Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Metabolic profiling reveals an increase in stress-related metabolites in Arabidopsis thaliana exposed to honeybees

  • Baek, Seung-A (Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University) ;
  • Kim, Kil Won (Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University) ;
  • Kim, Ja Ock (Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University) ;
  • Kim, Tae Jin (Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University) ;
  • Ahn, Soon Kil (Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University) ;
  • Choi, Jaehyuk (Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University) ;
  • Kim, Jinho (Division of Chemistry, College of Natural Science, Incheon National University) ;
  • Ahn, Jaegyoon (Department of Computer Science and Engineering, College of Information Technology, Incheon National University) ;
  • Kim, Jae Kwang (Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University)
  • Received : 2021.04.04
  • Accepted : 2021.05.10
  • Published : 2021.06.30
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Abstract

Insects affect crop harvest yield and quality, making plant response mechanisms to insect herbivores a heavily studied topic. However, analysis of plant responses to honeybees is rare. In this study, comprehensive metabolic profiling of Arabidopsis thaliana exposed to honeybees was performed to investigate which metabolites were changed by the insect. A total of 85 metabolites-including chlorophylls, carotenoids, glucosinolates, policosanols, tocopherols, phytosterols, β-amyrin, amino acids, organic acids, sugars, and starch-were identified using high performance liquid chromatography, gas chromatography-mass spectrometry, and gas chromatography-time-of-flight mass spectrometry. The metabolite profiling analysis of Arabidopsis exposed to honeybees showed higher levels of stress-related metabolites. The levels of glucosinolates (glucoraphanin, 4-methoxyglucobrassicin), policosanols (eicosanol, docosanol, tricosanol, tetracosanol), tocopherols (β-tocopherol, γ-tocopherol), putrescine, lysine, and sugars (arabinose, fructose, glucose, mannitol, mannose, raffinose) in Arabidopsis exposed to honeybees were higher than those in unexposed Arabidopsis. Glucosinolates act as defensive compounds against herbivores; policosanols are components of plant waxes; tocopherols act as an antioxidant; and putrescine, lysine, and sugars contribute to stress regulation. Our results suggest that Arabidopsis perceives honeybees as a stress and changes its metabolites to overcome the stress. This is the first step to determining how Arabidopsis reacts to exposure to honeybees.

Keywords

Acknowledgement

This work was supported by Incheon National University Research Grant in 2016.

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