한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제44권3호
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  • Pages.169-174
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  • 2021
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  • 3022-7372(pISSN)
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  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
권호 표지
DOI QR코드

DOI QR Code

봉독의 젖소 유방염 유래 그람 양성 및 음성 세균별 항균효과 분석

Antibacterial effect of bee venom against Gram-positive and negative bacteria isolated from mastitis in dairy cattle

  • 정숙한 (농촌진흥청 국립축산과학원 가축질병방역과) ;
  • 오상익 (농촌진흥청 국립축산과학원 가축질병방역과) ;
  • 이한규 (농촌진흥청 국립축산과학원 가축질병방역과) ;
  • 정영훈 (농촌진흥청 국립축산과학원 가축질병방역과) ;
  • 허태영 (농촌진흥청 국립축산과학원 가축질병방역과) ;
  • 한상미 (농촌진흥청 국립농업과학원 잠사양봉소재과) ;
  • 백귀정 (전라북도 동물위생시험소) ;
  • 조아라 (농촌진흥청 국립축산과학원 가축질병방역과)
  • Jung, Sukhan (Division of Animal Diseases and Health, National Institute of Animal Science, Rural Development Administration) ;
  • Oh, Sang-Ik (Division of Animal Diseases and Health, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Han-Gyu (Division of Animal Diseases and Health, National Institute of Animal Science, Rural Development Administration) ;
  • Jung, Young-Hun (Division of Animal Diseases and Health, National Institute of Animal Science, Rural Development Administration) ;
  • Hur, Tai-Young (Division of Animal Diseases and Health, National Institute of Animal Science, Rural Development Administration) ;
  • Han, Sangmi (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Baek, Kui-Jeong (Jeollabuk-do Institute of Livestock & Veterinary Research) ;
  • Cho, Ara (Division of Animal Diseases and Health, National Institute of Animal Science, Rural Development Administration)
  • 투고 : 2021.09.19
  • 심사 : 2021.09.29
  • 발행 : 2021.09.30
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초록

Mastitis is an inflammatory condition of the mammary gland, most often caused by bacterial infections, resulting in significant economic losses to the dairy industry. Antimicrobial resistance has been of great concern because of the extensive clinical use of antibiotics. For this reason, the development of new compounds as an alternative treatment to bovine mastitis is needed. Bee venom has been widely used as an oriental treatment for several inflammatory diseases and bacterial infections. The aim of the present study was to evaluate the antimicrobial activity of bee venom on bacteria isolated from bovine mastitis. A total of 107 isolates from bovine mastitic milk samples collected in 2019 and 2020 in Jeonbuk province. All bacterial isolates were tested for susceptibility to bee venom of the honey bee (Apis mellifera). In order to obtain comprehensive antibacterial activities of the bee venom, we measured the minimal inhibitory concentration (MIC) of the bee venom against bacterial strains. Bee venom showed significant inhibition of bacterial growth of Gram-negative bacteria Citrobacter spp., Escherchia coli, Klebsiella spp., Pseudomonas spp., Serratia spp. and Raoultella with MIC values of 96, 81, 72, 230, and 85 ㎍/mL, respectively, and Gram-positive bacterial Enterococcus spp., Staphylococcus spp. and Streptococcus spp. with MIC values of 29, 21 and 16 ㎍/mL, respectively. The results indicated that the MIC values were different depending on the bacterial strains, and those of Gram-positive bacteria were lower than those of Gram-negative bacteria for bee venom. These findings suggested that bee venom could be an effective antimicrobial treatment for bovine mastitis; however, further research is necessary to evaluate the mechanism underlying the antimicrobial action, its effectiveness/safety in vivo and effective application for therapeutic use.

키워드

과제정보

본 논문은 농촌진흥청 연구사업(세부과제명: 가축 유래 세포주에서 봉독의 안전성 및 작용 효과 분석, 세부과제번호: PJ014298022021)의 지원 및 2021년도 농촌진흥청(국립축산과학원) 전문연구원 과정 지원사업에 의해 이루어진 것임.

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