Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Helicobacter pylori GroEL Seropositivity Is Associated with an Increased Risk of Opisthorchis viverrini-Associated Hepatobiliary Abnormalities and Cholangiocarcinoma

  • Jala, Isabelle (Tropical Medicine Graduate Program (International Program), Academic Affairs, Faculty of Medicine, Khon Kaen University) ;
  • Almanfaluthi, Muhammad Luthfi (Tropical Medicine Graduate Program (International Program), Academic Affairs, Faculty of Medicine, Khon Kaen University) ;
  • Laha, Thewarach (Department of Parasitology, Faculty of Medicine, Khon Kaen University) ;
  • Kanthawong, Sakawrat (Department of Microbiology, Faculty of Medicine, Khon Kaen University) ;
  • Tangkawattana, Sirikachorn (WHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver Fluke Disease), Tropical Disease Research Centre, Faculty of Medicine, Khon Kaen University) ;
  • Saichua, Prasert (Tropical Medicine Graduate Program (International Program), Academic Affairs, Faculty of Medicine, Khon Kaen University) ;
  • Suttiprapa, Sutas (Tropical Medicine Graduate Program (International Program), Academic Affairs, Faculty of Medicine, Khon Kaen University) ;
  • Sripa, Banchob (WHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver Fluke Disease), Tropical Disease Research Centre, Faculty of Medicine, Khon Kaen University)
  • Received : 2021.04.22
  • Accepted : 2021.08.02
  • Published : 2021.08.31
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Abstract

Despite the synergistic effect of Opisthorchis viverrini and Helicobacter pylori co-infection on pathogenesis of severe hepatobiliary abnormalities (HBA) including advanced periductal fibrosis and replace with cholangiocarcinoma (CCA) have been established, the immune response to H. pylori in O. viverrini infected population has never been explored. Hence, this study aimed to investigate the antibody responses to 2 immunogenic H. pylori proteins in O. viverrini-infected patients with HBA and CCA. The risk analysis by multinomial logistic regression revealed that GroEL seropositivity was associated with higher risks of hepatobiliary abnormalities and CCA with adjusted odds ratios (95% confidence intervals) of 2.11 (95% CI=1.20-3.71, P=0.008) and 2.13 (95% CI=1.21-3.75, P=0.009), respectively. These findings indicate that GroEL seropositivity might be a biomarker for early detection of O. viverrini associated HBA and CCA.

Keywords

Acknowledgement

This work was supported by the Thailand Research Fund Senior Scholar (RTA 5680006). BS is a TRF Senior Research Scholar. IJ received a scholarship from the Faculty of Medicine, Khon Kaen University. We thank Professor Yukifuma Nawa for numerous discussions and for his assistance in obtaining the commercial antibody determination kit E-Plate Eiken H. pylori antibody (Eiken Chemical Co., Ltd., Tokyo, Japan). We acknowledge the advice of Professors Ratha-Korn Vilaichone (Thammasat University) and Veerasak Punyapornwithaya (Chiang Mai University). We also thank Manop Sripa (Khon Kaen University) for his technical assistance.

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