대한방사성의약품학회지 (Journal of Radiopharmaceuticals and Molecular Probes)

  • 제7권2호
  • /
  • Pages.113-118
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  • 2021
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  • 2384-1583(pISSN)
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  • 2508-3848(eISSN)
대한방사성의약품학회 (Korean Society of Radiopharmaceuticals and Molecular Probes)
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Study on radiolabeling method of chitosan to improve the radiolabeling yield of the final product

  • Jung Ae Kang (Laboratory Animal Center, Osong Medical Innovation Foundation) ;
  • A-Ram Yu (Laboratory Animal Center, Osong Medical Innovation Foundation) ;
  • Jae Jun Lee (Laboratory Animal Center, Osong Medical Innovation Foundation) ;
  • Yeong Su Ha (Laboratory Animal Center, Osong Medical Innovation Foundation)
  • 투고 : 2021.12.21
  • 심사 : 2021.12.28
  • 발행 : 2021.12.30
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초록

Chitosan is a polysaccharide derived from chitin by deacetylation. Chitosan is non-toxic, biodegradable, and biocompatible, so that it can be used in wide variety of medical applications such as wound healing and antimicrobial biomaterials. It also used as dermal fillers due to its ability to inject with liquid formulations. For investigation on in vivo distribution of these chitosans, Bolton-Hunter-conjugated chitosan (Chitosan-BH) was synthesized by the reaction between the primary amino group of chitosan and N-hydroxysuccinimide ester group of Bolton-Hunter reagent. Then Chitosan-BH was radiolabeled with 125I (Chitosan-BH-125I) using a Chloramine-T method. The effects of each radiolabeling step on the radiolabeling yield of the final product were tested. The results showed that purification step had significant effects on the radiolabeling yield of the final product. Finally, SPECT/CT images were obtained to evaluate in vivo uptake of the radiolabeled chitosan (Chitosan-BH-125I) in several organs. The highest uptake was found in the site of injection at 21 days post-injection. The results of this study suggest that chitosan is expected to be useful for biomaterials of dermal fillers.

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과제정보

이 연구는 보건복지부 첨단의료복합단지 미래의료산업 원스톱 지원 사업(HI19C0763020021)의 지원을 받아 수행하였으며, 다른 이해 관계는 없음을 밝힙니다.

참고문헌

  1. Chandy T, Sharma CP. Chitosan - as a biomaterial. Biomater Artif Cells Artif Organs 1990;18:1-24. https://doi.org/10.3109/10731199009117286
  2. Rinaudo M. Chitin and chitosan: properties and applications. Prog Polym Sci 2006;31:603-632. https://doi.org/10.1016/j.progpolymsci.2006.06.001
  3. Foster LJR, Ho S, Hook J, Basuki M, Marcal H. Chitosan as a Biomaterial: Influence of Degree of Deacetylation on Its Physiochemical, Material and Biological Properties. PLoS One 2015;10: e0135153.
  4. Phillippart C, Hendrickx B, Rharbaoui S, Natalizio A, Boisnic S, Micheels P, Gautier S, Douette P, Hermitte L. Safety and efficacy of a carboxymethyl chitosan dermal injection device for the treatment of skin defects: a first-in-man, pilot, comparative, split-body study. Eur J Dermatol 2021;31:549-558. https://doi.org/10.1684/ejd.2021.4091
  5. Abduljabbar MH, Basendwh MA. Complications of hyaluronic acid fillers and their managements. J Dermatol Dermatol Surg 2016;20:100-106. https://doi.org/10.1016/j.jdds.2016.01.001
  6. Halimi C, Montembault A, Guerry A, Delair T, Viguier E, Fulchiron R, David L. Chitosan solutions as injectable systems for dermal filler applications: rheological characterization and biological evidence. Annu Int Conf IEEE Eng Med Biol Soc 2015;2015:2596-2599.
  7. Blankenberg FG, Strauss HW. Nuclear Medicine Applications in Molecular Imaging. J Magn Reson Imaging 2002;16:352-361. https://doi.org/10.1002/jmri.10171
  8. Khalil MM, Tremoleda JL, Bayomy TB, Gsell W. Molecular SPECT Imaging: An Overview. Int J Mol Imaing 2011;2011:796025.
  9. McFarlane AS. Efficient trace-labelling of proteins with iodine. Nature 1958;182:53.
  10. Rinaudo M, Pavlov G, Desbrieres J. Influence of acetic acid concentration on the solubilization of chitosan. Polymer 1999;40:7029-7032. https://doi.org/10.1016/S0032-3861(99)00056-7
  11. Cunha-Reis C, El Haj AJ, Yang X, Yang Y. Fluorescent labeling of chitosan for use in non-invasive monitoring of degradation in tissue engineering. J Tissue Eng Regen Med 2013;7:39-50. https://doi.org/10.1002/term.494
  12. Furuike T, Komoto D, Hashimoto H, Tamura H. Preparation of chitosan hydrogel and its solubility in organic acids. Int J Biol Macromol 2017;104:1620-1625. https://doi.org/10.1016/j.ijbiomac.2017.02.099
  13. Hermanson GT. Bioconjugate Techniques. 3rd ed. Amsterdam: Elsevier Sience; 2013. p. 1200.
  14. Bolton AE, Hunter WM. The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Biochem J 1973;133:529-539. https://doi.org/10.1042/bj1330529
  15. Sogias IA, Khutoryanskiy VV, Williams AC. Exploring the factors affecting the solubility of chitosan in water. Macromol Chem Phys 2010;211:426-433. https://doi.org/10.1002/macp.200900385
  16. Sun H, Liu XY, Zhu D, Gao D, Ding M. Preparation and reaction of fluorescently-labeled chitosan nano particles. Asian J Chem 2013;25:5658-5660. https://doi.org/10.14233/ajchem.2013.OH52
  17. Chung JK. Sodium iodide symporter: its role in nuclear medicine. J Nucl Med 2002;43:1188-1200.