Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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Fabrication of a paper-based ELISA to detect polygalacturonase

Polygalacturonase를 검출하기 위한 종이 기반의 효소결합 면역반응 센서 제작

  • Hwang, Young-Kug (Department of Automatic System, Chosun College of Science & Technology) ;
  • Kim, Ji-Kwan (Department of Mechanical Convergence Engineering, Gwangju Unversity) ;
  • Lee, Young Hwan (Department of Automobile, Chunnam Techno University) ;
  • Choi, Young-Soo (Department of Mechanical Convergence Engineering, Gwangju Unversity)
  • 황영국 (조선이공대학교 자동화시스템과) ;
  • 김지관 (광주대학교 융합기계공학과) ;
  • 이영환 (전남과학대학교 자동차과) ;
  • 최영수 (광주대학교 융합기계공학과)
  • Received : 2021.09.11
  • Accepted : 2021.09.27
  • Published : 2021.09.30
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Abstract

In this paper, we describe the fabrication of a paper-based enzyme-linked immunosorbent assay (ELISA) to detect polygalacturonase (PG), which is used as a biomarker to determine whether a plant is infected with a disease. The proposed paper-based ELISA can analyze the concentration of PG in a short time using a small sample compared to the traditional ELISA, which is generally performed using a well plate. To increase the resolution of the sensor, we optimized the dilution ratio of the HRP-conjugated goat anti-rabbit IgG antibody and the dilution ratio of the anti-PG and HRP-conjugated goat anti-rabbit IgG antibodies. Furthermore, for quantitative analysis of PG concentration, Delta RGB analysis was conducted to detect color changes in the sensing window displayed by the PG samples at various concentrations. Based on the experiment, the fabricated paper-based ELISA could measure at least 0.25 ㎍ of PG and the measurement range was 0.25-2 ㎍. Therefore, the paper-based ELISA for detecting PG is expected to be able to determine the presence or absence of disease in crops at the infection stage in the future.

Keywords

Acknowledgement

이 연구는 2021년도 광주대학교 대학 연구비의 지원을 받아 수행되었다.

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