Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Morphology and Molecular Characteristics of Alternaria sonchi Causing Brown Leaf Spot on Sonchus asper in Korea

  • Luo, Huan (Department of Applied Biology, Chungnam National University) ;
  • Park, Myung Soo (School of Biological Sciences and Institute of Microbiology, Seoul National University) ;
  • Yu, Jun Myoung (Department of Applied Biology, Chungnam National University)
  • Received : 2021.07.20
  • Accepted : 2021.09.29
  • Published : 2021.09.30
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Abstract

During a disease survey on weeds and minor cultivated crops in Korea, a brown leaf spot disease was observed on Sonchus asper. Leaf lesions were round or irregular in shape, and grayish brown to brown with a purple margin. In severe infection, lesions enlarged and coalesced, resulting in blighting of the leaves. The isolates from these leaf lesions were identified as Alternaira sonchi based on morphological characteristics and phylogenetic analyses of Internal transcribed spacer region, Alternaria allergen a1, glyceraldehyde 3-phosphate dehydrogenase, RNA polymerase II, and translation elongation factor genes. This study provides a comprehensive description of the morphological characteristics and phylogenetical traits of A. sonchi causing brown leaf spot on S. asper in Korea.

Keywords

Acknowledgement

This project was support by the "Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ014507022021)" Rural Development Administration, Republic of Korea.

