한국동물생명공학회지 (Journal of Animal Reproduction and Biotechnology)

  • 제36권2호
  • /
  • Pages.82-90
  • /
  • 2021
  • /
  • 2671-4639(pISSN)
  • /
  • 2671-4663(eISSN)
한국동물생명공학회 (The Korean Society of Animal Reproduction and Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Improvement of pregnancy rate after deep uterine artificial insemination with frozen-thawed cauda epididymal spermatozoa in Hanwoo cattle

  • Kang, Sung-Sik (Hanwoo Research Institute, National Institute Animal Science, Rural Development Administration) ;
  • Kim, Ui-Hyung (Hanwoo Research Institute, National Institute Animal Science, Rural Development Administration) ;
  • Ahn, Jun Sang (Hanwoo Research Institute, National Institute Animal Science, Rural Development Administration) ;
  • Won, Jeong Il (Hanwoo Research Institute, National Institute Animal Science, Rural Development Administration) ;
  • Cho, Sang-Rae (Hanwoo Research Institute, National Institute Animal Science, Rural Development Administration)
  • 투고 : 2021.05.10
  • 심사 : 2021.05.18
  • 발행 : 2021.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In the present study, we examined if deep uterine artificial insemination (DUAI) can improve the pregnancy rate of artificial insemination (AI) using epididymal spermatozoa (ES) in Hanwoo cattle. The estrus cycles of 88 Hanwoo cows were synchronized, and 17 cows were artificially inseminated using the DUAI method with ES, 20 cows were artificially inseminated via the uterine body (BUAI) method with ES, and as a control, 51 cows were inseminated by using the BUAI method with ejaculated spermatozoa from 1 proven bull after frozen thawing. The pregnancy rate of the DUAI method (58.8%) was higher than that of the BUAI method (25.0%, p = 0.0498). The motility of ES was examined immediately after thawing and after 3 and 6 h of incubation. The rapid progressive sperm motility of the control group was significantly higher than that of the ES group immediately after thawing and after 3 and 6 h of incubation (p < 0.05). The straight line velocity and average path velocity of the ES group after 6 h of incubation were significantly lower than those in the control group (p < 0.05). The linearity and amplitude of lateral head of ES were lower than those at 6 h (p < 0.05). The flagellar beat cross frequency and hyperactivation of ES were lower than the control spermatozoa immediately after thawing and at 3 h (p < 0.05). These motility parameters suggested that ES had a low motility and fertilization ability compared to the control spermatozoa. After frozen-thawing and 3 h of incubation, the percentage of live spermatozoa with intact acrosomes in the ES was significantly lower than that in ejaculated spermatozoa (p < 0.05). Our findings suggested that the DUAI method can overcome the low pregnancy rate of ES, despite the low motility, viability, and fertilization ability of ES.

키워드

과제정보

This study was conducted with the support of the Cooperative Research Program for Agriculture Science & Technology Development project: "Development of improved technologies for preservation of Hanwoo bull semen and technology application", PJ0143252021, RDA, Korea, and the 2021 Postdoctoral Fellowship Program of the Hanwoo Research Institute, NIAS, RDA, Korea. We thank MyungSuk Lee and Seok-Dong Lee at the Hanwoo Research Institute and Min-Hyung Jeon in the In-Woo Animal Hospital, Gochang, Korea for their assistance with epididymal spermatozoa recovery and freezing.

