Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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The evaluation of Anti-oxidation, Anti-inflammation and Anti-wrinkle activity of Jeju Native Achyranthes japonica Nakai

제주 자생 우슬(Achyranthes japonica Nakai)의 항산화, 항염증과 항주름 평가

  • Um, Mi-Sun (Department of Beauty Care, Jangan University)
  • 엄미선 (장안대학교 뷰티케어과)
  • Received : 2021.08.29
  • Accepted : 2021.10.25
  • Published : 2021.10.30
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Abstract

The aim of this study was to assure the possiblility of Achyranthes japonica Nakai for a cosmetic material. For this we examined the Anti-oxidation, Anti-inflammation, Anti-wrinkle effects of Achyranthes japonica Nakai extract. Each material is extracted from Achyranthes japonica Nakai roots(AJNR) and Achyranthes japonica Nakai stalks(AJNS) using 70% ethanol. We carried out Nitric oxide assay of the extract with cultured RAW 264.7 macrophage cells, And we conducted MMP-1 assay, Type I procollagen synthesis assay of the extract with cultured CCD-986sk fibroblast cells. After we measured ABTS+ assay and SOD assay of AJNR and AJNS, we knew that effect of anti-oxidant increased as their concentration get higher. The result of this study was that the antioxidant activity of AJNR and AJNS was similarly excellent and the anti-inflammatory activity of AJNR was significantly higher than AJNS. The MMP-1 inhibitory activity and Type I procollagen synthesis of AJNR and AJNS was generally excellent, AJNS had slightly better than AJNR. Therefore I thought it has the chance to play an imfortant role as a functional cosmetic material for anti-oxidant, anti-inflammation and wrinkle reduction effects.

본 연구는 화장품 소재로서 우슬(Achyranthes japonica Nakai)의 가능성을 확인하기 위한 것이다. 우슬 추출물이 가지고 있는 항산화, 항염증, 항주름 효과를 측정하였다. 각각의 식물 재료는 70% 에탄올을 이용하여 우슬 뿌리(Achyranthes japonica Nakai roots, AJNR)와 우슬 줄기(Achyranthes japonica Nakai stalks, AJNS)로부터 추출하였다. RAW 264.7 세포를 배양하여 추출물의 Nitric oxide assay를 진행하였고, 섬유아세포 CCD-986sk를 배양하여 추출물의 MMP-1 assay, Type I procollagen synthesis assay를 실시하였다. 이 연구의 결과, 항산화 활성은 우슬 뿌리와 우슬 줄기 모두 우수하였고, 뿌리는 함염증 효과가 월등하게 우수하였으며 줄기는 뿌리에 비해 MMP-1 저해 활성과 Type I procollagen 합성 효과가 조금 더 높았다. 이 연구의 결과, 우슬 뿌리와 우슬 줄기의 항산화 활성은 유사한 수준으로 우수하였고, 뿌리의 항염 활성은 줄기보다 월등하게 높았다. MMP-1 저해 활성과 Type I procollagen 합성 효과는 대체적으로 우수하였는데 줄기가 뿌리에 비해 가 조금 더 뛰어났다. 그러므로 우슬은 항산화, 항염증, 항주름의 활성을 갖는 기능성 화장품 소재로서 활용이 가능할 것으로 판단된다.

Keywords

Acknowledgement

이 논문은 장안대학교 2021년도 연구비 지원에 의하여 연구된 것으로 이에 감사드립니다.

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