Physical Therapy Rehabilitation Science

korean Academy of Physical Therapy Rehabilitation Science (물리치료재활과학회)
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Effects of Red Ginseng on Exercise Capacity and Peripheral Fatigue in Mice

  • Kim, Daehyun (Department of Chemistry Life Science, Sahmyook University) ;
  • Lee, Byounggwan (Department of Chemistry Life Science, Sahmyook University) ;
  • Kim, Heejin (Uimyung Research Institute for Neuroscience, Sahmyook University) ;
  • Kim, Mikyung (Department of Chemistry Life Science, Sahmyook University)
  • Received : 2021.06.06
  • Accepted : 2021.06.18
  • Published : 2021.06.30
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Abstract

Objective: Fatigue can decrease both quality of life and work efficiency. Ginseng is one of the most popular herbal treatments for improving personal health, with applications in treating fatigue. However, the exact mechanisms of anti-fatigue effects are still unclear. Thus, we investigated the effect of red ginseng powder (RGP) on exercise capacity and peripheral fatigue using both behavioral and molecular experiments in mice. Design: Four-groups behavioral and molecular experiment. Methods: Male 6-weeks-old ICR mice were treated with distilled water, 100, and 200 mg/kg RGP for 5 days via oral administration. The exercise capacity of each animal group was measured by locomotor activity, rota-rod, hanging wire, and cold swimming tests. Additionally, after performing the treadmill to induce fatigue, lactate expression and molecular experiments were investigated using mice gastrocnemius. Results: Mice treated with RGP exhibited increased exercise capacity in the behavioral tests. Additionally, RGP induced a dose-dependent decrease in lactate levels after high-intensity exercise, and Monocarboxylate transporter (MCT) 4 expression increased in groups treated with RGP. However, there was no significant change in MCT1. Conclusions: These results suggest that RGP exerts several anti-fatigue properties by lower lactate and improved exercise capacity. Increased MCT4 expression may also affect lactate transport. Thus, this study suggests that the anti-fatigue properties of RGP might be associated with MCT4 activity.

Keywords

Acknowledgement

This study was supported by the National Research Foundation (NRF) funded by the Korea government (NRF-2019R1A6A3A01095209).

