Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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Laboratory model to evaluate efficacy of an experimental titanium oxide nanofibers bleaching agent

  • Clayton Tran (Division of General Dentistry, Loma Linda University School of Dentistry) ;
  • Ellin Choi (Division of General Dentistry, Loma Linda University School of Dentistry) ;
  • Brittany Watu (Division of General Dentistry, Loma Linda University School of Dentistry) ;
  • Udochukwu Oyoyo (Division of General Dentistry, Loma Linda University School of Dentistry) ;
  • Christopher Perry (Department of Biochemistry, Loma Linda University School of Medicine) ;
  • So Ran Kwon (Division of General Dentistry, Loma Linda University School of Dentistry)
  • Received : 2021.01.25
  • Accepted : 2021.05.09
  • Published : 2021.11.30
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Abstract

Objectives: This study aimed to use a laboratory model to evaluate the efficacy of an experimental bleaching agent. Materials and Methods: The model used human extracted molars that were treated and measured for bleaching efficacy. Teeth (n = 50) were distributed into 5 groups: Negative control (NC): immersion in water for 8 hours; Nanofibers (NFs): Experimental titanium dioxide nanofibers with stirring and light activation for 8 hours; Whitestrips (WS): Crest 3D White Glamorous White Whitestrips, 2 applications daily for 30 minutes, 14 days; 1% hydrogen peroxide (HP) standard: 1% hydrogen peroxide for 8 hours; and 30% HP standard: 30% hydrogen peroxide for 8 hours. Instrumental measurements were performed using a spectrophotometer. Results were recorded at baseline, 1-day post-bleaching, and 1-week post-bleaching. Kruskal-Wallis procedure was used to determine differences in color change. Pearson correlation was used to evaluate the relationship between visual and instrumental measurements. Tests of hypotheses were 2-sided with alpha = 0.05. Results: There was no significant difference in color parameters (L1, a1, b1, and shade guide units [SGU]) at baseline (p > 0.05). There was a significant difference among the groups for overall color change (ΔE*ab) and change in shade guide units (ΔSGU) at 1-day and 1-week post-bleaching (p < 0.05). The higher the HP concentration, the higher the color change as expressed in ΔSGU and ΔE*ab. The negative control exceeded the perceptibility threshold of ΔE* = 1.2 regardless of time point. NFs showed a decrease in chroma, but were not statistically different compared to the negative control. Conclusions: The laboratory model was successful in screening an experimental bleaching agent.

Keywords

Acknowledgement

The study was supported by Loma Linda University School of Dentistry Student Research Program.

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