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생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Visualization of chromatin higher-order structures and dynamics in live cells

  • Park, Tae Lim (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST)) ;
  • Lee, YigJi (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST)) ;
  • Cho, Won-Ki (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST))
  • 투고 : 2021.07.16
  • 심사 : 2021.08.29
  • 발행 : 2021.10.31
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초록

Chromatin has highly organized structures in the nucleus, and these higher-order structures are proposed to regulate gene activities and cellular processes. Sequencing-based techniques, such as Hi-C, and fluorescent in situ hybridization (FISH) have revealed a spatial segregation of active and inactive compartments of chromatin, as well as the non-random positioning of chromosomes in the nucleus, respectively. However, regardless of their efficiency in capturing target genomic sites, these techniques are limited to fixed cells. Since chromatin has dynamic structures, live cell imaging techniques are highlighted for their ability to detect conformational changes in chromatin at a specific time point, or to track various arrangements of chromatin through long-term imaging. Given that the imaging approaches to study live cells are dramatically advanced, we recapitulate methods that are widely used to visualize the dynamics of higher-order chromatin structures.

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과제정보

This work was supported by the Suh Kyungbae Foundation and National Research Foundation of Korea (NRF) grants (2020R1C1C1014599, 2020R1A4A3079755, 2019M3A9H1103711, and 2019R1A6A1A10073887 to W.-K. Cho).

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