References

  1. Schwartz JE, Jandorf L, Krupp LB. The measurement of fatigue: a new instrument. J Psychosom Res. 1993;37:753-62. https://doi.org/10.1016/0022-3999(93)90104-N
  2. Afari N, Buchwald D. Chronic fatigue syndrome: a review. Am J Psychiatry. 2003;160:221-36. https://doi.org/10.1176/appi.ajp.160.2.221
  3. Pawlikowska T, Chalder T, Hirsch SR, Wallace P, Wright DJ, Wessely SC. Population based study of fatigue and psychological distress. BMJ. 1994;308:763-6. https://doi.org/10.1136/bmj.308.6931.763
  4. Enoka RM. Muscle fatigue - from motor units to clinical symptoms. J Biomech. 2012;45:427-33. https://doi.org/10.1016/j.jbiomech.2011.11.047
  5. Paillard T. Effects of general and local fatigue on postural control: A review. Neurosci Biobehav Rev. 2012;36:162-76. https://doi.org/10.1016/j.neubiorev.2011.05.009
  6. Josef F. Biomarkers of peripheral muscle fatigue during exercise. BMC Musculoskelet Disord. 2012;13:1-13. https://doi.org/10.1186/1471-2474-13-1
  7. Enoki T, Yoshida Y, Hatta H, Bonen A. Exercise training alleviates MCT1 and MCT4 reductions in heart and skeletal muscles of STZ-induced diabetic rats. J Appl Physiol. 2003;94:2433-8. https://doi.org/10.1152/japplphysiol.01155.2002
  8. Halestrap AP, Price NT. The proton-linked monocarboxylate transporter (MCT) family: structure, function and regulation. Biochem J. 1999;343:281-99. https://doi.org/10.1042/bj3430281
  9. Bonen A. The expression of lactate transporters (MCT1 and MCT4) in heart and muscle. Eur J Appl Physiol. 2001;86:6-11. https://doi.org/10.1007/s004210100516
  10. Wang CZ, Yuan CS. Potential role of Ginseng in the treatment of colorectal cancer. Am J Chin Med. 2008;36:1019-28. https://doi.org/10.1142/S0192415X08006545
  11. Arring NM, Millstine D, Marks LA, Nail LM. Ginseng as a Treatment for Fatigue: A Systematic Review. J Altern Complement Med. 2018;24:624-33. https://doi.org/10.1089/acm.2017.0361
  12. Baek KS, Yi YS, Son YJ, Yoo S, Sung NY, Kim Y, et al. In vitro and in vivo anti-inflammatory activities of Korean red Ginseng-derived components. J Ginseng Res. 2016;40:437-44. https://doi.org/10.1016/j.jgr.2016.08.003
  13. Ray MA, Trammell RA, Verhulst S, Ran S, Toth LA. Development of a mouse model for assessing fatigue during chemotherapy. Comp Med. 2011;61:119-30.
  14. Thomas C, Bishop D, Moore-Morris T, Mercier J. Effects of high-intensity training on MCT1, MCT4, and NBC expressions in rat skeletal muscles: influence of chronic metabolic alkalosis. Am J Physiol Endocrinol Metab. 2007;293:E916-22.
  15. Bharti M, Fudong L, Yan X, Rebecca P, Jun L, Louise M. Functional recovery in aging mice after experimental stroke. Brain Behav Immun. 2011;25:1689-700. https://doi.org/10.1016/j.bbi.2011.06.015
  16. Gosker HR, Schols AMWJ. Fatigued muscles in COPD but no finishing line in sight. Eur Respir J. 2008;31:693-4. https://doi.org/10.1183/09031936.00015308
  17. Rainoldi A, Gazzoni M, Merletti R, Minetto MA. Mechanical and EMG responses of the vastus lateralis and changes in biochemical variables to isokinetic exercise in endurance and power athletes. J Sports Sci. 2008;26:311-9.
  18. Ohba T, Domoto S, Tanaka M, Nakamura S, Shimazawa M, Hara H. Myalgic encephalomyelitis/chronic fatigue syndrome induced by repeated forced swimming in mice. Biol Pharm Bull. 2019;42:1140-5. https://doi.org/10.1248/bpb.b19-00009
  19. Hsiao CY, Hsu YJ, Tung YT, Lee MC, Huang CC, Hsieh CC. Effects of Antrodia camphorata and panax ginseng supplementation on anti-fatigue properties in mice. J Vet Med Sci. 2018;80:284-91. https://doi.org/10.1292/jvms.17-0572
  20. Izquierdo M, Gonzalez-Izal M, Navarro-Amezqueta I, Calbet JAL, Ibanez J, Malanda A, et al. Effects of strength training on muscle fatigue mapping from surface EMG and blood metabolites. Med Sci Sports Exerc. 2011;43:303-11.
  21. Strojnik V, Komi P V. Fatigue after submaximal intensive stretch-shortening cycle exercise. Med Sci Sports Exerc. 2000;32:1314-9. https://doi.org/10.1097/00005768-200007000-00020
  22. White GE, Wells GD. The effect of on-hill active recovery performed between runs on blood lactate concentration and fatigue in alpine ski racers. J Strength Cond Res. 2015;29:800-6. https://doi.org/10.1519/JSC.0000000000000677
  23. Ma GD, Chiu CH, Hsu YJ, Hou CW, Chen YM, Huang CC. Changbai mountain ginseng (Panax ginseng C.A. Mey) extract supplementation improves exercise performance and energy utilization and decreases fatigue-associated parameters in mice. Molecules. 2017;22:237. https://doi.org/10.3390/molecules22020237
  24. Enoki T, Yoshida Y, Lally J, Hatta H, Bonen A. Testosterone increases lactate transport, monocarboxylate transporter (MCT) 1 and MCT4 in rat skeletal muscle. J Physiol. 2006;577:433-43. https://doi.org/10.1113/jphysiol.2006.115436
  25. Juel G, Halestrap AP. Lactate transport in skeletal muscle - Role and regulation of the monocarboxylate transporter. J Physiol. 1999;517:633-42. https://doi.org/10.1111/j.1469-7793.1999.0633s.x
  26. Coles L, Litt J, Hatta H, Bonen A. Exercise rapidly increases expression of the monocarboxylate transporters MCT1 and MCT4 in rat muscle. J Physiol. 2004;561:253-61. https://doi.org/10.1113/jphysiol.2004.073478
  27. Manning Fox JE, Meredith D, Halestrap AP. Characterisation of human monocarboxylate transporter 4 substantiates its role in lactic acid efflux from skeletal muscle. J Physiol. 2000;529:285-93. https://doi.org/10.1111/j.1469-7793.2000.00285.x
  28. Ullah MS, Davies AJ, Halestrap AP. The plasma membrane lactate transporter MCT4, but not MCT1, is up-regulated by hypoxia through a HIF-1 α-dependent mechanism. J Biol Chem. 2006;281:9030-7. https://doi.org/10.1074/jbc.M511397200
  29. Thomas C, Perrey S, Lambert K, Hugon G, Mornet D, Mercier J. Monocarboxylate transporters, blood lactate removal after supramaximal exercise, and fatigue indexes in humans. J Appl Physiol. 2005;98:804-9. https://doi.org/10.1152/japplphysiol.01057.2004
  30. Thomas C, Bishop DJ, Lambert K, Mercier J, Brooks GA. Effects of acute and chronic exercise on sarcolemmal MCT1 and MCT4 contents in human skeletal muscles: current status. Am J PhysiolRegulIntegr Comp Physiol. 2012;302:R1-14. https://doi.org/10.1152/ajpregu.00250.2011
  31. Kim TW, Park SS, Kim BK, Sim YJ, Shin MS. Effects of sildenafil citrate on peripheral fatigue and exercise performance after exhaustive swimming exercise in rats. J Exerc Rehabil. 2019;15:751-6. https://doi.org/10.12965/jer.1938712.356
  32. Green HJ, Duhamel TA, Holloway GP, Moule JW, Ranney DW, Tupling AR, et al. Rapid upregulation of GLUT-4 and MCT-4 expression during 16 h of heavy intermittent cycle exercise. Am J Physiol RegulIntegr Comp Physiol. 2008;294:R594-600. https://doi.org/10.1152/ajpregu.00699.2007
  33. Bishop D, Edge J, Thomas C, Mercier J. High-intensity exercise acutely decreases the membrane content of MCT1 and MCT4 and buffer capacity in human skeletal muscle. J Appl Physiol. 2007;102:616-21. https://doi.org/10.1152/japplphysiol.00590.2006
  34. Tonouchi M, Hatta H, Bonen A. Muscle contraction increases lactate transport while reducing sarcolemmal MCT4, but not MCT1. Am J Physiol Endocrinol Metab. 2002;282:1062-9.

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