Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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Biological assessment of a new ready-to-use hydraulic sealer

  • Francine Benetti (Department of Restorative Dentistry, Universidade Federal de Minas Gerais (UFMG), School of Dentistry) ;
  • Joao Eduardo Gomes-Filho (Department of Preventive and Restorative Dentistry, Sao Paulo State University (UNESP), School of Dentistry) ;
  • India Olinta de Azevedo-Queiroz (Department of Preventive and Restorative Dentistry, Sao Paulo State University (UNESP), School of Dentistry) ;
  • Marina Carminatti (Department of Preventive and Restorative Dentistry, Sao Paulo State University (UNESP), School of Dentistry) ;
  • Leticia Citelli Conti (Department of Preventive and Restorative Dentistry, Sao Paulo State University (UNESP), School of Dentistry) ;
  • Alexandre Henrique dos Reis-Prado (Department of Restorative Dentistry, Universidade Federal de Minas Gerais (UFMG), School of Dentistry) ;
  • Sandra Helena Penha de Oliveira (Department of Basic Science, Sao Paulo State University (UNESP), School of Dentistry) ;
  • Edilson Ervolino (Department of Basic Science, Sao Paulo State University (UNESP), School of Dentistry) ;
  • Eloi Dezan-Junior (Department of Preventive and Restorative Dentistry, Sao Paulo State University (UNESP), School of Dentistry) ;
  • Luciano Tavares Angelo Cintra (Department of Preventive and Restorative Dentistry, Sao Paulo State University (UNESP), School of Dentistry)
  • Received : 2020.04.01
  • Accepted : 2020.05.29
  • Published : 2021.05.31
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Abstract

Objectives: This study compared the cytotoxicity, biocompatibility, and tenascin immunolabeling of a new ready-to-use hydraulic sealer (Bio-C Sealer) with MTA-Fillapex and white MTA-Angelus. Materials and Methods: L929 fibroblasts were cultivated and exposed to undiluted and diluted material extracts. Polyethylene tubes with or without (the control) the materials were implanted into the dorsa of rats. At 7 days and 30 days, the rats were euthanized, and the specimens were prepared for analysis; inflammation and immunolabeling were measured, and statistical analysis was performed (p < 0.05). Results: MTA-Fillapex exhibited greater cytotoxicity than the other materials at all time points (p < 0.05). The undiluted Bio-C Sealer exhibited greater cytocompatibility at 6 and 48 hours than white MTA-Angelus, with higher cell viability than in the control (p < 0.05). White MTA-Angelus displayed higher cell viability than the control at 24 hours, and the one-half dilution displayed similar results at both 6 and 48 hours (p < 0.05). At 7 days and 30 days, the groups exhibited moderate inflammation with thick fibrous capsules and mild inflammation with thin fibrous capsules, respectively (p > 0.05). At 7 days, moderate to strong immunolabeling was observed (p > 0.05). After 30 days, the control and MTA-Fillapex groups exhibited strong immunolabeling, the white MTA-Angelus group exhibited moderate immunolabeling (p > 0.05), and the Bio-C Sealer group exhibited low-to-moderate immunolabeling, differing significantly from the control (p < 0.05). Conclusions: Bio-C Sealer and white MTA-Angelus exhibited greater cytocompatibility than MTA-Fillapex; all materials displayed adequate biocompatibility and induced tenascin immunolabeling.

Keywords

Acknowledgement

This research received support from the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq (305969/2015-3), Sao Paulo, SP, Brazil.

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