DOI QR코드

DOI QR Code

𝛽-Patchoulene: Conversion from Patchouli Alcohol by Acid Catalysts and its In silico Anti-inflammatory Study

  • 투고 : 2021.05.28
  • 심사 : 2021.09.24
  • 발행 : 2021.09.30

초록

𝛽-Patchoulene (𝛽-PAE) is a tricyclic sesquiterpene which performed many potential bioactivities and can be found in patchouli oil but in very low concentration. This study aimed to obtained 𝛽-PAE in high concentration by conversion of patchouli alcohol (PA) in patchouli oil under acid catalyzed reaction. Patchouli oil was fractinated by vacuum distillation at 96 kPa to get the fraction with the highest PA content. H2SO4 and ZnCl2 were used respectively as homogeneous and heterogeneous acid catalysts in the conversion reaction of the selected fraction. Patchouli oil, the fractions and the products were analysed by using GC-MS and FTIR instruments. Moreover, the interaction of 𝛽-PAE to COX-2 protein was studied to understand the antiinflammation activity of 𝛽-PAE. The results showed that patchouli oil contains 25.3% of PA. The selected fraction which has the highest PA content (70.3%) was distilled at 151 - 152 ℃. The application of ZnCl2 catalyst in conversion reaction did not succeed. In contrast, H2SO4 as a catalyst in acetic acid solvent succeeded in converting the overall fraction of PA to 𝛽-PAE. Furthermore, the molecular docking study of 𝛽-PAE against COX-2 enzyme showed van der Waals and alkyl-alkyl stacking interactions on ten amino acid residues.

키워드

과제정보

The authors thank the chemical laboratory staff of the Department of Chemical Engineering, Polytechnic State of Ujung Pandang, for their assistance in performing the FTIR and GC-MS analysis of our samples.

