Fisheries and Aquatic Sciences

한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
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Field experiment on effect of butaphosphan and cyanocobalamin complex on the immunity and stress of olive flounder at low temperature

  • Kim, Seung Min (Mokpo Regional Office, National Fishery Products Quality Management) ;
  • Lee, Da Won (Aquatic Life Disease Control Division, National Fishery Products Quality Management) ;
  • Kim, You Jeong (Central Research Institute, Woogen B&G) ;
  • Jun, Lyu Jin (Department of Aquatic Biomedical Science, Jeju National University) ;
  • Park, Hyun Kyung (Department of Aquatic Biomedical Science, Jeju National University) ;
  • Kim, Ye Ji (Department of Aquatic Biomedical Science, Jeju National University) ;
  • Jeong, You Yong (Central Research Institute, Woogen B&G) ;
  • Lee, Sung Ho (Central Research Institute, Woogen B&G) ;
  • Kwon, Mun Gyeong (Aquatic Life Disease Control Division, National Fishery Products Quality Management) ;
  • Jeong, Joon Bum (Department of Aquatic Biomedical Science, Jeju National University)
  • 투고 : 2021.03.09
  • 심사 : 2021.03.26
  • 발행 : 2021.04.30
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초록

In this study, a fish metabolic accelerator (a combination of butaphosphan and cyanocobalamin [BPC]) was injected into the muscle of the olive flounder, Paralichthys olivaceus, to investigate its effect on immunity and stress in fish maintained at low temperatures. A single dose of BPC was injected (100 mg/kg body weight) into the olive flounder, and its immunity and stress were observed after one and two weeks. Immunity tests revealed the presence of lysozyme (LZM), nitroblue tetrazolium (NBT), myeloperoxidase (MPO), anti-protease (AP), glutathione peroxidase (GPx), and total immunoglobulin (TIg). BPC injection was found to increase immunity activity compared to the control group. In particular, there was significantly high GPx activity. There was similarly high activity for MPO and GPx in the first week following the injection, followed by significant differences between the BPC-injected and control groups in the second week. There was a reduced low water-temperature stress response in the BPC-injected fish, as evidenced by the cortisol and glucose levels of the control and BPC groups. Lower levels were also observed in the BPC group than the control group during the second week. Cortisol levels were significantly lower in the BPC group than the control group. Histological examinations were conducted in the first and second weeks after the intramuscular injection of the recommended BPC dose to confirm the safety of BPC in aquaculture. There were no abnormalities observed in any tissue samples. This study confirms that the injection of BPC is safe even when used in a culture situation. BPC helps relieve stress and improves non-specific immune responses (innate immunity) in the olive flounder.

키워드

과제정보

This work was supported by a grant from the National Fishery Products Quality Management Service and the research grant of Jeju National University in 2020.

