Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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Biocompatibility and bioactive potential of the NeoMTA Plus endodontic bioceramic-based sealer

  • Roberto Alameda Hoshino (Department of Restorative Dentistry, Dental School, Sao Paulo State University (UNESP)) ;
  • Mateus Machado Delfino (Department of Restorative Dentistry, Dental School, Sao Paulo State University (UNESP)) ;
  • Guilherme Ferreira da Silva (Pro-Rectory of Research and Post-graduation, School of Dentistry, Universidade Sagrado Coracao (USC)) ;
  • Juliane Maria Guerreiro-Tanomaru (Department of Restorative Dentistry, Dental School, Sao Paulo State University (UNESP)) ;
  • Mario Tanomaru-Filho (Department of Restorative Dentistry, Dental School, Sao Paulo State University (UNESP)) ;
  • Estela Sasso-Cerri (Department of Morphology, Genetics, Orthodontics and Pediatric Dentistry, Laboratory of Histology and Embryology, Dental School, Sao Paulo State University (UNESP)) ;
  • Paulo Sergio Cerri (Department of Morphology, Genetics, Orthodontics and Pediatric Dentistry, Laboratory of Histology and Embryology, Dental School, Sao Paulo State University (UNESP))
  • Received : 2020.04.03
  • Accepted : 2020.05.11
  • Published : 2021.02.28
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Abstract

Objectives: This study evaluated the biocompatibility and bioactive potential of NeoMTA Plus mixed as a root canal sealer in comparison with MTA Fillapex. Materials and Methods: Polyethylene tubes filled with NeoMTA Plus (n = 20), MTA Fillapex (n = 20), or nothing (control group, CG; n = 20) were inserted into the connective tissue in the dorsal subcutaneous layer of rats. After 7, 15, 30 and 60 days, the specimens were processed for paraffin embedding. The capsule thickness, collagen content, and number of inflammatory cells (ICs) and interleukin-6 (IL-6) immunolabeled cells were measured. von Kossa-positive structures were evaluated and unstained sections were analyzed under polarized light. Two-way analysis of variance was performed, followed by the post hoc Tukey test (p ≤ 0.05). Results: At 7 days, the capsules around NeoMTA Plus and MTA Fillapex had more ICs and IL-6-immunostained cells than the CG. However, at 60 days, there was no significant difference in the IC number between NeoMTA Plus and the CG (p = 0.1137) or the MTA Fillapex group (p = 0.4062), although a greater number of IL-6-immunostained cells was observed in the MTA Fillapex group (p = 0.0353). From 7 to 60 days, the capsule thickness of the NeoMTA Plus and MTA Fillapex specimens significantly decreased, concomitantly with an increase in the collagen content. The capsules around root canal sealers showed positivity to the von Kossa stain and birefringent structures. Conclusions: The NeoMTA Plus root canal sealer is biocompatible and exhibits bioactive potential.

Keywords

Acknowledgement

The authors thank Mr. Pedro Sergio Simoes for technical assistance. We thank CAPES (Brazil) for the fellowship grants to Roberto Alameda Hoshino (code 001) and Mateus Machado Delfino (code 001).

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