Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Biphasic immunomodulatory effects of ionized biosilica water on the antigen-presenting capability of mouse dendritic cells

마우스 수지상세포의 항원 제시 능력에 대한 이온화 규소수의 biphasic 면역조절 효과

  • Lee, You-Jeong (Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University) ;
  • Joo, Hong-Gu (Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University)
  • 이유정 (제주대학교 수의과대학 수의약리학실) ;
  • 주홍구 (제주대학교 수의과대학 수의약리학실)
  • Received : 2021.02.08
  • Accepted : 2021.03.22
  • Published : 2021.06.30
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Abstract

Biosilica is a silica-based substance derived from the cell walls (frustules) of diatoms. Recently, research into biosilica's biological functions is underway, but little has been reported on the effects of biosilica on immune cells. In this study, we investigated the effect of ionized biosilica water (iBW) on dendritic cells (DCs), which play crucial roles as antigen (Ag)-presenting cells. Treatment with iBW increased the expression of immune response-related markers, closely connected to the maturation of DCs, and the production of tumor necrosis factor-alpha. In addition, iBW-treated DCs (iBW-DCs) had a lower uptake of fluorescein isothiocyanate-dextran than that of control DCs. Mixed leukocyte response analysis used for measuring the Ag-presenting capability of DCs, showed iBW-DCs had a higher capability than that of control DCs. Interestingly, DCs treated with lipopolysaccharide (LPS) and iBW had a lower level of Ag-presenting capability than that of LPS-treated DCs. Taken together, the results indicate that iBW alone can mature DCs, but it decreases the Ag-presenting capability of DCs in the presence of LPS, a representative agent of inflammation. This study may provide valuable information regarding the effect of iBW on immune cells. Further research is needed to investigate how iBW induces the observed biphasic immunomodulatory activity.

Keywords

Acknowledgement

이 논문은 2020년도 정부(산업통상자원부)의 재원으로 한국산업기술진흥원의 지원을 받아 수행된 연구임(N0002327, 2020년 산학융합지구 조성사업).

References

  1. Maher S, Kumeria T, Aw MS, Losic D. Diatom silica for biomedical applications: recent progress and advances. Adv Healthc Mater 2018;7:e1800552.
  2. Lee J, Lee HA, Shin M, Juang LJ, Kastrup CJ, Go GM, Lee H. Diatom frustule silica exhibits superhydrophilicity and superhemophilicity. ACS Nano 2020;14:4755-4766. https://doi.org/10.1021/acsnano.0c00621
  3. Uthappa UT, Brahmkhatri V, Sriram G, Jung HY, Yu J, Kurkuri N, Aminabhavi TM, Altalhi T, Neelgund GM, Kurkuri MD. Nature engineered diatom biosilica as drug delivery systems. J Control Release 2018;281:70-83. https://doi.org/10.1016/j.jconrel.2018.05.013
  4. Cicco SR, Vona D, Leone G, De Giglio E, Bonifacio MA, Cometa S, Fiore S, Palumbo F, Ragni R, Farinola GM. In vivo functionalization of diatom biosilica with sodium alendronate as osteoactive material. Mater Sci Eng C Mater Biol Appl 2019;104:109897. https://doi.org/10.1016/j.msec.2019.109897
  5. Pernis B. Silica and the immune system. Acta Biomed 2005;76 Suppl 2:38-44.
  6. Chen L, Liu J, Zhang Y, Zhang G, Kang Y, Chen A, Feng X, Shao L. The toxicity of silica nanoparticles to the immune system. Nanomedicine (Lond) 2018;13:1939-1962. https://doi.org/10.2217/nnm-2018-0076
  7. den Haan JM, Arens R, van Zelm MC. The activation of the adaptive immune system: cross-talk between antigen-presenting cells, T cells and B cells. Immunol Let 2014;162:103-112. https://doi.org/10.1016/j.imlet.2014.10.011
  8. Banchereau J, Steinman RM. Dendritic cells and the control of immunity. Nature 1998;392:245-252. https://doi.org/10.1038/32588
  9. Mellman I. Dendritic cells: master regulators of the immune response. Cancer Immunol Res 2013;1:145-149. https://doi.org/10.1158/2326-6066.CIR-13-0102
  10. Guermonprez P, Valladeau J, Zitvogel L, Thery C, Amigorena S. Antigen presentation and T cell stimulation by dendritic cells. Annu Rev Immunol 2002;20:621-667. https://doi.org/10.1146/annurev.immunol.20.100301.064828
  11. Han Y, Joo HG. Determination of the toxicity of enrofloxacin on mouse bone marrow cells. J Prev Vet Med 2019;43:141-145. https://doi.org/10.13041/jpvm.2019.43.4.141
  12. Jang YR, Yoon HR, Kim MH, Joo HG. Anti-inflammatory activity of doxorubicin on lipopolysaccharide-induced hyperactivation of spleen cells. J Prev Vet Med 2018;42:61-65. https://doi.org/10.13041/jpvm.2018.42.2.61
  13. Lee YJ, Go GM, Joo HG. Immunomodulatory activity of biosilica water on mouse dendritic cells. J Prev Vet Med 2020;44:156-160. https://doi.org/10.13041/jpvm.2020.44.4.156
  14. Blanco P, Palucka AK, Pascual V, Banchereau J. Dendritic cells and cytokines in human inflammatory and autoimmune diseases. Cytokine Growth Factor Rev 2008;19:41-52. https://doi.org/10.1016/j.cytogfr.2007.10.004
  15. Plat CD, Ma JK, Chalouni C, Ebersold M, Bou-Reslan H, Carano R, Mellman I, Delamarre L. Mature dendritic cells use endocytic receptors to capture and present antigens. Proc Natl Acad Sci U S A 2010;107:4287-4292. https://doi.org/10.1073/pnas.0910609107
  16. Tourkova IL, Yurkovetsky ZR, Shurin MR, Shurin GV. Mechanisms of dendritic cell-induced T cell proliferation in the primary MLR assay. Immunol Let 2001;78:75-82. https://doi.org/10.1016/S0165-2478(01)00235-8