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흑효모를 이용한 참도박 발효 추출물의 항염 효과

Anti-inflammatory effects of Grateloupia elliptica Fermenting Extracts Using Aureobasidium pullulans

  • 부반빈 (영남대학교 생명응용과학대학 생명공학과) ;
  • 이경은 (영남대학교 생명응용과학대학 생명공학과) ;
  • 강상구 (영남대학교 생명응용과학대학 생명공학과)
  • Vu, Van Vinh (Department of Biotechnology, College of Life and applied Sciences, Yeungnam University) ;
  • Lee, Kyung Eun (Department of Biotechnology, College of Life and applied Sciences, Yeungnam University) ;
  • Kang, Sang Gu (Department of Biotechnology, College of Life and applied Sciences, Yeungnam University)
  • 투고 : 2021.02.26
  • 심사 : 2021.04.27
  • 발행 : 2021.06.30

초록

해양 홍조류 참도박(Grateloupia elliptica, G. elliptica)을 흑효모(Aureobasidium pullulans, A. pullulans)로 발효시킨 추출물들의 인간 피부각질세포주(HaCaT)에 대한 항염증 효과를 조사하였다. 흑효모로 발효한 참도박 추출물은 참도박 추출물에 비하여 총 폴리페놀(total polyphenol content)은 최대 2.7 배로 증가되었으며 총 플라보노이드(total flavonoid conten)는 최대 2.4 배 증가되었다. 인간 피부각질세포를 lipopolysaccharide (LPS) 또는 H2O2로 염증을 유도 한 후 참도박 추출물과 참도박 발효 추출물을 100 ㎍/mL 농도로 처리 한 결과 LPS 또는 H2O2 만 처리한 세포군에 비하여 약 3 ~ 10% 정도 세포생존율이 증가되었다. 또한 염증 유발과 관련된 단백질 발현 변화를 조사한 결과 참도박 발효추출물이cyclooxygenase-2 및 70 kDa heat shock protein 의 발현을 현저히 감소시켰다. 따라서 흑효모로 발효한 참도박은 항염효과를 가진 피부수렴용 화장품 소재로 유용하다.

In this study, we investigated the biological functions of Grateloupia elliptica (G. elliptica) fermented with Aureobasidium pullulans (A. pullulans). Total phenolic contents (TPC) of the hot-water extract of the fermented G. elliptica increased 2.7 folds than that of the non-fermented G. elliptica. Furthermore, total flavonoid contents of both the hot-water extract and the ethanol extract increased maximum 2.4 folds amounts than non-fermented G. elliptica extracts.HaCaT cells were induced inflammation treated with LPS (1 ㎍/mL) or H2O2 (1mM) and examined with 100 ㎍/mL of G. elliptica extracts. The extraction of the fermented G. elliptica increased HaCaT cell proliferation in the maximum 10% than non-fermented G. elliptica extraction. Furthermore, investigating changes in protein expression associated with inflammation resulted in a significant reduction in the expression of cyclooxygenase-2 and 70 kDa heat shock proetin. Conclusively, the extracts of G. elliptica fermented with A. pullulans have bioactive functions both anti-oxidant to protect environmental stresses and anti-inflammation activity. Hence, G. elliptica fermented with A. pullulans would be a good natural resource as bioactive ingredients for cosmetics. Therefore, G. elliptica fermented with A. pullulans is useful as a astringent material with anti-inflammatory skin.

