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Effect of epidural polydeoxyribonucleotide in a rat model of lumbar foraminal stenosis

  • Lee, Ho-Jin (Department of Anesthesiology and Pain Medicine, Seoul National University Hospital) ;
  • Ju, Jiyoun (Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital) ;
  • Choi, Eunjoo (Department of Anesthesiology and Pain Medicine, Seoul National University College of Medicine) ;
  • Nahm, Francis Sahngun (Department of Anesthesiology and Pain Medicine, Seoul National University College of Medicine) ;
  • Choe, Ghee Young (Department of Pathology, Seoul National University Bundang Hospital) ;
  • Lee, Pyung Bok (Department of Anesthesiology and Pain Medicine, Seoul National University College of Medicine)
  • Received : 2021.04.11
  • Accepted : 2021.06.17
  • Published : 2021.10.01

Abstract

Background: We aimed to investigate the effect of epidural polydeoxyribonucleotide (PDRN) on mechanical allodynia and motor dysfunction in a rat model of lumbar foraminal stenosis (LFS). Methods: This study was conducted in two stages, using male Sprague-Dawley rats. The rats were randomly divided into eight groups. In the first stage, the groups were as follows: vehicle (V), sham (S), and epidural PDRN at 5 (P5), 8 (P8), and 10 (P10) mg/kg; and in the second stage, they were as follows: intraperitoneal PDRN 8 mg/kg, epidural 3,7-dimethyl-1-propargilxanthine (DMPX) (0.1 mg/kg), and DMPX (0.1 mg/kg). The LFS model was established, except for the S group. After an epidural injection of the test solutions, von Frey and treadmill tests were conducted for 3 weeks. Subsequently, histopathologic examinations were conducted in the V, S, P5, and P10 groups. Results: A total of 65 rats were included. The P8 and P10 groups showed significant recovery from mechanical allodynia and motor dysfunction at all time points after drug administration compared to the V group. These effects were abolished by concomitant administration of DMPX. On histopathological examination, no epineurial inflammation or fibrosis was observed in the epidural PDRN groups. Conclusions: Epidural injection of PDRN significantly improves mechanical allodynia and motor dysfunction in a rat model of LFS, which is mediated by the spinal adenosine A2A receptor. The present data support the need for further research to determine the role of epidural PDRN in spinal stenosis treatment.

Keywords

Acknowledgement

The authors appreciate the Division of Statistics in the Medical Research Collaborating Center at Seoul National University Bundang Hospital, Korea, for the assistance with the statistical analyses. The authors thank Mr. Myung Hun Park for his assistance with animal care and establishing an experimental model.

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