International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Inhibition of biofilm formation of periodontal pathogens by D-Arabinose

  • An, Sun-Jin (Department of Oral Microbiology and Immunology, School of Dentistry, Seoul National University) ;
  • Namkung, Jong-Uk (Department of Oral Microbiology and Immunology, School of Dentistry, Seoul National University) ;
  • Ha, Kyung-Won (Bone Science R&D Center, OSSTEM IMPLANT Co., Ltd) ;
  • Jun, Hye-Kyoung (Bone Science R&D Center, OSSTEM IMPLANT Co., Ltd) ;
  • Kim, Hyun Young (Department of Oral Microbiology and Immunology, School of Dentistry, Seoul National University) ;
  • Choi, Bong-Kyu (Department of Oral Microbiology and Immunology, School of Dentistry, Seoul National University)
  • Received : 2021.08.26
  • Accepted : 2021.09.01
  • Published : 2021.09.30
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Abstract

Periodontitis and periimplantitis are caused as a result of dental biofilm formation. This biofilm is composed of multiple species of pathogens. Therefore, controlling biofilm formation is critical for disease prevention. To inhibit biofilm formation, sugars can be used to interrupt lectin-involving interactions between bacteria or between bacteria and a host. In this study, we evaluated the effect of D-Arabinose on biofilm formation of putative periodontal pathogens as well as the quorum sensing activity and whole protein profiles of the pathogens. Crystal violet staining, confocal laser scanning microscopy, and scanning electron microscopy revealed that D-Arabinose inhibited biofilm formation of Porphyromonas gingivalis, Fusobacterium nucleatum, and Tannerella forsythia. D-Arabinose also significantly inhibited the activity of autoinducer 2 of F. nucleatum and the expression of representative bacterial virulence genes. Furthermore, D-Arabinose treatment altered the expression of some bacterial proteins. These results demonstrate that D-Arabinose can be used as an antibiofilm agent for the prevention of periodontal infections.

Keywords

Acknowledgement

This work was supported by the World Class 300 Project (R&D) from Korea Institute for Advancement of Technology (grant number P108100012) and the Dental Research Institute of Seoul National University.

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