Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

LINC00174 Facilitates Proliferation and Migration of Colorectal Cancer Cells via MiR-3127-5p/ E2F7 Axis

  • Ma, Yuhong (Department of Gastroenterology, People's Hospital of Ningxia Hui Autonomous Region) ;
  • Li, Yuzhen (Department of Gastroenterology, People's Hospital of Ningxia Hui Autonomous Region) ;
  • Tang, Yuanyuan (Department of Gastroenterology, People's Hospital of Ningxia Hui Autonomous Region) ;
  • Tang, Ning (Department of Gastroenterology, People's Hospital of Ningxia Hui Autonomous Region) ;
  • Wang, Dengke (Department of Anatomy, Ningxia Medical University) ;
  • Li, Xiaofei (Department of Gastroenterology, People's Hospital of Ningxia Hui Autonomous Region)
  • Received : 2021.03.01
  • Accepted : 2021.06.26
  • Published : 2021.08.28
Download PDF
⟨ Previous Next ⟩

Abstract

The literature indicates that LINC00174 promotes the growth of colorectal cancer (CRC) cells, but its research needs to be enriched. We tried to explore the function and mechanism of LINC00174 in CRC cell proliferation and migration. Bioinformatics analysis predicted the binding relationship and expressions of lncRNA, miRNA and mRNA. Clinical study analyzes the relationship between LINC00174 and clinical data characteristics of CRC patients. The expressions of LINC00174, miR-3127-5p and E2F7 were verified by RT-qPCR, and the combination of the two was verified by dual luciferase analysis and RNA immunoprecipitation as needed. Western blot was used to detect the expression of EMT-related protein and E2F7 protein. Functional experiments were used to evaluate the function of the target gene on CRC cells. LINC00174 was up-regulated in CRC clinical samples and cells and was related to the clinical characteristics of CRC patients. High-expression of LINC00174, contrary to the effect of siLINC00174, promoted cell viability, proliferation, migration and invasion, up-regulated the expressions of N-Cadherin, Vimentin, E2F7, and inhibited the expression of E-Cadherin. MiR-3127-5p was one of the targeted miRNAs of LINC00174 and was down-regulated in CRC samples. In addition, miR-3127-5p mimic partially reversed the malignant phenotype of CRC cells induced by LINC00174. Besides, E2F7 was a target gene of miR-3127-5p, and LINC00174 repressed miR-3127-5p to regulate E2F7. Our research reveals that LINC00174 affected the biological characteristics of CRC cells through regulated miR-3127-5p/ E2F7 axis.

Keywords

Acknowledgement

This work was supported by Ningxia Hui Autonomous Region Key R&D Program General Project[2019BEG03055].