참고문헌

  1. Alkobaby AI. Effects of maternal injection with organic phosphorus and vitamin B12 on reproductive performance and newly hatched offspring of Nile tilapia (Oreochromis niloticus). In: Proceedings of the 8th International Symposium on Tilapia in Aquaculture; 2008; Cairo, Egypt. p. 375-86.
  2. Chen D, Ainsworth AJ. Glucan administration potentiates immune defense mechanism of channel catfish, Ictalurus punatatus Rafinesque. J Fish Dis. 1992;15:295-304. https://doi.org/10.1111/j.1365-2761.1992.tb00667.x
  3. Cho Y. Pharmaceutical composition for animal. Korea patent 10-2016-0088486. 2016 Jul 26.
  4. Deniz A, Spiecker-Hauser U, Rhagen M. Efficacy of a butafosfan and vitamin B12 combination (Catosal®) on biochemical and hematological blood parameters in dogs treated with dexamethason. Int J Appl Res Vet Med. 2009;7:116-29.
  5. Ellis AE. Serum anti-protease in fish. In: Stolen JS, Fletcher TC, Anderson DP, Roberson WB, Van Muiswinkel WB, editors. Techniques in fish immunology. Fair Haven, NJ: SOS Publication; 1990. p. 95-9.
  6. EMEA [European Agency for the Evaluation of Medicinal Products]. Committee for Veterinary Medicinal Products: barium selenate. London: EMEA; 1999. Report No.: EMEA/MRL/580/99-FINAL.
  7. EMEA [European Agency for the Evaluation of Medicinal Products]. Committee for Veterinary Medicinal Products: butafosfan. London: EMEA: 2000. Report No.: EMEA/MRL/734/00-FINAL.
  8. EMA [European Medicines Agency]. European Public MRL assessment report (EPMAR): butafosfan (all mammalian food producing species). Amsterdam: EMA; 2014. Report No.: EMA/CVMP/335153/2013.
  9. Fauconneau B, Choubert G, Blanc D, Breque J, Luquet P. Influence of environmental temperature on flow rate of foodstuffs through the gastrointestinal tract of rainbow trout. Aquaculture. 1983;34:27-39. https://doi.org/10.1016/0044-8486(83)90289-2
  10. Flasshoff FH. Clinical and chemical blood serum investigations in cattle and treatment studies with ornithine-aspartate product HMV 20 and with catosal for the reduction of fertility and health disorders [Ph.D. dissertation]. Hannover: Tierarztliche Hochschule Hannover; 1974.
  11. Furll M, Deniz A, Westphal B, Illing C, Constable PD. Effect of multiple intravenous injections of butaphosphan and cyanocobalamin on the metabolism of periparturient dairy cows. J Dairy Sci. 2010;93:4155-64. https://doi.org/10.3168/jds.2009-2914
  12. Furll M, Wittek T, Gegenbach S, Schmidt B. Effects of preoperative application of butafosfan and cyanocobalamin on reconvalescence, clinico-chemical parameters, and antioxidative metabolism and postoperative abormasal emptying in cows with abomasal dislocation. Tierarztl Prax. 2006;34:351-6. https://doi.org/10.1055/s-0038-1625758
  13. Grinde B. Lysozyme from rainbow trout, Salmo gairdneri Richardson, as an antibacterial against fish pathogens. J Fish Dis. 1989;12:92-104. https://doi.org/10.1111/j.1365-2761.1989.tb00281.x
  14. Hasi S, Jiang J, Zhu B. Studies on Anti-heat stress effects and mechanisms of compound butafosfon solution. Acta Vet Zootech Sinic. 2005b;36:1334. https://doi.org/10.3321/j.issn:0366-6964.2005.12.017
  15. Hasi SR, Du X, Zhu B, Jiang J. Studies on effects of compound butaphosphan solution on endurance capability and energy metabolism in mice. Acta Vet Zootech Sinic. 2004;35:290-4. https://doi.org/10.3321/j.issn:0366-6964.2004.03.011
  16. Hasi SR, Jiang J, Du X, Zhu B. Anti-cold stress effects and mechanisms of compound butaphosphan solution. Prog Vet Med. 2005a;26:59-62.
  17. Kang DY, Kang HW, Kim GH, Jo KC, Kim HC. Effect of cold shock on the physiological responses of the cultured mullet, Mugil haematocheilus in winter. Korean J Fish Aquat Sci. 2007;40:226-33. https://doi.org/10.5657/KFAS.2007.40.4.226
  18. Kajita Y, Sakai M, Kobayashi M, Kawaushi H. Enhancement of non-specific cytotoxic activity of leucocytes in rainbow trout Oncorhynchus mykiss injected with growth homone. Fish Shellfish Immunol. 1992;2:155-7. https://doi.org/10.1016/S1050-4648(05)80044-0
  19. Kang SJ, Kwon HC, Kim DJ, Kim MH, Kim Y, Son YC, et al. Fish physiology. Paju: Bioscience; 2007.
  20. Kim JU, Jung SJ. cDNA microarray analysis of viral hemorrhagic septicemia infected olive flounder, Paralichthys olivacus: immune gene expression at different water temperature. J Fish Pathol. 2014;27:1-9. https://doi.org/10.7847/jfp.2014.27.1.001
