Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Anti-adipocyte differentiation activity and flavonoid content determination by HPLC/UV analysis of tree sprouts

  • Kim, Juree (Department of Plant Science and Technology, Chung-Ang University) ;
  • Jang, Taewon (Department of Biomedical Science, Jungwon University) ;
  • Kim, Ji Hyun (Department of Food Science, Gyeongsang National University) ;
  • Shin, Hanna (Department of Forest Bioresources, National Institute of Forest Science) ;
  • Park, Jaeho (Department of Biomedical Science, Jungwon University) ;
  • Lee, Sanghyun (Department of Plant Science and Technology, Chung-Ang University)
  • Received : 2021.07.13
  • Accepted : 2021.08.02
  • Published : 2021.09.30
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Abstract

The in vitro anti-obesity activity of 12 species of tree sprouts in differentiated 3T3-L1 cells and the mechanisms underlying their activity were evaluated. (+)-Catechin and quercetin concentrations in the sprouts were analyzed by HPLC/UV at 270 and 254 nm, respectively. Euonymus alatus (EAT) and Fraxinus mandschuria (FMS) extracts at doses of 50 and 100 ㎍/mL inhibited the accumulation of lipid droplets in differentiated 3T3-L1 cells. Moreover, EAT and FMS downregulated the expression of the CCAAT/enhancer-binding protein-α, adipogenesis-related proteins peroxisome proliferator-activated receptor-γ, and adipocyte P-2α in differentiated 3T3-L1 cells. Tree sprouts with an abundant flavonoid content exerted the highest anti-obesity activity. Concentrations of total flavonoids were the highest in FMS (24.281 mg/g DW) sprouts. These findings could be used to develop health-promoting functional foods or supplements derived from tree sprouts.

Keywords

Acknowledgement

This work was supported by the Research Program for Forest Science & Technology Development of the National Institute of Forest Science (Project No. FG0403-2018-03).

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