The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Diversity and Mycotoxin Production of Aspergillus flavus in Stored Peanut

저장 땅콩에서 분리된 Aspergillus flavus의 다양성 및 독소생성능

  • Choi, Jung-Hye (Division of Microbial Safety, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Nah, Ju-Young (Division of Microbial Safety, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Lee, Mi-Jeong (Division of Microbial Safety, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Lim, Su-Bin (Division of Microbial Safety, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Lee, Theresa (Division of Microbial Safety, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Jeomsoon (Division of Microbial Safety, National Institute of Agricultural Sciences, Rural Development Administration)
  • 최정혜 (국립농업과학원 유해생물과) ;
  • 나주영 (국립농업과학원 유해생물과) ;
  • 이미정 (국립농업과학원 유해생물과) ;
  • 임수빈 (국립농업과학원 유해생물과) ;
  • 이데레사 (국립농업과학원 유해생물과) ;
  • 김점순 (국립농업과학원 유해생물과)
  • Received : 2021.07.13
  • Accepted : 2021.09.17
  • Published : 2021.09.30
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Abstract

Peanuts in storage were estimated for mycotoxigenic fungi and mycotoxins. Peanut samples collected from storages in Gochang were mainly contaminated with Fusarium (17.2±28.0%), Penicillium (12.4±28.0%), and Aspergillus (8.0±7.6%). Other genera, including Talaromyces, Rhizopus, Rhizoctonia, Trichocladium, Clonostachys, Mucor, Chaetomium, Trametes, Epicoccum, and Humicola, were also found. Although aflatoxins were not detected in the peanut samples, 29 strains of Aspergillus flavus were identified using molecular marker genes. Among them, 17 selected isolates produced aflatoxins in solid culture media ranging from 0.61-187.82 ㎍/kg. All of them could produce both aflatoxin B1 and B2 and some (n=5) produced additional G1, G2, or both. This study is the first report that A. flavus stains obtained from Korean stored peanut are aflatoxigenic.

땅콩의 독성곰팡이 및 곰팡이독소 오염현황을 조사하기 위해 고창 지역의 저장고에서 땅콩을 수집하였다. 수집된 땅콩은 Fusarium (17.2±28.0%), Penicillium (12.4±28.0%), Aspergillus (8.0±7.6%) 속 곰팡이로 주로 오염되어 있었으며, 그 외에 Talaromyces, Rhizopus, Rhizoctonia, Trichocladium, Clonostachys, Mucor, Chaetomium, Trametes, Epicoccum, Humicola 속 곰팡이가 검출되었다. 땅콩시료에서 아플라톡신은 검출되지 않았으나, 29점의A. flavus 균주가 분리, 동정되었다. 그 중 17점의 균주를 선발하여 potato dextrose agar (PDA) 배지 상에서 아플라톡신 생성능을 분석한 결과 0.61-187.82 ㎍/kg의 농도범위에서 모두 아플라톡신을 생성하였다. 이들 17 균주는 아플라톡신 B1, B2도 생성하였으며, 일부(5 균주)는 G1 또는 G2를 생성하였다. 이 연구는 국내 저장 땅콩에서A. flavus 오염률 및 독소생성능에 대한 첫 보고이다.

Keywords

Acknowledgement

본 연구는 농촌진흥청 국립농업과학원 농업과학기술 연구개발사업(과제번호: PJ014811)의 지원에 의해 수행된 결과로, 이에 감사드립니다.

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