Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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Impact of pH on the response of bovine serum albumin to gold surface plasmon resonance chip

소 혈청 알부민의 금 표면 플라즈몬 공명 칩과의 반응에 대한 pH의 영향

  • Sohn, Young-Soo (Department of Biomedical Engineering, Daegu Catholic University)
  • 손영수 (대구가톨릭대학교 의공학과)
  • Received : 2021.09.03
  • Accepted : 2021.09.17
  • Published : 2021.09.30
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Abstract

Reactions between gold (Au) surface plasmon resonance (SPR) chips and bovine serum albumin (BSA) dissolved in solutions of different pH were investigated. The charge on the BSA depends on the pH of the solution in which it is dissolved. Thus, dissolving BSA in different pH solutions resulted in different charges of BSA. Among the BSA dissolved in solutions with pH 4.01, 7.4, and 10.01, the SPR response was the highest for BSA dissolved in the solution of pH 4.01. To eliminate the response variation owing to the difference in the refractive indices of the solutions, phosphate buffered saline (PBS) was injected into the system after the reaction of BSA with the Au SPR chip had happened. In this case too, the BSA dissolved in the solution with pH 4.01 exhibited the highest response. This may be attributed to the non-uniform distribution of ionic patches on the BSA, which can induce electrostatic attraction to the surface even though BSA has a positive charge at pH 4.01, and the absolute values of the net charge of BSA at pH 4.01 and 7.4 were very close.

Keywords

Acknowledgement

본 연구는 2019년 대구가톨릭대학교 연구년 중 수행하였다.

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