References

  1. Akhtar, K. P. 2019. Alternaria alternata and Alternaria sonchi: two new records of fungal pathogens on Sonchus asper (annual sow thistle) in Pakistan. Plant Prot. 3: 101-104. https://doi.org/10.33804/pp.003.02.0119
  2. Auld, B. A., Mead, E. and Medd, R. W. 1987. Weeds: An Illustrated Botanical Guide to the Weeds of Australia. Inkata Press, Melbourne, Austrailia. 255 pp.
  3. Berbee, M. L., Pirseyedi, M. and Hubbard, S. 1999. Cochliobolus phylogenetics and the origin of known, highly virulent pathogens, inferred from ITS and glyceraldehyde-3-phosphate dehydrogenase gene sequences. Mycologia 91: 964-977. https://doi.org/10.2307/3761627
  4. Brooker, S. G., Cambie, R. C. and Cooper, R. C. 1989. Economic native plants of New Zealand. Econ. Bot. 43: 79-106. https://doi.org/10.1007/BF02859329
  5. Carbone, I. and Kohn, L. M. 1999. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91: 553-556. https://doi.org/10.2307/3761358
  6. Chun, J. 1995. Computer assisted classification and identification of actinomycetes. Ph.D. thesis. Newcastle University, Newcastle upon Tyne, UK. 416 pp.
  7. Deng, J. X., Li, M. J., Paul, N. C., Lee, J. H., Cho, H. S., Lee, H. B. et al. 2015. Alternaria species associated with araliaceous plants in Korea. Mycol. Prog. 14: 31. https://doi.org/10.1007/s11557-015-1052-0
  8. Deng, J. X., Paul, N. C., Park, M. S. and Yu, S. H. 2013. Molecular characterization, morphology, and pathogenicity of Alternaria panax from araliaceous plants in Korea. Mycol. Prog. 12: 383-396. https://doi.org/10.1007/s11557-012-0844-8
  9. Elliott, J. A. 1916. An Alternaria on Sonchus. Bot. Gaz. 62: 414-416. https://doi.org/10.1086/331952
  10. Ellis, M. B. 1971. Dematiaceous Hyphomycetes. Commonwealth Mycological Institute, Kew, London, UK. 608 pp.
  11. Facciola, S. 1990. Cornucopia: A Source Book of Edible Plants. Kampong Publications, Vista, CA, USA. 677 pp.
  12. Gautam, A. K. and Avasthi, S. 2018. Diversity of powdery mildew fungi from North Western Himalayan region of Himachal Pradesh: a checklist. Plant Pathol. Quar. 8: 78-99. https://doi.org/10.5943/ppq/8/1/11
  13. Hong, S. G., Cramer, R. A., Lawrence, C. B. and Pryor, B. M. 2005. Alt a 1 allergen homologs from Alternaria and related taxa: analysis of phylogenetic content and secondary structure. Fungal Genet. Biol. 42: 119-129. https://doi.org/10.1016/j.fgb.2004.10.009
  14. Khan, R. A., Khan, M. R., Sahreen, S. and Bokhari, J. 2010. Antimicrobial and phytotoxic screening of various fractions of Sonchus asper. Afr. J. Biotechnol. 9: 3883-3887.
  15. Kumar, S., Tamura, K. and Nei, M. 1994. MEGA: molecular evolutionary genetics analysis software for microcomputers. Comput. Appl. Biosci. 10: 189-191.
  16. Lawrence, D. P., Gannibal, P. B., Peever, T. L. and Pryor, B. M. 2013. The sections of Alternaria: formalizing species-group concepts. Mycologia 105: 530-546. https://doi.org/10.3852/12-249
  17. Liu, Y. J., Whelen, S. and Hall, B. D. 1999. Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse II subunit. Mol. Biol. Evol. 16: 1799-1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
  18. Moerman, D. E. 1998. Native American Ethnobotany. Timber Press, Portland, OR, USA. 927 pp.
  19. National Institute of Agricultural Sciences and The Korea Society of Plant Pathology. 2021. List of Plant Diseases in Korea. 6th ed. URL http://genebank.rda.go.kr/kplantdisease.do [15 September 2021].
  20. O'Donnell, K., Kistler, H. C., Cigelnik, E. and Ploetz, R. C. 1998. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proc. Natl. Acad. Sci. U. S. A. 95: 2044-2049. https://doi.org/10.1073/pnas.95.5.2044
  21. Park, M. S., Romanoski, C. E. and Pryor, B. M. 2008. A re-examination of the phylogenetic relationship between the causal agents of carrot black rot, Alternaria radicina and A. carotiincultae. Mycologia 100: 511-527. https://doi.org/10.3852/07-186R1
  22. Park, M. S., Seo, G. S., Bae, K. S. and Yu, S. H. 2005. Characterization of Trichoderma spp. associated with green mold of oyster mushroom by PCR-RFLP and sequence analysis of ITS regions of rDNA. Plant Pathol. J. 21: 229-236. https://doi.org/10.5423/PPJ.2005.21.3.229
  23. Peever, T. L., Su, G., Carpenter-Boggs, L. and Timmer, L. W. 2004. Molecular systematics of citrus-associated Alternaria species. Mycologia 96: 119-134. https://doi.org/10.2307/3761993
  24. Pei, D., Xu, X., Feng, Y. and Li, C. 2012. First report of powdery mildew caused by Golovinomyces cichoracearum on Sonchus asper in China. Plant Dis. 96: 766.
  25. Pryor, B. M. and Bigelow, D. M. 2003. Molecular characterization of Embellisia and Nimbya species and their relationship to Alternaria, Ulocladium and Stemphylium. Mycologia 95: 1141-1154. https://doi.org/10.2307/3761916
  26. Simmons, E. G. 1997. Alternaria themes and variations (151-223). Mycotaxon 65: 1-91.
  27. Simmons, E. G. 2007. Alternaria: An Identification Manual. CBS Fungal Biodiversity Centre, Utrecht, Netherlands.
  28. Simmons, E. G. and Roberts, R. G. 1993. Alternaria themes and variations (73). Mycotaxon 48: 109-140.
  29. Sung, G.-H., Sung, J.-M., Hywel-Jones, N. L. and Spatafora, J. W. 2007. A multi-gene phylogeny of Clavicipitaceae (Ascomycota, Fungi): identification of localized incongruence using a combinational bootstrap approach. Mol. Phylogenet. Evol. 44: 1204-1223. https://doi.org/10.1016/j.ympev.2007.03.011
  30. Tanaka, T. 1976. Tanaka's Cyclopedia of Edible Plants of the World. Yugaku-Sha, Tokyo, Japan. 924 pp.
  31. Upadhyay, H., Kumar, A., Gupta, M. K., Sharma, A. and Rahal, A. 2013. Validation of medicinal values of traditionally used Sonchus asper (prickly sow thistle) leaves for the treatment of skin ailments. Adv. Med. Plant Res. 1: 29-35.
  32. White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications, eds. by M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, pp. 315-322. Academic Press, San Diego, CA, USA.
  33. Woudenberg, J. H. C., Groenewald, J. Z., Binder, M. and Crous, P. W. 2013. Alternaria redefined. Stud. Mycol. 75: 171-212. https://doi.org/10.3114/sim0015
  34. Yu, S. H., Cho, H. S., Kim, B.-R. and Park, M. S. 2003. Morphological and molecular characterization of Alternaria isolates from solanaceous crops. Korean J. Mycol. 31: 103-113. https://doi.org/10.4489/KJM.2003.31.2.103
  35. Zhang, J. X., Kang, Y., Xu, T. T., Xu, X. Y., Li, C. X., Chen, K. F. et al. 2021. First report of leaf spot caused by Alternaria alternata on Sonchus asper in China. Plant Dis. 105: 503.