DOI QR코드

DOI QR Code

Aqueous Extract of Lysimachia christinae Hance Prevents Cholesterol Gallstone in Mice by Affecting the Intestinal Microflora

  • Liu, Shijia (First Clinical College, Chongqing Medical University) ;
  • Luorong, Quji (College of Traditional Chinese Medicine, Chongqing Medical University) ;
  • Hu, Kaizhi (Chongqing Institute of Pharmaceutical Plant) ;
  • Cao, Weiguo (College of Traditional Chinese Medicine, Chongqing Medical University) ;
  • Tao, Wei (College of Traditional Chinese Medicine, Chongqing Medical University) ;
  • Liu, Handeng (Laboratory of Tissue and Cell Biology, Experimental Teaching Center, Chongqing Medical University) ;
  • Zhang, Dan (College of Traditional Chinese Medicine, Chongqing Medical University)
  • 투고 : 2021.06.15
  • 심사 : 2021.07.13
  • 발행 : 2021.09.28

초록

With changes in human dietary patterns, the proportion of high-fat and high-cholesterol foods in the daily diet has increased. As a result, the incidence rate of cholelithiasis is increasing rapidly. Many studies have reported on the crucial role that the intestinal microflora plays in the progression of gallstones. Although the whole herb of Lysimachia christinae, a traditional Chinese medicine, has long been extensively used as a remedy for cholelithiasis in China, its effects on the intestinal microflora remain unknown. Hence, in this study, we investigated the ability of the aqueous extract of L. christinae (LAE) to prevent cholesterol gallstones (CGSs) in model animals by affecting the intestinal microflora. The effects of LAE on body weight, serum lipid profile, visceral organ indexes, and histomorphology were studied in male C57BL/6J mice, which were induced by a lithogenic diet. After the 8-week study, CGSs formation was greatly reduced after LAE treatment. LAE also reduced body weight gain and hyperlipidemia and restored the histomorphological changes. Moreover, the intestinal microflora exhibited significant variation. In the model group fed the lithogenic diet, the abundances of the genera unclassified Porphyromonadaceae, Lactobacillus and Alloprevotella decreased, but in contrast, Akkermansia dramatically increased compared with the control check group, which was fed a normal diet; the administration of LAE reversed these changes. These results imply that L. christinae can be considered an efficient therapy for eliminating CGSs induced by a high-fat and high-cholesterol diet, which may be achieved by influencing the intestinal microflora.

키워드

과제정보

This research is funded by the Chongqing Technology Innovation and Application Demonstration Project (Grant No. cstc2017shmsA130014, cstc2018jscx-mszd0007 and cstc2019jscx-gksbX0010) and the Important Science and Technology Research Projects for Forestry of Chongqing (Grant No. 2018-3).

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