Journal of Plant Biotechnology

  • 제48권2호
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  • Pages.86-92
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  • 2021
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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Initiation, proliferation, and improvement of a micropropagation system for mass clonal production of banana through shoot-tip culture

  • Khaskheli, Allah Jurio (Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University) ;
  • Ali, Muharam (Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University) ;
  • Shah, Syad Zakir Hussain (Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University) ;
  • Memon, Zohra Fatima (Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University) ;
  • Awan, Saleem (Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University) ;
  • Khaskheli, Muhammad Ibrahim (Department of Plant Protection, Faculty of Crop Protection, Sindh Agriculture University) ;
  • Khaskheli, Mohsin Ali (Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University) ;
  • Magsi, Bilqees (Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University) ;
  • Qambrani, Zareen (Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University) ;
  • Khaskheli, Asad Ali (Department of Animal Nutrition, Shaheed Benazir Bhutto University of Veterinary and Animal Sciences)
  • 투고 : 2021.03.22
  • 심사 : 2021.04.16
  • 발행 : 2021.06.30
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초록

The present study aimed to evaluate the initiation, proliferation potential, and mass clonal production ability of a micropropagation system for banana through tissue culture. A total of 60 explants were cultured on basal media supplemented with various concentrations of BAP and NAA. Banana plants regenerated on MS basal medium (control) without the addition of BAP + NAA showed a significantly (P < 0.05) lower survival rate with no signs of shoots up to the end of the experimental period. The results further revealed that the performance in MSS-XI medium was almost 89%, followed by MSS-IX and MSS-X media, both of which showed performance up to 88%. In contrast, the performance in the MSS-XVI medium was less than 60%, at the less duration of time and highly shoot induction detected at MSS-XIII medium. The maximum number of shoots (4.9) was observed in the medium supplemented with growth adjuster MSS-XI, followed by the MSS-XII medium (4.5). Surprisingly, the best performance was observed for the MSR-VII medium approximately 16 days after initiation, while the lowest performance was observed with MSR-XI (approximately 31 days). The maximum rooting percentage (98%) was observed in the MSR-V to MSR-VIII media (98%), while the minimum rooting percentage was observed in MSR-XI (approximately 45%).

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과제정보

Authors very grateful and would like to thank School of Life Sciences, Lanzhou University, Gansu Province, P.R. China for provided me an opportunity for conducting Post doctorate program. And also would like to thank Department of Biotechnology, Sindh Agriculture University, Tandojam for hosted me the employment opportunity and gave me background. The present work was supported by the Higher Education Commission of Pakistan, Funded Start-up Research Grant Program [grant No. 4821].

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