한국자원식물학회지 (Korean Journal of Plant Resources)

  • 제34권2호
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  • Pages.186-196
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  • 2021
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  • 1226-3591(pISSN)
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  • 2287-8203(eISSN)
한국자원식물학회 (The Plant Resources Society of Korea)
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Rhodosporidium toruloides에서의 적색 파프리카 에탄올 추출물의 중성지방 억제 효과

Inhibitory Effects of Ethanol Extract of Red Sweet Pepper (Capsicum annuum L.) on Triglyceride Biosynthesis in Rhodosporidium toruloides

  • 강주원 (전북대학교 한약자원학과) ;
  • 이승제 (전라북도생물산업진흥원) ;
  • 안병용 (전북대학교 한약자원학과)
  • Kang, Ju-Won (Department of Oriental Medicine Resources, Chonbuk National University) ;
  • Lee, Seung-Je (Research and Development Headquaters, Jeonbuk Institute for Food-Bioindustry) ;
  • Ahn, Byung-Yong (Department of Oriental Medicine Resources, Chonbuk National University)
  • 투고 : 2021.01.07
  • 심사 : 2021.03.13
  • 발행 : 2021.04.01
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초록

본 연구는 유지효모 R. toruloides를 활용하여 적색 파프리카 추출물의 중성지방 억제 효과를 확인하기 위하여 수행되었다. 대조구, 양성대조구, 0.02, 0.1 및 0.5% 적색 파프리카 70% 에탄올 추출물을 첨가한 실험구의 총 지질 함량은 각각 80.45±2.40, 86.61±11.58, 79.82±2.80, 85.30±0.91 및 92.40±12.40 mg/g으로 나타났으며, 유지효모의 중성지방 함량은 각각9.89±0.36, 7.58±1.11, 7.28±0.17, 6.68±0.35 및 5.23±0.67 mg/dL으로 나타났다. 대조구, 양성대조구, 0.02, 0.1 및 0.5% 적색 파프리카 70% 에탄올 추출물을 첨가한 실험구의 지방소적 형성을 관찰한 결과, 적색 파프리카 70% 에탄올 추출물의 첨가농도가 증가함에 따라 대조구에 비해 소량의 형광이 관찰되는 것으로 보아 유지효모 내 생성되는 지방소적이 감소됨을 확인하였다. 대조구, 양성대조구, 0.02, 0.1 및 0.5% 적색 파프리카 70% 에탄올 추출물을 첨가한 실험구의 세포수는 각각 1.90±0.16, 2.17±0.25, 1.85±0.11, 2.28±0.21 및 2.49±0.03×108 cell/mL으로 나타났으며, 유리지방산의 함량은 각각2.44±0.05, 2.71±0.07, 2.46±0.16, 2.52±0.10 및 2.98±0.14 mg/g으로 나타났다. 본 연구결과에서 적색 파프리카 70% 에탄올 추출물의 첨가농도에 따른 유지효모 내 중성지방 억제 효과, 지방소적의 감소, 세포수 및 유리지방산의 함량이 증가한 결과를 통해 적색 파프리카 70% 에탄올 추출물이 중성지방 억제 효과에 유의적인 영향을 미치는 것으로 확인되었으며, 추후 활성 물질의 규명에 관한 추가적인 연구가 필요할 것으로 사료된다.

In this study, we investigated inhibitory effect of red sweet pepper (Capsicum annuum L.) on triglyceride biosynthesis in Rhodosporidium toruloides. There was no significant difference in the total lipid content of all the experimental groups including 0.02, 0.1 and 0.5% (w/v) of red sweet pepper 70% ethanol extract treatment (RSPE). However, the triglyceride content was significantly decreased in RSPE group campared to the control group. When the formation of lipid droplet in the oleaginous yeast was examined, a small amount of fluorescence was observed compared to the control as the concentration of RSPE increased. The number of cells and free fatty acid increased in a concentration-dependent manner. These results suggest that RSPE has an anti-obesity effect.

키워드

참고문헌

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