effects in rats. To conclude, cypermethrin-induced deterioration of suppressed reproductive performance in male rats could be linked to its antiandrogenic effects and on the other hand, testosterone-mediated protection of male reproductive health in cypermethrin-treated rats at least in part occurs via restoration of testosterone biosynthesis, spermatogenesis and sperm maturation events.

Keywords

Acknowledgement

The authors are thankful to Head, Department of Biotechnology, Sri Padmavati Mahila Visvavidyalayam, Tirupati for providing laboratory facilities. Authors also thankful to DST-CURIE, Central Instrumentation Facility, Sri Padmavati Mahila Visvavidyalayam (Women's University), Tirupati, for permitting to utilize equipments.

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