대한한의학회지 (The Journal of Korean Medicine)

  • 제42권4호
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  • Pages.25-39
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  • 2021
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  • 1010-0695(pISSN)
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  • 2288-3339(eISSN)
대한한의학회 (The Society of Korean Medicine)
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Assessment of genotoxicity of Ssanghwa-tang, an herbal formula, by using bacterial reverse mutation, chromosome aberration, and in vivo micronucleus tests

  • Jang, Ji-Hye (KM Science Research Division, Korea Institute of Oriental Medicine) ;
  • Seo, Chang-Seob (KM Science Research Division, Korea Institute of Oriental Medicine) ;
  • Lee, Mee-Young (KM Convergence Research Division, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (KM Science Research Division, Korea Institute of Oriental Medicine) ;
  • Han, Su-Cheol (Jeonbuk Branch Institute, Korea Institute of Toxicology) ;
  • Ha, Hyekyung (KM Science Research Division, Korea Institute of Oriental Medicine)
  • 투고 : 2021.01.28
  • 심사 : 2021.11.05
  • 발행 : 2021.12.01
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초록

Objectives: Ssanghwa-tang (SHT) is a traditional herbal formula comprising nine medicinal herbs, and it is used for reducing fatigue in Korea. SHT exerts various effects such as anti-inflammatory, antioxidant, and anti-aging activities, and protection against acute hepatotoxicity. However, the genotoxicity of SHT has not yet been established. Methods: Ten components were identified in SHT water extract by using high-performance liquid chromatography analysis. We assessed the genotoxicity of SHT by using bacterial reverse mutation (Ames test), chromosome aberration, and in vivo micronucleus tests. Results: The contents of paeoniflorin, glycyrrhizin, and liquiritin apioside in SHT were 15.57, 6.94, and 3.48 mg/g extract, respectively. SHT did not increase the revertant colonies of Salmonella typhimurium and Escherichia coli strains in the presence or absence of metabolic activity. Although SHT did not induce structurally abnormal chromosomes in Chinese hamster lung (CHL) cells in the presence of metabolic activity, the number of structurally aberrated chromosomes increased dose-dependently in the absence of metabolic activity. In the in vivo micronucleus test, SHT did not affect the formation of micronuclei compared with the vehicle control. Conclusions: Genotoxicity of SHT was not observed in the Ames test and in vivo micronucleus test. However, based on the results of chromosome aberration test, it can be presumed that SHT has the potential to induce genotoxicity because it induced structurally abnormal chromosomes in the absence of metabolic activity.

키워드

과제정보

This research was supported by grants of the 'Construction of Scientific Evidence for Herbal Medicine Formulas (K17251)' and 'Construction of Safety and Efficacy for Traditional Herbal Prescriptions of Medicinal Institution (KSN2013310)' projects from the Korea Institute of Oriental Medicine (KIOM), Republic of Korea. We would like to thank the Korea Institute of Toxicology (KIT) for providing animal care facility for the study.

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