Current Optics and Photonics

Optical Society of Korea (한국광학회)
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Study of Optimal Conditions Affecting the Photothermal Effect and Fluorescence Characteristics of Indocyanine Green

  • Seo, Sung Hoon (Department of Physics and Optoelectronics Convergence Research Center, Chonnam National University) ;
  • Bae, Min Gyu (Department of Physics and Optoelectronics Convergence Research Center, Chonnam National University) ;
  • Park, Hyeong Ju (Medical Laser Research Center, Dankook University) ;
  • Ahn, Jae Sung (Medical & Bio Photonics Research Center, Korea Photonics Technology Institute) ;
  • Lee, Joong Wook (Department of Physics and Optoelectronics Convergence Research Center, Chonnam National University)
  • Received : 2021.05.27
  • Accepted : 2021.08.05
  • Published : 2021.10.25
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Abstract

Indocyanine green (ICG) is a cyanine dye that has been used in medical diagnostics based on fluorescence imaging, and in medical therapy based on the photothermal effect. It is important to systematically understand the photothermal effect and fluorescence characteristics of ICG simultaneously. By varying a number of conditions such as laser power density, laser irradiation wavelength, concentration of ICG solution, and exposure time of laser irradiation, the intensity properties of fluorescence and the temperature change induced by the photothermal effect are measured simultaneously using a charge-coupled-device camera and a thermal-imaging camera. The optimal conditions for maximizing the photothermal effect are determined, while maintaining a relatively long lifetime and high efficiency of the fluorescence for fluorescence imaging. When the concentration of ICG is approximately 50 ㎍/ml and the laser power density exceeds 1.5 W/cm2, the fluorescence lifetime is the longest and the temperature induced by the photothermal effect rapidly increases, exceeding the critical temperature sufficient to damage human cells and tissues. The findings provide useful insight into the realization of effective photothermal therapy, while also specifying the site to be treated and enabling real-time treatment monitoring.

Keywords

Acknowledgement

This research was funded by the Korea Electric Power Corporation (Grant Number: R18XA06-79), and was also partially supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (Grant Number: NRF-2017M3A9E2056373; NRF-2019R1F1A1058851).

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