References

  1. Patel SG, Ahnen DJ. 2018. Colorectal cancer in the young. Curr. Gastroenterol. Rep. 20: 15. https://doi.org/10.1007/s11894-018-0618-9
  2. Cao H, Xu E, Liu H, Wan L, Lai M. 2015. Epithelial-mesenchymal transition in colorectal cancer metastasis: a system review. Pathol. Res. Pract. 211: 557-569. https://doi.org/10.1016/j.prp.2015.05.010
  3. Modest DP, Pant S. 2019. Sartore-Bianchi A. Treatment sequencing in metastatic colorectal cancer. Eur. J. Cancer 109: 70-83. https://doi.org/10.1016/j.ejca.2018.12.019
  4. Marmol I, Sanchez-de-Diego C, Pradilla Dieste A, Cerrada E, Rodriguez Yoldi MJ. 2017. Colorectal carcinoma: a general overview and future perspectives in colorectal cancer. Int. J. Mol. Sci. 18: 197. https://doi.org/10.3390/ijms18010197
  5. Yiu AJ, Yiu CY. 2016. Biomarkers in colorectal cancer. Anticancer Res. 36: 1093-1102.
  6. Li H, Ma SQ, Huang J, Chen XP, Zhou HH. 2017. Roles of long noncoding RNAs in colorectal cancer metastasis. Oncotarget 8: 39859-39876. https://doi.org/10.18632/oncotarget.16339
  7. Yin DD, Liu ZJ, Zhang E, Kong R, Zhang ZH, Guo RH. 2015. Decreased expression of long noncoding RNA MEG3 affects cell proliferation and predicts a poor prognosis in patients with colorectal cancer. Tumour Biol. 36: 4851-4859. https://doi.org/10.1007/s13277-015-3139-2
  8. Liang WC, Fu WM, Wong CW, Wang Y, Wang WM, Hu GX, et al. 2015. The lncRNA H19 promotes epithelial to mesenchymal transition by functioning as miRNA sponges in colorectal cancer. Oncotarget 6: 22513-22525. https://doi.org/10.18632/oncotarget.4154
  9. Ding J, Lu B, Wang J, Wang J, Shi Y, Lian Y, et al. 2015. Long non-coding RNA Loc554202 induces apoptosis in colorectal cancer cells via the caspase cleavage cascades. J. Exp. Clin. Cancer Res. 34: 100. https://doi.org/10.1186/s13046-015-0217-7
  10. Shi J, Zhang Y, Qin B, Wang Y, Zhu X. 2019. Long non-coding RNA LINC00174 promotes glycolysis and tumor progression by regulating miR-152-3p/SLC2A1 axis in glioma. J. Exp.Clin. Cancer Res. 38: 395. https://doi.org/10.1186/s13046-019-1390-x
  11. Li B, Zhao H, Song J, Wang F, Chen M. 2020. LINC00174 down-regulation decreases chemoresistance to temozolomide in human glioma cells by regulating miR-138-5p/SOX9 axis. Human Cell. 33: 159-174. https://doi.org/10.1007/s13577-019-00281-1
  12. Wang Z, Wang Q, Bao Z, Guo L, Chen H, Lv T, et al. 2020. LINC00174 is a favorable prognostic biomarker in glioblastoma via promoting proliferative phenotype. Cancer Biomark. 28: 421-427. https://doi.org/10.3233/CBM-191026
  13. Zhao JT, Chi BJ, Sun Y, Chi NN, Zhang XM, Sun JB, et al. 2020. LINC00174 is an oncogenic lncRNA of hepatocellular carcinoma and regulates miR-320/S100A10 axis. Cell Biochem. Funct. 38: 859-869. https://doi.org/10.1002/cbf.3498
  14. Tito C, Ganci F, Sacconi A, Masciarelli S, Fontemaggi G, Pulito C, et al. 2020. LINC00174 is a novel prognostic factor in thymic epithelial tumors involved in cell migration and lipid metabolism. Cell Death Dis. 11: 959. https://doi.org/10.1038/s41419-020-03171-9
  15. Liu J, Yang T, Zhang Y, Wang S. 2020. Promotion of BZW2 by LINC00174 through miR-4500 inhibition enhances proliferation and apoptosis evasion in laryngeal papilloma. Cancer Cell Int. 20: 471. https://doi.org/10.1186/s12935-020-01559-3
  16. Shen Y, Gao X, Tan W, Xu T. 2018. STAT1-mediated upregulation of lncRNA LINC00174 functions a ceRNA for miR-1910-3p to facilitate colorectal carcinoma progression through regulation of TAZ. Gene 666: 64-71. https://doi.org/10.1016/j.gene.2018.05.001
  17. Tang X, Qiao X, Chen C, Liu Y, Zhu J, Liu J. 2019. Regulation mechanism of long noncoding RNAs in colon cancer development and progression. Yonsei Med. J. 60: 319-325. https://doi.org/10.3349/ymj.2019.60.4.319
  18. Xie X, Tang B, Xiao YF, Xie R, Li BS, Dong H, et al. 2016. Long non-coding RNAs in colorectal cancer. Oncotarget 7: 5226-5239. https://doi.org/10.18632/oncotarget.6446
  19. Chen DL, Lu YX, Zhang JX, Wei XL, Wang F, Zeng ZL, et al. 2017. Long non-coding RNA UICLM promotes colorectal cancer liver metastasis by acting as a ceRNA for microRNA-215 to regulate ZEB2 expression. Theranostics 7: 4836-4849. https://doi.org/10.7150/thno.20942