참고문헌

  1. Arpi, N.; Erika, C.; Ermaya, D. Proceedings of The Annual International Conference Syiah Kuala University 2011, 2011, 22-27.
  2. Muyassaroh.; Daryono, E. D.; Hudha, M. I. Int. J. ChemTech Res. 2016, 9, 108-116.
  3. Harunsyah.; Yunus, M. Proc. 2nd Annu. Int. Conf. Syiah Kuala Univ. 2012 8th IMT-GT Uninet Biosci. Conf. 2012, 2, 149-153.
  4. Kusuma, H. S.; Mahfud, M. J. Appl. Res. Med. Aromat. Plants 2017, 4, 46-54. https://doi.org/10.1016/j.jarmap.2016.08.001
  5. Zhao, Z.; Lu, J.; Leung, K.; Chan, C. L.; Jiang, Z. H. Chem. Pharm. Bull (Tokyo). 2005, 53, 856-860. https://doi.org/10.1248/cpb.53.856
  6. Chen, W. K.; Tseng, H. H.; Wei, M. C.; Su, E. C.; Chiu, I. C. Int. J. Hydrogen Energy 2014, 39, 19555-19562. https://doi.org/10.1016/j.ijhydene.2014.08.154
  7. Lal, M.; Pandey, S. K.; Dutta, S.; Munda, S.; Baruah, J.; Paw, M. J. Essent. Oil Bearing Plants 2018, 21, 131-138. https://doi.org/10.1080/0972060X.2018.1449670
  8. Cornwell, C. P. J. Essent. Oil Res. 2010, 22, 360-364. https://doi.org/10.1080/10412905.2010.9700346
  9. Swamy, M. K.; Mohanty, S. K.; Sinniah, U. R.; Maniyam, A. J. Essent. Oil Bear. Plants 2015, 18, 826-832. https://doi.org/10.1080/0972060X.2015.1029989
  10. Bergonzi, M. C.; Bilia, A. R.; Gallori, S.; Guerrini, D.; Vincieri, F. F. Drug Dev. Ind. Pharm. 2001, 27, 491-497. https://doi.org/10.1081/DDC-100105173
  11. Hariyani; Widaryanto, E.; Herlina, N. J. Produksi Tanam. 2015, 3, 205-211.
  12. Hu, G.; Peng, C.; Xie, X.; Zhang, S.; Cao, X. Evid. Based Complement. Altern. Med. 2017, 2017, 4850612. https://doi.org/10.1155/2017/4850612
  13. Ambrose, D. C. P.; Annamalai, S. J. K.; Naik, R. Indian J. Sci. Technol. 2013, 6, 5559-5562.
  14. Yahya, A.; Yunus, R. M. Procedia Eng. 2013, 53, 1-6. https://doi.org/10.1016/j.proeng.2013.02.001
  15. Kusuma, H. S.; Mahfud, M. Int. Food Res. J. 2017, 24, 1525-1528.
  16. Miyazawa, M.; Shimabayashi, H.; Hayashi, S.; Hashimoto, S.; Nakamura, S. I.; Kosaka, H.; Kameoka, H. J. Agric. Food Chem. 2000, 48, 5406-5410. https://doi.org/10.1021/jf000346t
  17. Singh, M.; Sharma, S.; Ramesh, S. Ind. Crops Prod. 2002, 16, 101-107. https://doi.org/10.1016/S0926-6690(02)00013-4
  18. Xu, X.; Tang, Z.; Liang, Y. Anal. Methods 2010, 2, 359-367. https://doi.org/10.1039/b9ay00213h
  19. Ravindra, N. S.; Ramesh, S. I.; Gupta, M. K.; Jhang, T.; Shukla, A. K.; Darokar, M. P.; Kulkarni, R. N. J. Crop Sci. Biotechnol. 2012, 15, 33-39. https://doi.org/10.1007/s12892-011-0068-5
  20. Chen, Y.; Wu, Y. G.; Xu, Y.; Zhang, J. F.; Song, X. Q.; Zhu, G. P.; Hu, X. W. Rev. Bras. Farmacogn. 2014, 24, 626-634. https://doi.org/10.1016/j.bjp.2014.11.001
  21. Xian, Y. F.; Li, Y. C.; Ip, S. P.; Lin, Z. X.; Lai, X. P.; Su, Z. R. Exp. Ther. Med. 2011, 2, 545-550. https://doi.org/10.3892/etm.2011.233
  22. Chakrapani, P.; Venkatesh, K.; Chandra Sekhar Singh, B.; Arun Jyothi, B.; Kumar, P.; Amareshwari, P.; Roja Rani, A. Int. J. Pharm. Sci. Rev. Res. 2013, 21, 7-15.
  23. Karimi, A. Adv. Environ. Biol. 2014, 8, 2301-2309.
  24. Munda, S.; Dutta, S.; Pandey, S. K.; Sarma, N.; Lal, M. J. Essent. Oil Bearing Plants 2019, 22, 105-119. https://doi.org/10.1080/0972060X.2019.1601032
  25. Swamy, M. K.; Sinniah, U. R. Molecule 2015, 20, 8521-8547. https://doi.org/10.3390/molecules20058521
  26. Zhang, Z.; Chen, X.; Chen, H.; Wang, L.; Liang, J.; Luo, D.; Liu, Y.; Yang, H.; Li, Y.; Xie, J.; Su, Z. Eur. J. Pharmacol. 2016, 781, 229-238. https://doi.org/10.1016/j.ejphar.2016.04.028
