Molecules and Cells

  • 제44권9호
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  • Pages.670-679
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  • 2021
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
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Synaptotagmin 5 Controls SYP132-VAMP721/722 Interaction for Arabidopsis Immunity to Pseudomonas syringae pv tomato DC3000

  • Kim, Soohong (Department of Molecular Biology, Dankook University) ;
  • Kim, Hyeran (Department of Biological Sciences, Kangwon National University) ;
  • Park, Keunchun (Department of Molecular Biology, Dankook University) ;
  • Cho, Da Jeong (Department of Molecular Biology, Dankook University) ;
  • Kim, Mi Kyung (Department of Molecular Biology, Dankook University) ;
  • Kwon, Chian (Department of Molecular Biology, Dankook University) ;
  • Yun, Hye Sup (Department of Biological Sciences, Konkuk University)
  • 투고 : 2021.04.16
  • 심사 : 2021.08.08
  • 발행 : 2021.09.30
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초록

Vesicle-associated membrane proteins 721 and 722 (VAMP721/722) are secretory vesicle-localized arginine-conserved soluble N-ethylmaleimide-sensitive factor attachment protein receptors (R-SNAREs) to drive exocytosis in plants. They are involved in diverse physiological processes in plants by interacting with distinct plasma membrane (PM) syntaxins. Here, we show that synaptotagmin 5 (SYT5) is involved in plant defense against Pseudomonas syringae pv tomato (Pst) DC3000 by regulating SYP132-VAMP721/722 interactions. Calcium-dependent stimulation of in vitro SYP132-VAMP722 interaction by SYT5 and reduced in vivo SYP132-VAMP721/722 interaction in syt5 plants suggest that SYT5 regulates the interaction between SYP132 and VAMP721/722. We interestingly found that disease resistance to Pst DC3000 bacterium but not to Erysiphe pisi fungus is compromised in syt5 plants. Since SYP132 plays an immune function to bacteria, elevated growth of surface-inoculated Pst DC3000 in VAMP721/722-deficient plants suggests that SYT5 contributes to plant immunity to Pst DC3000 by promoting the SYP132-VAMP721/722 immune secretory pathway.

키워드

과제정보

This work was supported by a 2019 Research Grant (520190026 to H.K.) from Kangwon National University, Korea, a grant (PJ01477001 to C.K.) from Rural Development Administration, Korea, a grant (P0016045 to C.K.) from Korea Institute for Advancement of Technology, Korea, and grants (2016R1D1A1B02007322 to C.K.; 2021R1F1A1063111 to H.S.Y.) from National Research Foundation, Korea. We thank Hae Ri Kwon and Yunjin Choi for technical assistance.

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