  20. Miao C, Lei M, Hu W, Han S, Wang Q. 2017. A brief review: the therapeutic potential of bone marrow mesenchymal stem cells in myocardial infarction. Stem Cell Res. Ther. 8: 242. https://doi.org/10.1186/s13287-017-0697-9
  21. Ding D, Li C, Zhao T, Li D, Yang L, Zhang B. 2018. LncRNA H19/miR-29b-3p/PGRN axis promoted epithelial-mesenchymal transition of colorectal cancer cells by acting on Wnt signaling. Mol. Cells 41: 423-435. https://doi.org/10.14348/MOLCELLS.2018.2258
  22. Yang Y, Sun Y, Wu Y, Tang D, Ding X, Xu W, et al. 2018. Downregulation of miR-3127-5p promotes epithelial-mesenchymal transition via FZD4 regulation of Wnt/beta-catenin signaling in non-small-cell lung cancer. Mol. Carcinog. 57: 842-853. https://doi.org/10.1002/mc.22805
  23. Pan B, Zhao M, Xu L. 2019. Long noncoding RNA gastric cancer-associated transcript 3 plays oncogenic roles in glioma through sponging miR-3127-5p. J. Cell Physiol. 234: 8825-8833. https://doi.org/10.1002/jcp.27542
  24. Ding B, Yao M, Fan W, Lou W. 2020. Whole-transcriptome analysis reveals a potential hsa_circ_0001955/hsa_circ_0000977-mediated miRNA-mRNA regulatory sub-network in colorectal cancer. Aging (Albany NY). 12: 5259-5279. https://doi.org/10.18632/aging.102945
  25. Wang W, Xie Y, Chen F, Liu X, Zhong LL, Wang HQ, et al. 2019. LncRNA MEG3 acts a biomarker and regulates cell functions by targeting ADAR1 in colorectal cancer. World J. Gastroenterol. 25: 3972-3984. https://doi.org/10.3748/wjg.v25.i29.3972
  26. Yang MH, Hu ZY, Xu C, Xie LY, Wang XY, Chen SY, et al. 2015. MALAT1 promotes colorectal cancer cell proliferation/migration/invasion via PRKA kinase anchor protein 9. Biochim. Biophys. Acta 1852: 166-174. https://doi.org/10.1016/j.bbadis.2014.11.013
  27. Xu Y, Zhang X, Hu X, Zhou W, Zhang P, Zhang J, et al. 2018. The effects of lncRNA MALAT1 on proliferation, invasion and migration in colorectal cancer through regulating SOX9. Mol. Med. 24: 52. https://doi.org/10.1186/s10020-018-0050-5
  28. Guo J, Shen S, Liu X, Ruan X, Zheng J, Liu Y, et al. 2019. Role of linc00174/miR-138-5p (miR-150-5p)/FOSL2 feedback loop on regulating the blood-tumor barrier permeability. Mol. Ther. Nucleic Acids 18: 1072-1090. https://doi.org/10.1016/j.omtn.2019.10.031
  29. Wang Z, Wang Q, Bao Z, Guo L, Chen H, Lv T, et al. 2020. LINC00174 is a favorable prognostic biomarker in glioblastoma via promoting proliferative phenotype. Cancer Biomark. 28: 421-427. https://doi.org/10.3233/CBM-191026
  30. Zhao JT, Chi BJ, Sun Y, Chi NN, Zhang XM, Sun JB, et al. 2020. LINC00174 is an oncogenic lncRNA of hepatocellular carcinoma and regulates miR-320/S100A10 axis. Cell Biochem. Funct. 38: 859-869. https://doi.org/10.1002/cbf.3498
  31. Tang XJ, Wang W, Hann SS. 2019. Interactions among lncRNAs, miRNAs and mRNA in colorectal cancer. Biochimie 163: 58-72. https://doi.org/10.1016/j.biochi.2019.05.010
  32. Liu W, Song Y, Zhang C, Gao P, Huang B, Yang J. 2018. The protective role of all-transretinoic acid (ATRA) against colorectal cancer development is achieved via increasing miR-3666 expression and decreasing E2F7 expression. Biomed. Pharmacother. 104: 94-101. https://doi.org/10.1016/j.biopha.2018.05.015
  33. To KK, Tong CW, Wu M, Cho WC. 2018. MicroRNAs in the prognosis and therapy of colorectal cancer: from bench to bedside. World J. Gastroenterol. 24: 2949-2973. https://doi.org/10.3748/wjg.v24.i27.2949
  34. Zhou C, Kong W, Ju T, Xie Q, Zhai L. 2020. MiR-185-3p mimic promotes the chemosensitivity of CRC cells via AQP5. Cancer Biol. Ther. 21: 790-798. https://doi.org/10.1080/15384047.2020.1761238
  35. Ma J, Zhang L, Shang A, Song H, Huo J, Zhang M, et al. 2020. LINC02163 promotes colorectal cancer progression via miR-511-3p/AKT3 axis. Artif. Cells Nanomed. Biotechnol. 48: 961-968. https://doi.org/10.1080/21691401.2020.1773486
  36. Yu X, Xia J, Cao Y, Tang L, Tang X, Li Z. 2020. SNHG1 represses the anti-cancer roles of baicalein in cervical cancer through regulating miR-3127-5p/FZD4/Wnt/beta-catenin signaling. Exp. Biol. Med. 246: 20-30.

Cited by

  1. A Novel Prognostic Prediction Model for Colorectal Cancer Based on Nine Autophagy-Related Long Noncoding RNAs vol.11, 2021, https://doi.org/10.3389/fonc.2021.613949