International Journal of Oral Biology

  • 제46권3호
  • /
  • Pages.127-133
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  • 2021
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  • 1226-7155(pISSN)
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  • 2287-6618(eISSN)
대한구강생물학회 (The Korean Academy of Oral Biology)
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Role of γ-glutamyltranspeptidase in osteoclastogenesis induced by Fusobacterium nucleatum

  • Kim, Aeryun (Department of Applied Life Science, The Graduate School, Yonsei University) ;
  • Kim, Ji-Hye (Department of Dental Hygiene, Baekseok University)
  • 투고 : 2021.07.27
  • 심사 : 2021.09.03
  • 발행 : 2021.09.30
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초록

We previously showed that γ-glutamyltranspeptidase (GGT), an enzyme involved in glutathione metabolism, in Bacillus subtilis acts as a virulence factor for osteoclastogenesis via the RANKL-dependent pathway. Hence, it can be hypothesized that GGT of periodontopathic bacteria acts as a virulence factor in bone destruction. Because Fusobacterium nucleatum, which is a periodontopathic pathogen, has GGT with a primary structure similar to that of B. subtilis GGT (37.7% identify), the bone-resorbing activity of F. nucleatum GGT was examined here. Recombinant GGT (rGGT) of F. nucleatum was expressed in Escherichia coli and purified using the His tag of rGGT. F. nucleatum rGGT (Fn rGGT) was expressed as a precursor of GGT, and then processed to a heavy subunit and a light subunit, which is characteristic of general GGTs, including the human and B. subtilis enzymes. Osteoclastogenesis was achieved in a co-culture system of mouse calvaria-derived osteoblasts and bone marrow cells. Fn rGGT induced osteoclastogenesis to a level similar to that of B. subtilis rGGT; furthermore, osteoclastogenesis was induced in a dose-dependent manner. These results suggest that F. nucleatum GGT possesses a virulent bone-resorbing activity, which could play an important role in the pathogenesis of periodontitis.

키워드

과제정보

We would like to thank Joong-Ki Kook (School of Dentistry, Chosun University) for providing Fusobacterium nucleatum strain used in this work.

참고문헌

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