Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Study on the Correlation between the Soil Properties and Albiflorin, Paeoniflorin Contents of Paeonia lactiflora Pall.

작약의 Albiflorin, Paeoniflorin 함량과 토양특성 간의 상관관계 연구

  • Eo, Hyun Ji (Forest Medicinal Research Center, National Institute of Forest Science) ;
  • Park, Youngki (Forest Medicinal Research Center, National Institute of Forest Science) ;
  • Park, Gwang Hun (Forest Medicinal Research Center, National Institute of Forest Science) ;
  • Kim, Ji-Ah (Forest Medicinal Research Center, National Institute of Forest Science) ;
  • Kim, Da Som (Forest Medicinal Research Center, National Institute of Forest Science) ;
  • Kang, Yeongyeong (Forest Medicinal Research Center, National Institute of Forest Science) ;
  • Kim, Kiyoon (Forest Medicinal Research Center, National Institute of Forest Science) ;
  • Jang, Jun Hyuk (Forest Medicinal Research Center, National Institute of Forest Science) ;
  • Kim, Hyun-Jun (Forest Medicinal Research Center, National Institute of Forest Science)
  • 어현지 (국립산림과학원 산림약용자원연구소) ;
  • 박영기 (국립산림과학원 산림약용자원연구소) ;
  • 박광훈 (국립산림과학원 산림약용자원연구소) ;
  • 김지아 (국립산림과학원 산림약용자원연구소) ;
  • 김다솜 (국립산림과학원 산림약용자원연구소) ;
  • 강연경 (국립산림과학원 산림약용자원연구소) ;
  • 김기윤 (국립산림과학원 산림약용자원연구소) ;
  • 장준혁 (국립산림과학원 산림약용자원연구소) ;
  • 김현준 (국립산림과학원 산림약용자원연구소)
  • Received : 2021.06.01
  • Accepted : 2021.06.28
  • Published : 2021.08.01
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Abstract

The aim of this study was to investigate the correlation between soil properties and marker compounds contents of Paeonia lactiflora. The methods of determining marker compounds were validated by measuring the linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy and recovery using UPLC analysis. P. lactiflora contained albiflorin at 0.04 ± 0.00 ~ 2.79 ± 0.21%, paeoniflorin at 1.98 ± 0.14 ~ 6.67 ± 0.84%. The root dry weight (RDW) of P. lactiflora was 0.06 ± 0.02 ~ 1.27 ± 0.28 kg. The soil properties analysis such as soil pH, electric conductivity (EC), organic matter (OM), total nitrogen (TN), available phosphate (Avail. P2O5), exchangeable cation and cation exchange capacity (CEC) were performed following standard analysis manual. The results of correlation analysis between soil properties and growth characteristics, available P2O5 was positively coreelated with the RDW of P. lactiflora. On the other hand, the RDW of P. lactiflora showed significantly negative correlation with contents of albiflorin and paeoniflorin. The results of this study was might be help to provide useful information on the establish of standard cultivation by the investigate correlation analysis between growth characteristics and marker compound contents of P. lactiflora.

본 연구는 작약의 재배지별 토양특성과 albiflorin, paeoniflorin 함량 간의 상관관계를 구명하기 위하여 수행되었다. UPLC를 사용하여 직선성, LOD, LOQ, 정밀성, 정확성, 회수율을 평가하여 분석법을 검증하였다. 작약의 albiflorin 함량은 0.04 ± 0.00 ~ 2.79 ± 0.21%, paeoniflorin은 1.98 ± 0.14 ~ 6.67 ± 0.84%로 확인되었다. 또한 작약의 개체별 뿌리 중량을 조사한 결과, 0.06 ± 0.02 ~ 1.27 ± 0.28 kg로 지역별 차이를 확인하였다. 작약 재배지의 토양특성은 pH, 전기전도도, 유기물 함량, 질소전량, 유효인산, 치환성 칼륨, 치환성 칼슘, 치환성 마그네슘, 치환성 나트륨, 양이온치환용량 등을 분석하였다. 재배지 토양특성과 작약 뿌리 중량 간의 상관관계를 분석한 결과, 토양의 유효인산과 작약 뿌리 중량 간의 유의적인 정의 상관관계를 확인하였다. 또한 작약 뿌리 중량과 지표성분 함량 간의 상관관계를 분석한 결과, 작약 뿌리 중량은 지표성분인 albiflorin, paeoniflorin의 함량과 유의적인 부의 상관관계를 보이는 것을 확인하였다. 따라서 작약 재배에 있어 지표성분과 생산량을 동시에 증가시키는 재배법 개발에 유용한 정보를 제공할 것으로 판단된다.

Keywords

Acknowledgement

본 연구는 국립산림과학원 일반연구사업 "유용 산림약용자원의 이용 활성화를 위한 천연물지도 작성 연구(Project No. FP0400-2017-03)"의 지원에 의해 이루어진 결과로 이에 감사드립니다.

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