참고문헌

  1. Aitken SC, Lippman ME, Kasid A, Schoenberg DR. 1985. Relationship between the expression of estrogen-regulated genes and estrogen-stimulated proliferation of MCF-7 mammary tumor cells. Cancer Res. 45:2608-2615.
  2. Amann RP, Seidel GE Jr, Mortimer RG. 2000. Fertilizing potential in vitro of semen from young beef bulls containing a high or low percentage of sperm with a proximal droplet. Theriogenology 54:1499-1515. https://doi.org/10.1016/S0093-691X(00)00470-2
  3. Cary JA, Madill S, Farnsworth K, Hayna JT, Duoos L, Fahning ML. 2004. A comparison of electroejaculation and epididymal sperm collection techniques in stallions. Can. Vet. J. 45:35-41.
  4. Chamberland A, Fournier V, Tardif S, Sirard MA, Sullivan R, Bailey JL. 2001. The effect of heparin on motility parameters and protein phosphorylation during bovine sperm capacitation. Theriogenology 55:823-835. https://doi.org/10.1016/S0093-691X(01)00446-0
  5. Chang LB, Chou CJ, Shiu JS, Tu PA, Gao SX, Peng SY, Wu SC. 2017. Artificial insemination of Holstein heifers with sexsorted semen during the hot season in a subtropical region. Trop. Anim. Health Prod. 49:1157-1162. https://doi.org/10.1007/s11250-017-1311-y
  6. Donnelly ET, Lewis SE, McNally JA, Thompson W. 1998. In vitro fertilization and pregnancy rates: the influence of sperm motility and morphology on IVF outcome. Fertil. Steril. 70:305-314. https://doi.org/10.1016/S0015-0282(98)00146-0
  7. Hori T, Ichikawa M, Kawakami E, Tsutsui T. 2004. Artificial insemination of frozen epididymal sperm in beagle dogs. J. Vet. Med. Sci. 66:37-41. https://doi.org/10.1292/jvms.66.37
  8. Hunter RH. 2003. Advances in deep uterine insemination: a fruitful way forward to exploit new sperm technologies in cattle. Anim. Reprod. Sci. 79:157-170. https://doi.org/10.1016/S0378-4320(03)00163-5
  9. Kang SS, Kim UH, Jeon MH, Lee MS, Cho SR. 2018. Comparison of spermatozoa recovery methods on cauda epididymal sperm of Hanwoo bulls. J. Emb. Trans. 33:321-326. https://doi.org/10.12750/JET.2018.33.4.321
  10. Kang SS, Koyama K, Huang W, Yang Y, Yanagawa Y, Takahashi Y, Nagano M. 2015. Addition of D-penicillamine, hypotaurine, and epinephrine (PHE) mixture to IVF medium maintains motility and longevity of bovine sperm and enhances stable production of blastocysts in vitro. J. Reprod. Dev. 61:99-105. https://doi.org/10.1262/jrd.2014-112
  11. Kovacs A and Foote RH. 1992. Viability and acrosome staining of bull, boar and rabbit spermatozoa. Biotech. Histochem. 67:119-124. https://doi.org/10.3109/10520299209110020
  12. Kozdrowski R, Nizanski W, Dubiel A, Olech W. 2011. Possibilities of using the European bison (Bison bonasus) epididymal spermatozoa collected post-mortem for cryopreservation and artificial insemination: a pilot study. Reprod. Biol. Endocrinol. 9:31. https://doi.org/10.1186/1477-7827-9-31
  13. Kunkitti P, Axner E, Bergqvist AS, Sjunnesson Y. 2016. In vitro fertilization using frozen-thawed feline epididymal spermatozoa from corpus and cauda regions. Theriogenology 86:1403-1408. https://doi.org/10.1016/j.theriogenology.2016.04.085
  14. Lopez-Gatius F. 2000. Site of semen deposition in cattle: a review. Theriogenology 53:1407-1414. https://doi.org/10.1016/S0093-691X(00)00283-1
  15. Lopez-Gatius F and Camon-Urgel J. 1988. Increase of pregnancy rate in dairy cattle after preovulatory follicle palpation and deep cornual insemination. Theriogenology 29:1099-1103. https://doi.org/10.1016/S0093-691X(88)80034-7