키워드

참고문헌

  1. R. Lee, H. Kim, J. M. Yu, Y. H. Cho, D. I. Kim, Y. Shin, Y. Cho, O. J. Kwon, and B. An, Anti-inflammatory effect of Polygonum multiflorum extraction in activated RAW 264.7 cells with lipopolysaccharide, Korean J. Food Preserv., 21(5), 740 (2014). https://doi.org/10.11002/KJFP.2014.21.5.740
  2. K. E. Lee, J. J. Nam, S. M. Kim, H. K. Kim, S. J. Moon, and J. K. Youm, Anti-Inflammatory effects of the mixture of Sorbus commixta, Urtica dioica, Phyllostachys nigra, and Rhus semialata gall extracts on LPS-induced inflammation in HaCaT cells, J. Soc. Cosmet. Sci. Korea, 40(1), 45 (2014). https://doi.org/10.15230/SCSK.2014.40.1.45
  3. L. Ferrero-Miliani, O. H. Nielsen, P. S. Andersen, and S. E. Girardin, Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1beta generation, Clin Exp Immunol, 147(2), 227 (2007). https://doi.org/10.1111/j.1365-2249.2006.03261.x
  4. N. Yahfoufi, N. Alsadi, M. Jambi, and C. Matar, The immunomodulatory and anti-inflammatory role of polyphenols, Nutrients, 10(11), 1618 (2018). https://doi.org/10.3390/nu10111618
  5. J. N. Barker, R. S . Mitra, C. E. Griffiths, V. M. Dixit, and B. J. Nickoloff, Keratinocytes as initiators of inflammation, Lancet., 337(8735), 211 (1991). https://doi.org/10.1016/0140-6736(91)92168-2
  6. B. Zhao, R. Li, F. Yang, F. Yu, N. Xu, F. Zhang, X. Ge and J. Du, LPS -induced vitamin D receptor decrease in oral keratinocytes is associated with oral lichen planus, Sci Rep., 8(1), 763 (2018). https://doi.org/10.1038/s41598-018-19234-z
  7. Y. He, B. G. Kim, H. E. Kim, Q. Chu, S. Shi, G. Ma, Y. Kim, O. S . Kim, and O. J. Kim, The protective role of feruloylserotonin in LPS-induced HaCaT cells, Molecules, 24(17), 3064 (2019). https://doi.org/10.3390/molecules24173064
  8. B. Bertani and N. Ruiz, Function and biogenesis of lipopolysaccharides, EcoSal Plus, 8(1), 10 (2018).
  9. J. Radons, The human HSP70 family of chaperones: where do we stand?, Cell Stress Chaperones, 21(3), 379 (2016). https://doi.org/10.1007/s12192-016-0676-6
  10. T. J. Borges, L. Wieten, M. J. C. V. Herwijnen, F. Broere, R. V. D. Zee, C. Bonorino, and W. V. Eden, The anti-inflammatory mechanisms of Hsp70, Front Immunol, 3, 95 (2012). https://doi.org/10.3389/fimmu.2012.00095
  11. N. Y. Bae, M. J. Kim, K. B. W. R. Kim, N. K. Ahn, Y. U. Choi, J. H. Park, S. H. Park, and D. H. Ahn, Anti-inflammatory effect of ethanol extract from Grateloupia elliptica holmes on lipopolysaccharide-induced inflammatory responses in RAW 264.7 cells and mice ears, J. Korean Soc. Food Sci. Nutr., 44(8), 1128 (2015). https://doi.org/10.3746/JKFN.2015.44.8.1128
  12. K. Y. Kim, K. A. Nam, H. Kurihara, and S. M. Kim, Potent α-glucosidase inhibitors purified from the red alga Grateloupia elliptica, Phytochemistry, 69(16), 2820 (2008). https://doi.org/10.1016/j.phytochem.2008.09.007
  13. J. Kang, S. C. Kim, S. C. Han, H. J. Hong, Y. J. Jeon, B. Kim, Y. S. Koh, E. S. Yoo, and H. K. Kang, Hair-loss preventing effect of Grateloupia elliptica, Biomol. Ther (Seoul), 20(1), 118 (2012). https://doi.org/10.4062/biomolther.2012.20.1.118
  14. M. L. Cho, G. M. Park, S. N. Kim, T. Amna, S. Lee, and W. S. Shin, Glioblastoma-specific anticancer activity of pheophorbide a from the edible red seaweed Grateloupia elliptica, J. Microbiol. Biotechnol., 24(3), 346 (2014). https://doi.org/10.4014/jmb.1308.08090
  15. Z. Chi, F. Wang, Z. Chi, L. Yue, G. Liu, and T. Zang, Bioproducts from Aureobasidium pullulans, a biotechnologically important yeast, Appl. Microbiol. Biotechnol., 82(5), 793 (2009). https://doi.org/10.1007/s00253-009-1882-2
  16. H. Y. Choi, J. D. Kim, and M. Y. Park, A 4 week Randomized, Double-blind human trial to compare the efficacy and safety of Aureobasidium pullulans cultured solution and placebo on improvement of immune in subjects, Korean Journal of Oriental Medicine., 15(3), 83 (2009).
  17. E. K. Park, S . M. Kang, and M. H. Leem, A study on the variation of skin moisture, oil(sebum), melanin and erythema index after application of β-glucan, Kor J Aesthet Cosmetol., 1(3), 83 (2003)
  18. Y. E. Kim, M. A. Yeo, J. H. Han, J. M. Lee, S. K. Jung, H. A. Jeong, S. H. Kim, and J. E. Lee, Aureobasidium pullulans ferment of black tea for anti-oxidation, whitening and anti-wrinkle effects, J. Soc. Cosmet. Sci. Korea, 43(3), 265 (2017). https://doi.org/10.15230/SCSK.2017.43.3.265
  19. Korea Patent 10-0138716 (2016).
  20. C. Cheng, Y. Zhou, M. Lin, P. Wei, and S. T. Yang, Polymalic acid fermentation by Aureobasidium pullulans for malic acid production from soybean hull and soy molasses: Fermentation kinetics and economic analysis, Bioresour. Technol., 223, 166 (2017). https://doi.org/10.1016/j.biortech.2016.10.042
  21. C. An, S . J. Ma, F. Chang, and W. J. Xue, Efficient production of pullulan by Aureobasidium pullulans grown on mixtures of potato starch hydrolysate and sucrose, Braz. J. Microbiol., 48(1), 180 (2017). https://doi.org/10.1016/j.bjm.2016.11.001
  22. S. Suraiya, Y. B. Choi, H. D. Park, W. J. Jang, H. H. Lee, and I. S. Kong, Saccharina japonica fermented by Monascus spp. inhibit adipogenic differentiation and gene expression analyzed by real-time PCR (Q-PCR) in 3T3-L1 cell, J. Funct. Foods, 55, 371 (2019). https://doi.org/10.1016/j.jff.2019.02.043
  23. Korea Patent 10-1647049 (2016).
  24. Korea Patent 10-0893350 (2009).
  25. A. Sushant, B. K. Manoj, D. Krisha, K. Puspa, G. Roshani, and K. Niranjan, Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from western nepal, Plants (Basel), 8(4), 96 (2019). https://doi.org/10.3390/plants8040096
  26. V. V. Vu, K. E. Lee, and S . G. Kang, Evaluation of antioxidant, tyrosinase and collagenase inhibitory of Grateloupia elliptica extracts after Aureobasidium pullulans fermentation, J. Soc. Cosmet. Sci. Korea, 46(1), 1 (2020). https://doi.org/10.15230/SCSK.2020.46.1.1