Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Development of a Novel Cell Surface Attachment System to Display Multi-Protein Complex Using the Cohesin-Dockerin Binding Pair

  • Ko, Hyeok-Jin (Food Biotech R&D Center, Samyang Corp.) ;
  • Song, Heesang (Department of Biochemistry and Molecular Biology, Chosun University School of Medicine) ;
  • Choi, In-Geol (Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University)
  • Received : 2021.05.18
  • Accepted : 2021.07.05
  • Published : 2021.08.28
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Abstract

Autodisplay of a multimeric protein complex on a cell surface is limited by intrinsic factors such as the types and orientations of anchor modules. Moreover, improper folding of proteins to be displayed often hinders functional cell surface display. While overcoming these drawbacks, we ultimately extended the applicability of the autodisplay platform to the display of a protein complex. We designed and constructed a cell surface attachment (CSA) system that uses a non-covalent protein-protein interaction. We employed the high-affinity interaction mediated by an orthogonal cohesin-dockerin (Coh-Doc) pair from Archaeoglobus fulgidus to build the CSA system. Then, we validated the orthogonal Coh-Doc binding by attaching a monomeric red fluorescent protein to the cell surface. In addition, we evaluated the functional anchoring of proteins fused with the Doc module to the autodisplayed Coh module on the surface of Escherichia coli. The designed CSA system was applied to create a functional attachment of dimeric α-neoagarobiose hydrolase to the surface of E. coli cells.

Keywords

Acknowledgement

This study was supported by a grant from Chosun University (2021).

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