Molecules and Cells

  • 제44권7호
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  • Pages.468-480
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  • 2021
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
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Ubiquitin D Promotes Progression of Oral Squamous Cell Carcinoma via NF-Kappa B Signaling

  • Song, An (Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital of Nanjing Medical University) ;
  • Wang, Yi (Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital of Nanjing Medical University) ;
  • Jiang, Feng (Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital of Nanjing Medical University) ;
  • Yan, Enshi (Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University) ;
  • Zhou, Junbo (Department of Stomatology, Nanjing Integrated Traditional Chinese and Western Medicine Hospital) ;
  • Ye, Jinhai (Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital of Nanjing Medical University) ;
  • Zhang, Hongchuang (Department of Stomatology, Xuzhou No. 1 Peoples Hospital) ;
  • Ding, Xu (Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital of Nanjing Medical University) ;
  • Li, Gang (Department of Stomatology, Affiliated Hospital of Xuzhou Medical University) ;
  • Wu, Yunong (Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital of Nanjing Medical University) ;
  • Zheng, Yang (Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital of Nanjing Medical University) ;
  • Song, Xiaomeng (Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital of Nanjing Medical University)
  • 투고 : 2020.11.16
  • 심사 : 2021.05.12
  • 발행 : 2021.07.31
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초록

Ubiquitin D (UBD) is highly upregulated in many cancers, and plays a pivotal role in the pathophysiological processes of cancers. However, its roles and underlying mechanisms in oral squamous cell carcinoma (OSCC) are still unclear. In the present study, we investigated the role of UBD in patients with OSCC. Quantitative real-time polymerase chain reaction and Western blot were used to measure the expression of UBD in OSCC tissues. Immunohistochemistry assay was used to detect the differential expressions of UBD in 244 OSCC patients and 32 cases of normal oral mucosae. In addition, CCK-8, colony formation, wound healing and Transwell assays were performed to evaluate the effect of UBD on the cell proliferation, migration, and invasion in OSCC. Furthermore, a xenograft tumor model was established to verify the role of UBD on tumor formation in vivo. We found that UBD was upregulated in human OSCC tissues and cell lines and was associated with clinical and pathological features of patients. Moreover, the overexpression of UBD promoted the proliferation, migration and invasion of OSCC cells; however, the knockdown of UBD exerted the opposite effects. In this study, our results also suggested that UBD promoted OSCC progression through NF-κB signaling. Our findings indicated that UBD played a critical role in OSCC and may serve as a prognostic biomarker and potential therapeutic target for OSCC treatment.

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과제정보

This research was supported by the National Natural Science Foundation of China (81772887). Jiangsu Provincial Medical Innovation Team (CXTDA2017036), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD, 2018-87), Jiangsu Provincial Medical Youth Talent (QNRC2016854), and Natural Science Foundation of Jiangsu Province of China (BK20171488).

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