  27. Liang, J. L.; Wu, J. Z.; Liu, Y. H.; Zhang, Z. B.; Wu, Q. D.; Chen, H. B.; Huang, Y. F.; Dou, Y. X.; Zhou, J. T.; Su, Z. R.; Zhan, J. Y. X. Mediators Inflamm. 2017, 2017, 1089028.
  28. Yang, W. H.; Liu, Y. H.; Liang, J. L.; Lin, Z. X.; Kong, Q. L.; Xian, Y. F.; Guo, D. Q.; Lai, Z. Q.; Su, Z. R.; Huang, X. Q. Eur. J. Inflamm. 2017, 15, 136-141. https://doi.org/10.1177/1721727X17714694
  29. Dechayont, B.; Ruamdee, P.; Poonnaimuang, S.; Mokmued, K.; Chunthorng-Orn, J. J. Bot. 2017, 2017, 8310275.
  30. Liu, Y.; Liang, J.; Wu, J.; Chen, H.; Zhang, Z.; Yang, H.; Chen, L.; Chen, H.; Su, Z.; Li, Y. Sci. Rep. 2017, 7, 5591. https://doi.org/10.1038/s41598-017-05996-5
  31. Leong, W.; Huang, G.; Khan, I.; Xia, W.; Li, Y.; Liu, Y.; Li, X.; Han, R.; Su, Z.; Hsiao, W. L. W. Front. Pharmacol. 2019, 10, 1229. https://doi.org/10.3389/fphar.2019.01229
  32. Singh, B. R.; Sinha, D. K.; Vinodh Kumar, O. R.; Abhijit, M. P.; Ujjwal Kumar, D. E.; Gupta, V. K. World J. Pharm. Sci. 2019, 7, 47-65.
  33. Zhang, F. B.; Wang, J. P.; Zhang, H. X.; Fan, G. M.; Cui, X. Exp. Ther. Med. 2019, 17, 3335-3342.
  34. Chen, X. Y.; Dou, Y. X.; Luo, D. D.; Zhang, Z. B.; Li, C. L.; Zeng, H. F.; Su, Z. F.; Xie, J. H.; Lai, X. P.; Li, Y. C. Int. Immunopharmacol. 2017, 50, 270-278. https://doi.org/10.1016/j.intimp.2017.07.001
  35. Wu, J. Z.; Liu, Y. H.; Liang, J. L.; Huang, Q. H.; Dou, Y. X.; Nie, J.; Zhuo, J. Y.; Wu, X.; Chen, J. N.; Su, Z. R.; Wu, Q. D. Phytomedicine 2018, 39, 111-118. https://doi.org/10.1016/j.phymed.2017.12.024
  36. Su, Z. Q.; Wu, X. L.; Bao, M. J.; Li, C. W.; Kong, S. Z.; Su, Z. R.; Lai, X. P.; Li, Y. C.; Chen, J. N. Trop. J. Pharm. Res. 2014, 13, 359-363. https://doi.org/10.4314/tjpr.v13i3.7
  37. Morris, G. M.; Huey, R.; Lindstrom, W.; Sanner, M. F.; Belew, R. K.; Goodsell, D. S.; Olson, A. J. J. Comput. Chem. 2009, 30, 2785-2791. https://doi.org/10.1002/jcc.21256
  38. Pettersen, E. F.; Goddard, T. D.; Huang, C. C.; Couch, G. S.; Greenblatt, D. M.; Meng, E. C.; Ferrin, T. E. J. Comput. Chem. 2004, 25, 1605-1612. https://doi.org/10.1002/jcc.20084
  39. Dassault Systemes. Biovia Discovery Studio Visualizer; Dassault Systemes: San Diego, 2019.
  40. Hanwell, M. D.; Curtis, D. E.; Lonie, D. C.; Vandermeersch, T.; Zurek, E.; Hutchison, G. R. J. Cheminform. 2012, 4, 17. https://doi.org/10.1186/1758-2946-4-17
  41. Morris, G. M.; Goodsell, D. S.; Halliday, R. S.; Huey, R.; Hart, W. E.; Belew, R. K.; Olson, A. J. J. Comput. Chem. 1998, 19, 1639-1662. https://doi.org/10.1002/(SICI)1096-987X(19981115)19:14<1639::AID-JCC10>3.0.CO;2-B
  42. Hardjo, P. H.; Susanto, D. P. S.; Savitri, W. D.; Purwanto, M. G. M. Nusant. Biosci. 2019, 11, 123-127. https://doi.org/10.13057/nusbiosci/n110202
  43. Kusuma, H. S.; Mahfud, M. Period. Polytech. Chem. Eng. 2017, 61, 82-92.
  44. Sundaresan, V.; Singh, S. P.; Mishra, A. N.; Shasany, A. K.; Darokar, M. P.; Kalra, A.; Naqvi, A. A. J. Essent. Oil Res. 2009, 21, 220-222. https://doi.org/10.1080/10412905.2009.9700152
  45. Ma'mun; Maryadhi, A. Bul. Littro 2008, XIX, 95-99.
  46. Asnawi, T. M.; Alam, P. N.; Husin, H.; Zaki, M. IOP Conf. Ser. Mater. Sci. Eng. 2018, 345.
  47. Pu, Q.; Liang, J.; Shen, Q.; Fu, J.; Pu, Z.; Liu, J.; Wang, X.; Wang, Q. Genes (Basel) 2019, 10, 441. https://doi.org/10.3390/genes10060441