  21. Kim SM, Kim YJ, Lee DW, Jun LJ, Jeong JB, Park HK, et al. Effects of butaphosphan and cyanocobalamin mixture on immunity and stress in olive flounder, Paralichthys olivaceus. Fish Aquat Sci. 2020;23:26. https://doi.org/10.1186/s41240-020-00171-w
  22. Kumari J, Sahoo PK. Effects of cyclophosphamide on the immune system and disease resistance of Asian catfish, Clarias batrachus. Fish Shellfish Immunol. 2005;19:307-16. https://doi.org/10.1016/j.fsi.2005.01.008
  23. Lopes PA, Pinheiro T, Santos MC, Luz DA, Mathias MDL, Collares-Pereira MJ, et al. Response of antioxidant enzymes in freshwater fish populations (Leuciscus alburnoides complex) to inorganic pollutants exposure. Sci Total Environ. 2001;280:153-63. https://doi.org/10.1016/S0048-9697(01)00822-1
  24. Mechesso AF, Kim YG, Park SC. Effects of butaphosphan and cyanocobalamin combination on plasma immune and biochemical parameters of olive flounder (Paralichthys olivaceus) subjected to crowding stress. Aquacult Res. 2019;50:1611-7. https://doi.org/10.1111/are.14038
  25. NIFS [National Institute of Fisheries Sciences]. Aquatic medicine catalogue [Internet]. 2018 [cited 2019 Jan 4]. http://www.nifs.go.kr/adms/search/goodsList.ad
  26. Palic D, Andreasen CB, Menzel BW, Roth JA. A rapid direct assay to measure degranulation of primary granules in neutrophils from kidney of fathead minnow (Pimephales promelas Rafinesque, 1820). Fish Shellfish Immunol. 2005;19:217-27. https://doi.org/10.1016/j.fsi.2004.12.003
  27. Park JY, Choi JE, Cha SI, Bae NC, Chae PH, Lee JY, et al. Prevalence of alpha1-antitrypsin genotypes in Koreans. Tuberc Respir Dis. 2016;50:229-35. https://doi.org/10.4046/trd.2001.50.2.229
  28. Pereira RA, Siveira PAS, Montagner P, Schneider A, Schmitt E, Rabassa VR, et al. Effect of butaphosphan and cyanocobalmin on postpartum metabolism and milk production in dairy cows. J Anim Biosci. 2003a;7:1143-7.
  29. Pereira RA, Fensterseifer S, Barcelos VB, Martins CF, Schneider A, Schmitt E, et al. Metabolic parameters and dry matter intake of ewes treated with butaphosphan and cyanocobalamin in the early postpartum period. Small Rumin Res. 2013b;114:140-5. https://doi.org/10.1016/j.smallrumres.2013.05.016
  30. Picchietti S, Mazzini M, Taddei AR, Renna R, Fausto AM, Mulero V, et al. Effects of administration of probiotic strains on GALT of larval gilthead seabream: immunohistochemical and ultrastructural studies. Fish Shellfish Immunol. 2007;22:57-67. https://doi.org/10.1016/j.fsi.2006.03.009
  31. Rollin E, Berghaus RD, Rapnicki P, Godden SM, Overton MW. The effect of injectable butaphosphan and cyanocobalamin on postpartum serum β-hydroxybutyrate, calcium, and phosphorus concentrations in dairy cattle. J Dairy Sci. 2010;93:978-87. https://doi.org/10.3168/jds.2009-2508
  32. Seo JS, Lee JH, Park JJ, Choi JS, Bae JS, Lee CW, et al. Biochemical and stress-attenuating effects of butaphosphan-cyanocobalamin combination drug in olive flounder Paralichthys olivaceus. Fish Sci. 2020;86:375-84. https://doi.org/10.1007/s12562-019-01389-x
  33. Siwick i AK, Anderson DP. Nonspecific defense mechanisms assay in fish: II. potential killing activity of neutrophils and macrophages, lysozyme activity in serum and organs and total immunoglobulin (Ig) level in serum. In: Disease diagnosis and prevention methods. Olsztyn, Poland; 1993. Project No.: FAO-project GCP/INT/JPA.
  34. Siwicki AK, Anderson DP, Rumsey GL. Dietary intake of immuostimulants by rainbow trout affects non-specific immunity and protection against furunculosis. Vet Immunol Immunopathol. 1994;41:125-39. https://doi.org/10.1016/0165-2427(94)90062-0
  35. Van der Staay FJ, Groot JDE, Van Reenen CG, Hoving-Bolink AH, Schuurman T, Schmidt BH. Effects of butafosphan on salivary cortisol and behavioral response to social stress in piglets. Vet Pharmacol Therapeut. 2007;30:410-6. https://doi.org/10.1111/j.1365-2885.2007.00884.x
  36. Weber RA, Peleteiro JB, Garcia Martin LO, Aldegunde M. The efficacy of 2-phenoxyethanol, metomidate, clove oil and MS-222 as anesthetic agents in the Senegalese sole (Solea senegalensis Kaup 1858). Aquaculture. 2009;288:147-50. https://doi.org/10.1016/j.aquaculture.2008.11.024
  37. Yeh SP, Chang CA, Chang CY, Liu CH, Cheng W. Dietary sodium alginate administration affects fingerling growth and resistance to Streptococcus sp. and iridovirus, and juvenile non-specific immune responses of the orange-spotted grouper. Fish Shellfish Immunol. 2008;25:19-27. https://doi.org/10.1016/j.fsi.2007.11.011