  16. Martins CF, Rumpf R, Pereira DC, Dode MN. 2007. Cryopreservation of epididymal bovine spermatozoa from dead animals and its uses in vitro embryo production. Anim. Reprod. Sci. 101:326-331. https://doi.org/10.1016/j.anireprosci.2007.01.018
  17. Meirelles C, Kozicki LE, Weiss RR, Segui MS, Souza A, dos Santos IW, Breda JCD. 2012. Comparison between deep intracornual artificial insemination (DIAI) and conventional artificial insemination (AI) using low concentration of spermatozoa in beef cattle. Braz. Arch. Biol. Technol. 55:371-374. https://doi.org/10.1590/S1516-89132012000300006
  18. Morrell JM, Nongbua T, Valeanu S, Lima Verde I, Lundstedt-Enkel K, Edman A, Johannisson A. 2017. Sperm quality variables as indicators of bull fertility may be breed dependent. Anim. Reprod. Sci. 185:42-52. https://doi.org/10.1016/j.anireprosci.2017.08.001
  19. Okazaki T, Akiyoshi T, Kan M, Mori M, Teshima H, Shimada M. 2012. Artificial insemination with seminal plasma improves the reproductive performance of frozen-thawed boar epididymal spermatozoa. J. Androl. 33:990-998. https://doi.org/10.2164/jandrol.111.015115
  20. Seidel GE Jr and Schenk JL. 2008. Pregnancy rates in cattle with cryopreserved sexed sperm: effects of sperm numbers per inseminate and site of sperm deposition. Anim. Reprod. Sci. 105:129-138. https://doi.org/10.1016/j.anireprosci.2007.11.015
  21. Senger PL, Becker WC, Davidge ST, Hillers JK, Reeves JJ. 1988. Influence of cornual insemination on conception in dairy cattle. J. Anim. Sci. 66:3010-3016. https://doi.org/10.2527/jas1988.66113010x
  22. Stauss CR, Votta TJ, Suarez SS. 1995. Sperm motility hyperactivation facilitates penetration of the hamster zona pellucida. Biol. Reprod. 53:1280-1285. https://doi.org/10.1095/biolreprod53.6.1280
  23. Suarez SS and Dai X. 1992. Hyperactivation enhances mouse sperm capacity for penetrating viscoelastic media. Biol. Reprod. 46:686-691. https://doi.org/10.1095/biolreprod46.4.686
  24. Tsutsui T, Wada M, Anzai M, Hori T. 2003. Artificial insemination with frozen epididymal sperm in cats. J. Vet. Med. Sci. 65:397-399. https://doi.org/10.1292/jvms.65.397
  25. Verberckmoes S, Van Soom A, De Pauw I, Dewulf J, Vervaet C, de Kruif A. 2004. Assessment of a new utero-tubal junction insemination device in dairy cattle. Theriogenology 61:103-115. https://doi.org/10.1016/S0093-691X(03)00186-9
  26. Vishwanath R. 2003. Artificial insemination: the state of the art. Theriogenology 59:571-584. https://doi.org/10.1016/S0093-691X(02)01241-4
  27. Waberski D, Meding S, Dirksen G, Weitze KF, Leiding C, Hahn R. 1994. Fertility of long-term-stored boar semen: influence of extender (Androhep and Kiev), storage time and plasma droplets in the semen. Anim. Reprod. Sci. 36:145-151. https://doi.org/10.1016/0378-4320(94)90061-2
  28. Whitfield CH and Parkinson TJ. 1992. Relationship between fertility of bovine semen and in vitro induction of acrosome reactions by heparin. Theriogenology 38:11-20. https://doi.org/10.1016/0093-691X(92)90214-C
  29. Yang BC, Kang SS, Park CS, Kim UH, Kim HC, Jeon GJ, Kim S, Lee SD, Lee HJ, Cho SR. 2015. Motility, fertilizability and subsequent embryonic development of frozen-thawed spermatozoa derived from epididymis in Hanwoo. J. Emb. Trans. 30:271-276. https://doi.org/10.12750/JET.2015.30.4.271
  30. Yulnawati Y, Maheshwari H, Rizal M, Boediono A. 2013. Frozen-thawed epididymal sperm quality and the success rate of artificial insemination in spotted buffaloes (Bubalus bubalis carabanensis). Buffalo Bull. 32(Special Issue 2):494-497.